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Sub-totipotent stem cell, preparation method and application thereof

A technology of subpluripotent stem cells and uses, applied in the field of subpluripotent stem cells, the field of preparation of the subpluripotent stem cells, can solve the problems of little understanding of stem cells and so on

Active Publication Date: 2014-07-09
BEIJING HEALTH & BIOTECH (H&B) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, people's understanding and clinical use of multipotent stem cells such as hematopoietic stem cells is relatively deep, and the understanding of embryonic stem cells has also made great progress. Many characteristics of stem cells, but also have many biological characteristics of pluripotent stem cells, transplanted into the body will not produce teratomas, such a type of stem cells are poorly understood

Method used

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  • Sub-totipotent stem cell, preparation method and application thereof
  • Sub-totipotent stem cell, preparation method and application thereof
  • Sub-totipotent stem cell, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Isolation of CD151 by adherent subculture + CD184 - Oct4 + subtotipotent stem cells

[0074] Take cut human placenta, carry out CD151 + CD184 - Oct4 + Preparation of stem cell products. The specific steps are as follows: 1) Aseptically collect the cut human placenta; 2) Cut to small fragments 3 Small tissue pieces were digested with 0.5 mg / mL collagenase Ⅰ at 37°C for 1 hour, and 1 mg / mL trypsin at 37°C for 30 minutes, during which they were gently shaken 5 times, sieved, centrifuged to collect the cell suspension, and inoculated in T75cm 2 In the culture flask, cultured in cell culture solution A for 5 to 7 days, the spindle-like cells adhered to the wall; 3) The adherent growth cells were digested with trypsin and further cultured, passaged, purified, expanded and passaged for 6 generations, and the cells were harvested. After concentrating and preparing a cell suspension with cell preservation solution, some cells are sent for quality inspection, and the rest a...

Embodiment 2

[0078] Isolation of CD151 by adherent subculture + CD184 - Oct4 + subtotipotent stem cells

[0079] Same as Example 1, except that the cell culture medium used is cell culture medium B.

[0080] The results obtained were substantially the same as in Example 1.

Embodiment 3

[0082] Isolation of CD151 by adherent subculture + CD184 - Oct4 + subtotipotent stem cells

[0083] Same as Example 1, except that the cell culture medium used is cell culture medium C.

[0084] The results obtained were substantially the same as in Example 1.

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Abstract

The invention discloses a sub-totipotent stem cell from cut human umbilical cord or placenta, wherein the cell marker is CD151<+>OCT4<+>CD184<->; the cell grows adhering to the wall in a culture container and can differentiate to the body entoderm, mesoblast and ectoderm tissues. The invention also discloses a preparation method of the sub-totipotent stem cell and an application thereof for preparing drug for treating cell injuries or cell aging diseases, and an application thereof as a carrier cell of a gene treatment drug.

Description

technical field [0001] The present invention relates to the field of stem cells, in particular to a subtotipotent stem cell derived from cut human umbilical cord or placenta. At the same time, the present invention also relates to the preparation method of the sub-totipotent stem cells and the use of the sub-totipotent stem cells. Background technique [0002] The human body is made up of trillions of cells. Cells are the basic structural and functional units of the human body, which are differentiated from stem cells, so stem cells are the origin cells of various tissues and organs in the human body. Stem cells are characterized by self-replication and multilineage differentiation. During normal reproductive physiology, stem cells are differentiated from fertilized eggs. The fertilized egg initially develops into primitive embryonic stem cells, which have the full potential to form a complete individual. Primitive embryonic stem cells further differentiate and prolifera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0735A61K48/00A61P39/06A61P7/06A61P39/00A61P37/02A61P25/00A61P37/06
Inventor 赵宝娜
Owner BEIJING HEALTH & BIOTECH (H&B) CO LTD
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