Determination method for β-lactam antibiotic residues in milk

A technology of lactams and detection methods, which is applied in the direction of analyzing materials through chemical reactions and observing the impact on chemical indicators, etc. It can solve the problems of cumbersome operation steps, long detection time, poor selectivity, etc. problem, to achieve the effect of simple operation method, fast detection speed and high accuracy

Inactive Publication Date: 2011-12-07
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned methods have their own strengths in detection precision, etc., some have low sensitivity and poor selectivity; some operation steps are more cumbersome, and some need to purchase expensive instruments, but most of them have the disadvantages of long detection time and high cost. Processing enterprises cause a lot of inconvenience

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. 100 milliliters of antibiotic-free milk (blank) samples and 100 milliliters of milk samples to be tested are mixed with 50 milliliters of acidified acetonitrile respectively, and then centrifuged at 3000 r / min for 3 min. Said acidified acetonitrile is composed of hydrochloric acid: acetonitrile by volume = 1:1 prepared;

[0022] 2. Then use 1% dilute sulfuric acid to adjust the pH value of the centrifuged sample supernatant to 6, add 0.1g / L copper sulfate solution according to the volume ratio of the supernatant to 1:2, shake well and let it stand for 15 minutes to react;

[0023] 3. Use a UV spectrophotometer to test the UV absorbance value of the supernatant after the reaction at 240nm, record and compare the data to determine whether there are β-lactam antibiotics left in the milk sample to be tested. Record the data and calculate that the content of β-lactam antibiotics in the blank sample is 0.01mg / L, the content of β-lactam antibiotics in the sample to be teste...

Embodiment 2

[0025] 1. 100 milliliters of antibiotic-free milk (blank) samples and 100 milliliters of milk samples to be tested were mixed with 200 milliliters of acidified acetonitrile respectively, and then centrifuged at 800 r / min for 10 min. Said acidified acetonitrile was composed of hydrochloric acid: acetonitrile by volume ratio = 1:2 prepared;

[0026] 2. Then use 10% dilute sulfuric acid to adjust the pH value of the centrifuged sample supernatant to 2, add 2g / L lead acetate solution according to the volume ratio of the supernatant to 1:2, shake well, and let it stand for 5 minutes;

[0027] 3. Use a UV spectrophotometer to test the UV absorbance value of the supernatant after the reaction at 380nm, record and compare the data to determine whether there are β-lactam antibiotics left in the milk sample to be tested.

[0028] Record the data and calculate that the blank sample β-lactam antibiotic content is 0.85mg / L, the test sample β-lactam antibiotic content is 1.04mg / L, and the y...

Embodiment 3

[0030] 1, 100 milliliters of antibiotic-free milk (blank) samples and 100 milliliters of milk samples to be tested were mixed with 100 milliliters of acidified acetonitrile respectively, and then centrifuged at 1500 r / min for 8 min. Said acidified acetonitrile was composed of hydrochloric acid: acetonitrile by volume ratio = 1:5 prepared;

[0031] 2. Use 6% dilute sulfuric acid to adjust the pH value of the supernatant of the centrifuged sample to 3, add 1g / L cobalt chloride solution according to the volume ratio of the supernatant to 1:2, shake well, and let it stand for 8 minutes;

[0032] 3. Use a UV spectrophotometer to test the UV absorbance value of the supernatant after the reaction at 320nm, record and compare the data to determine whether there are β-lactam antibiotics left in the milk sample to be tested.

[0033] Record the data and calculate that the blank sample β-lactam antibiotic content is 0.93mg / L, the test sample β-lactam antibiotic content is 1.21mg / L, and t...

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Abstract

The invention provides a method for detecting β-lactam antibiotic residues in milk. The residues of β-lactam antibiotics in milk are detected by three steps of centrifugation, chemical reaction of β-lactam antibiotics and heavy metals, and UV spectrophotometry. The damage is small; the detection speed is fast, and the entire operation process can be completed within 30 minutes; no need to purchase expensive antibiotic detection instruments, only a low-speed centrifuge and a UV spectrophotometer can be used to complete the detection.

Description

technical field [0001] The invention relates to a method for detecting residues of beta-lactam antibiotics in milk, in particular to a qualitative and preliminary quantitative method for rapid detection of residues of beta-lactam antibiotics in milk. Background technique [0002] Milk is a liquid food with the most complete nutrients and the highest nutritional value after human breast milk. Milk is rich in protein, fat, amino acid, sugar, salt, calcium, phosphorus, iron and other nutrients, as well as vitamins, enzymes and antibodies, etc. It is most easily digested and absorbed by the human body. However, there are antibiotic problems in milk in actual production. Antibiotics with β-lactam rings such as penicillin G, amoxicillin, ampicillin, cephalosporins, and cloxacillin can be used as efficient veterinary drugs in the dairy industry. To treat diseases such as mastitis, with the improvement of living standards, the problem of antibiotic residues in milk has been paid mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 杨亚玲张丹扬张尹刘谋盛
Owner KUNMING UNIV OF SCI & TECH
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