Preparation method and application of magnetic sandwich nano immunosensor
An immune sensor and nanotechnology, applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problems of uncontrollable antibody orientation, low sensor sensitivity, antibody loss of activity, etc., achieve good immobilization effect, improve sensitivity, and stabilize detection performance Effect
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[0030] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
1. Specific examples
[0031] A method for preparing a magnetic sandwich nano-immunosensor of the present invention specifically comprises the following steps:
[0032] (1) Preparation of secondary antibody probe
[0033] a.Fe 3 o 4 / Preparation of Au gel nano magnetic beads
[0034] a1. 100mL of 0.01wt% chloroauric acid (HAuCl 4 ) placed in a beaker to heat, after boiling, quickly add 2.5mL of 1wt% trisodium citrate, continue to boil for 10min until the solution turns dark red, stir slightly and cool to obtain a colloidal gold solution;
[0035] a2. Add 0.2g of ferric oxide into 90mL of 50wt% ethanol solution and ultrasonically disperse evenly, then add 300uL of 3-mercaptopropyltriethoxysilane (3-MPTES) and 500uL of ammonia water, in a constant temperature water bath at 60°C, Pass N 2 After protection and reaction for 6 hours, wash wit...
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