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Device and method for detecting electron transfer in process of electrically promoted microorganism denitrification

An electron transfer and denitrification technology, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of biofilm dispersion and ineffective detection of electron transfer, and achieve the goal of making Fine, ingenious design of the device, to solve the effect of shedding

Inactive Publication Date: 2012-10-17
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These types of reactors are commonly used in wastewater treatment, and can effectively denitrify wastewater, but in the reaction process, the biofilm is too dispersed to effectively detect the transfer of electrons in the reverse process.

Method used

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  • Device and method for detecting electron transfer in process of electrically promoted microorganism denitrification
  • Device and method for detecting electron transfer in process of electrically promoted microorganism denitrification
  • Device and method for detecting electron transfer in process of electrically promoted microorganism denitrification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The electron transfer process or rate and the denitrification and denitrification effect of electricity in the device under different voltages.

[0043] Set different voltage values ​​to 1V, 2V, 3V and 3.5V respectively, and inject 6mL of nutrient solution into the reaction device (the composition of the nutrient solution: 30mg / L NO 3 - -N, 80mg / L NaHCO 3 , 5g / LK 2 HPO 4 , 1.5g / L KH 2 PO 4 ), after the current stabilizes, inject 4ml of energized and domesticated microorganisms with a concentration of 10mg / ml into the device. The final concentration of microorganisms in the reaction system is 4mg / ml. The microorganisms are taken from a stable operating electrode biofilm reactor. Observe And the current change value of the recording device, the reaction is ended after the current basically stabilizes, and the total reaction time is about 2.5 hours. Finally, the nutrient solution in the device is poured out, and the water quality index in the nutrient solution is measured usin...

Embodiment 2

[0046] The process and rate of electron transfer and denitrification and denitrification of the electro-promoted organisms in the device under different final concentrations of microorganisms. Set the voltage value to be constant at 3V, and inject 6 mL of nutrient solution into the reaction device (the composition of the nutrient solution: 30mg / L NO 3 - -N, 80mg / L NaHCO 3 , 5g / L K 2 HPO 4 , 1.5g / LKH 2 PO 4 ), after the current stabilizes, inject 1mL, 2mL, 3mL, 4mL and 5mL of the energized and domesticated microorganisms with a concentration of 10mg / ml respectively into the device. The final concentration of the microorganisms after injection is 1.4mg / ml and 2.5mg / ml respectively , 3.3mg / ml, 4mg / ml and 4.5mg / ml. The microorganisms are taken from a stable electrode biofilm reactor, and the current change value of the device is observed and recorded. The reaction is completed after the current basically stabilizes. The total reaction time is about 2.5 hours, of which the nutrient s...

Embodiment 3

[0050] The electron transfer process and rate of the electro-promoted organisms and the denitrification and denitrification situation in the device under different types of microorganisms. Set the voltage value to be constant at 3V, and inject 6 mL of nutrient solution into the reaction device (the composition of the nutrient solution: 30mg / L NO 3 - -N, 80mg / L NaHCO 3 , 5g / L K 2 HPO 4 , 1.5g / LKH 2 PO 4 ), after the current stabilizes, inject 5 mL of energized domesticated microorganisms and non-energized domesticated microorganisms with a concentration of 10 mg / ml into the device. After injection, the final concentration of the microorganisms in the reaction system is 4.5 mg / ml, and the microorganisms are respectively Taken from a stable operating electrode biofilm reactor (electrical acclimation) and a control reactor with only microorganisms (non-electric acclimation). Observe and record the current change value in the device. After the current basically stabilizes, the reactio...

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Abstract

The invention discloses a device and a method for detecting electron transfer in a process of electrically promoted microorganism denitrification. The device comprises a base, a cathode electrode plate and a reaction tank stacked from bottom to top, wherein the reaction tank is provided with a liquid injection cavity; an anode electrode plate is inserted in the liquid injection cavity; a through hole is arranged at the bottom of the liquid injection cavity; and the bottom of the through hole is closed by the cathode electrode plate. When the device works, a power source is powered on; the liquid injection cavity is injected with wastewater; when the current value is stable, the microorganism is injected in the liquid injection cavity; the microorganism drops onto the cathode electrode plate to form a biomembrane; and then, the electrically promoted microorganism denitrification reaction is performed; the current change is monitored in real time; when the current value is stable, the reaction is ended; the water body indexes of the wastewater are detected; and the electron transfer process and speed rate in the reaction process are determined according to the current value change and the final water body indexes. The device can be used for effectively researching the electron transfer process and speed rate between the microorganism and the electrodes in the electrically promoted microorganism reinforcing system.

Description

Technical field [0001] The invention relates to the technical field of electrical-assisted biological denitrification and denitrification treatment, in particular to a device and method for detecting electron transfer in the process of electrically-accelerated biological denitrification and denitrification. Background technique [0002] In recent years, as a new type of water treatment technology, electro-assisted bioaugmentation technology has become a research hotspot at home and abroad in terms of denitrification and nitrogen removal. At present, the research of this technology mainly focuses on the design of the reaction device based on improving the removal efficiency and the investigation of different factors affecting the denitrification effect. The research on the electron transfer mechanism between the microorganism and the electrode, how the micro-electric field affects the activity of the microorganism and the analysis of the microorganism, etc. Relatively small. [000...

Claims

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Application Information

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IPC IPC(8): G01N27/12C02F3/34
Inventor 丛燕青徐谦冯华军沈东升
Owner ZHEJIANG GONGSHANG UNIVERSITY
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