Method for measuring electrophysiological activity of colon muscles of mice

A measurement method and technology of physiological activity, applied in the directions of diagnostic recording/measurement, medical science, sensors, etc., can solve the problems of difficult realization of mice and animals, and achieve the effect of high success rate, slight loss and good repeatability

Inactive Publication Date: 2012-10-17
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the electrode implantation technology in rats has been reported in public literature, but this technology is difficult to achieve in mice

Method used

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  • Method for measuring electrophysiological activity of colon muscles of mice
  • Method for measuring electrophysiological activity of colon muscles of mice
  • Method for measuring electrophysiological activity of colon muscles of mice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Establishment of constipation mouse model

[0036] 1.1 Grouping 20 mice, both male and female, were randomly divided into two groups after weighing, the control group and the constipation group, 10 in each group.

[0037] 1.2 Preparation of medicaments Take 2 tablets of compound diphenoxylate tablets and add 5ml of distilled water to make a suspension. Take 2g of eosin and add 10ml of distilled water to make a suspension.

[0038] 1.3 Modeling: Food and water were stopped 24 hours before the test, and the constipation group was given 10 mg / kg compound diphenoxylate suspension orally to replicate the constipation model. The control group was replaced by a suspension of water and starch. The number of feces particles, the shape of feces and the body weight of mice were recorded every day, and the dosage was appropriately increased or decreased according to the changes in feces and body weight. The feeding time was 30 days.

[0039] 2. Experimental indicators...

Embodiment 2

[0043] Example 2 Determination of Colon Myoelectric Activity in Constipated Mice

[0044] 1. Experimental process After 30 days of successful modeling, the colonic myoelectricity of the mice in the constipation group and the control group was measured. The mice were fasted for 24 hours, anesthetized by intraperitoneal injection of 20% urethane (0.3ml / mouse), then lay supine on the operating table, routinely disinfected, cut the abdomen 1.5cm longitudinally along the middle of the lower abdomen, exposed the ileocecal, and placed it in the proximal colon (about 1cm away from the cecum) place a pair of copper wire electrodes (the two electrodes are 0.5cm apart, inserted into the seromuscular layer), and the wires are drawn out through the incision to connect to the BL-410 biological function experiment system. Recording started after 30 min. Sensitivity selection: gain magnification 500, time constant 0.001s, high-frequency filter 30HZ, continuous recording for 45min, repeated r...

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Abstract

The invention discloses a method for measuring electrophysiological activity of colon muscles of mice and application thereof. The method comprises the following steps of: performing gavage on the mice by using compound diphenoxylate suspension to establish a constipation model, selecting medical suture needles, manufacturing copper wires into copper wire electrodes, and measuring the change of electrophysiological activity of colon. Compared with the result of the control group, the result shows that the slow wave colon frequency, the frequency variation coefficient, the amplitude and the amplitude variation coefficient of the mice in a constipation group have an extremely obvious difference P which is less than 0.01. The method for measuring the electrophysiological activity of colon muscles of mice has the advantages that 1, the measuring method is easily available in raw materials, simple, practicable, high in repeatability and high in success rate; 2, the method is slight in damage to a subject and suitable for mice and other animals; 3, according to the measuring method, the change of the electrophysiological activity of colon muscles of the mice can be objectively reflected; and 4, a powerful tool is provided for researching the movement mechanism of the colon and controlling constipation in the system.

Description

technical field [0001] The invention relates to the technical field of medical evaluation and detection, in particular to a method for measuring electrophysiological activity of colon myocardium in rats and its application. Background technique [0002] With the improvement of people's living standards and the quickening of the pace of work, the incidence of constipation is increasing year by year. Constipation is a common clinical symptom, and the exact etiology is unknown at present. According to research, the number of propulsive contraction waves of the colon in patients with constipation is reduced, and the contraction amplitude is reduced, resulting in slower passage of feces in the colon. Because of the important role of the colon in efficiently moving stool out of the body, constipation is often attributed to abnormal colon motility. [0003] The myoelectric action potential of the colon is generated on the slow wave, which is consistent with the contraction of smo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/0488
Inventor 杜爱林姜洪波侯软玲郭永臣卢智敏宋亭亭李多多杨俊刘晓丽路承彪谭军沈慧安娜徐磊宦楠楠连阳阳余意赵东海洪渊
Owner XINXIANG MEDICAL UNIV
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