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Device and method for detecting electron transfer in process of electrically promoted microorganism denitrification

A technology of electron transfer and denitrification, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc. Fine, ingenious device design, the effect of shortening the film hanging time

Inactive Publication Date: 2014-05-07
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These types of reactors are commonly used in wastewater treatment, and can effectively denitrify wastewater, but in the reaction process, the biofilm is too dispersed to effectively detect the transfer of electrons in the reverse process.

Method used

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  • Device and method for detecting electron transfer in process of electrically promoted microorganism denitrification
  • Device and method for detecting electron transfer in process of electrically promoted microorganism denitrification
  • Device and method for detecting electron transfer in process of electrically promoted microorganism denitrification

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Electron transfer process or rate and denitrification and denitrification effect of electrocatalyzed organisms in the device under different voltages.

[0043] Set different voltage values ​​of 1V, 2V, 3V and 3.5V respectively, inject 6mL of nutrient solution into the reaction device (composition of nutrient solution: 30mg / L NO 3 - -N, 80mg / L NaHCO 3 , 5g / LK 2 HPO 4 , 1.5g / L KH 2 PO 4 ), after the current is stable, inject 4ml of energized and domesticated microorganisms with a concentration of 10mg / ml into the device, and the final concentration of microorganisms in the reaction system is 4mg / ml. and the current change value of the recording device, and the reaction is ended after the current basically tends to be stable, and the total reaction time is about 2.5 hours. Finally, the nutrient solution in the device is poured out, and the water quality index in the nutrient solution is measured by a standard method, wherein NO 3 - -N was determined by UV spectroph...

Embodiment 2

[0046] Electron transfer process and rate of electrocatalyzed organisms and denitrification and nitrogen removal in the device under different final concentrations of microorganisms. Set the voltage value to be constant at 3V, inject 6mL of nutrient solution into the reaction device (composition of nutrient solution: 30mg / L NO 3 - -N, 80mg / L NaHCO 3 , 5g / L K 2 HPO 4 , 1.5g / LKH 2 PO 4 ), after the current is stable, inject 1mL, 2mL, 3mL, 4mL and 5mL of energized and domesticated microorganisms with a concentration of 10mg / ml into the device respectively, and the final concentrations of microorganisms after injection are 1.4mg / ml and 2.5mg / ml respectively , 3.3mg / ml, 4mg / ml and 4.5mg / ml. The microorganisms are taken from a stable electrode biofilm reactor. Observe and record the current change value of the device. After the current is basically stable, the reaction is ended. The total reaction time is about 2.5 hours, and the nutrient solution leveling time is 0.5 hours. A...

Embodiment 3

[0050] Electron transfer process and rate of electrocatalyzed organisms and denitrification and nitrogen removal in the device under different types of microorganisms. Set the voltage value to be constant at 3V, inject 6mL of nutrient solution into the reaction device (composition of nutrient solution: 30mg / L NO 3 - -N, 80mg / L NaHCO 3 , 5g / L K 2 HPO 4 , 1.5g / LKH 2 PO 4 ), after the current is stable, inject 5mL of energized and domesticated microorganisms with a concentration of 10mg / ml and non-energized domesticated microorganisms into the device respectively. The final concentration of microorganisms in the reaction system after injection is 4.5mg / ml. The electrode biofilm reactor (acclimated with electricity) and the control reactor with only microorganisms (acclimated with electricity) were taken from the stable operation electrode biofilm reactor (acclimated with electricity), and the current change value in the device was observed and recorded. After the current bas...

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Abstract

The invention discloses a device and a method for detecting electron transfer in a process of electrically promoted microorganism denitrification. The device comprises a base, a cathode electrode plate and a reaction tank stacked from bottom to top, wherein the reaction tank is provided with a liquid injection cavity; an anode electrode plate is inserted in the liquid injection cavity; a through hole is arranged at the bottom of the liquid injection cavity; and the bottom of the through hole is closed by the cathode electrode plate. When the device works, a power source is powered on; the liquid injection cavity is injected with wastewater; when the current value is stable, the microorganism is injected in the liquid injection cavity; the microorganism drops onto the cathode electrode plate to form a biomembrane; and then, the electrically promoted microorganism denitrification reaction is performed; the current change is monitored in real time; when the current value is stable, the reaction is ended; the water body indexes of the wastewater are detected; and the electron transfer process and speed rate in the reaction process are determined according to the current value change and the final water body indexes. The device can be used for effectively researching the electron transfer process and speed rate between the microorganism and the electrodes in the electrically promoted microorganism reinforcing system.

Description

technical field [0001] The invention relates to the technical field of electro-accelerated biological denitrification denitrification treatment, in particular to a device and method for detecting electron transfer in an electro-accelerated biological denitrification denitrification process. Background technique [0002] In recent years, electro-accelerated bioaugmentation technology, as a new water treatment technology, has become a research hotspot at home and abroad in terms of denitrification and denitrification. At present, the research of this technology is mainly focused on the design of the reaction device based on the improvement of removal efficiency and the investigation of different factors affecting the denitrification effect. The research on the electron transfer mechanism between microorganisms and electrodes, how the micro-electric field affects microbial activity and microbial analysis has not. Relatively small. [0003] Existing studies have found that: on ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/12C02F3/34
Inventor 丛燕青徐谦冯华军沈东升
Owner ZHEJIANG GONGSHANG UNIVERSITY
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