Preparation and application for accelerating Gram-negative current-producing bacteria anode bio-film efficiency in microbial fuel cell

A gram-negative, fuel cell technology, used in biochemical fuel cells, biological water/sewage treatment, electrochemical-biocombination treatment, etc. The effect of low cost of use, shortened film hanging time, and wide practicability

Active Publication Date: 2017-10-24
ZHEJIANG GONGSHANG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In view of the fact that some Gram-negative bacteria have good electricity production performance in the prior art, but they have the problems of low film formation efficiency, high cost and limited applicability on metal electrodes, the present invention provides an accelerated microbial fuel cell Preparation and method for the anode film-hanging efficiency of middle-grade gram-negative electrogenic bacteria

Method used

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  • Preparation and application for accelerating Gram-negative current-producing bacteria anode bio-film efficiency in microbial fuel cell
  • Preparation and application for accelerating Gram-negative current-producing bacteria anode bio-film efficiency in microbial fuel cell
  • Preparation and application for accelerating Gram-negative current-producing bacteria anode bio-film efficiency in microbial fuel cell

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Experimental program
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Effect test

Embodiment 1

[0107] (1) Preparation of preparation: Weigh all the components required by various signal molecules according to the mass percentage of the components (C4-HSL10%, C6-HSL10%, C8-HSL 10%, C10-HSL10%, C12-HSL10% , 3-oxo-6-HSL10%, 3-oxo-C8-HSL10%, 3-oxo-C10-HSL10%, 3-oxo-C12-HSL10%, pC-HSL10%), dissolved in ethyl acetate and mixed well , and then blow off by nitrogen, drop into ammonium chloride and dimethyl sulfoxide weighed in component mass percentage and stir evenly (signal molecule 50%, ammonium chloride 30%, dimethyl sulfoxide 10%, signal molecule, Ammonium chloride and dimethyl sulfoxide are pressed into tablet preparations, tablet specifications: diameter is 2cm, thickness 5mm. Coating liquid is configured, and each component weighs ethyl cellulose 60%, phthalate Diethyl formate 30%, PEG600010%. Utilize rolling spray coating technology, in preparation outsourcing with coating film. Finished product (preparation flow process such as figure 2 shown).

[0108] (2) Microbi...

Embodiment 2

[0117] (1) Preparation of preparation: Weigh all the components required by various signal molecules according to the mass percentage of the components (C4-HSL10%, C6-HSL10%, C8-HSL 10%, C10-HSL10%, C12-HSL10% , 3-oxo-6-HSL10%, 3-oxo-C8-HSL10%, 3-oxo-C10-HSL10%, 3-oxo-C12-HSL10%, pC-HSL10%), dissolved in ethyl acetate and mixed well , then blow off by nitrogen, drop into ammonium chloride and dimethyl sulfoxide weighed in component mass percentage and stir evenly (signal molecule 50%, ammonium chloride 30%, dimethyl sulfoxide 10%, coating solution 10% %), the signal molecule, ammonium chloride and dimethyl sulfoxide were compressed into a tablet preparation, the tablet specification: diameter 2cm, thickness 5mm. A coating liquid is prepared, and each component is weighed by mass percentage of 60% of ethyl cellulose, 30% of diethyl phthalate, and 10% of PEG6000. Utilize the rolling spray coating technology to coat the preparation with a coating film. After passing the inspect...

Embodiment 3

[0127] (1) Preparation of preparation: Weigh all the components required by various signal molecules according to the mass percentage of the components (C4-HSL10%, C6-HSL10%, C8-HSL 10%, C10-HSL10%, C12-HSL10% , 3-oxo-6-HSL10%, 3-oxo-C8-HSL10%, 3-oxo-C10-HSL10%, 3-oxo-C12-HSL10%, pC-HSL10%), dissolved in ethyl acetate and mixed well , then blow off by nitrogen, drop into ammonium chloride and dimethyl sulfoxide weighed in component mass percentage and stir evenly (30% of signal molecule, 50% of ammonium chloride, 10% of dimethyl sulfoxide, coating solution 10% %), the signal molecule, ammonium chloride and dimethyl sulfoxide were compressed into a tablet preparation, the tablet specification: diameter 2cm, thickness 5mm. A coating liquid is prepared, and each component is weighed by mass percentage of 60% of ethyl cellulose, 30% of diethyl phthalate, and 10% of PEG6000. Utilize the rolling spray coating technology to coat the preparation with a coating film. After passing th...

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Abstract

The invention discloses preparation and application for accelerating Gram-negative current-producing bacteria anode bio-film efficiency in a microbial fuel cell. The anode bio-film is prepared from the following components in percentage by mass: 10-60% of a signal molecule, 10-60% of an ammonium salt, 10-20% of a signal molecule protective agent and 10-20% of a sustained-release agent. The preparation method comprises the following steps: feeding to-be-treated wastewater into an anode chamber of the microbial fuel cell, sequentially adding the preparation capable of accelerating the Gram-negative current-producing bacteria anode bio-film efficiency and inoculating Gram-negative current-producing bacteria synchronously or according to any sequence. The preparation disclosed by the invention is capable of improving the bio-film growth speed and shortening the forming time of the bio-film, has the characteristics of low cost and wide applicability and lays a foundation for expanded engineering application of the microbial fuel cell.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, and more specifically relates to a preparation for accelerating the membrane-hanging efficiency of Gram-negative electrogenic bacteria in microbial fuel cells. Background technique [0002] Energy shortage and environmental pollution are two major problems facing China today. Over the past century, with the rapid development of the industrial revolution, energy has become the fundamental guarantee for human survival and the driving force for development. However, due to the rapid consumption of fossil energy (coal, oil, natural gas, etc.) and the surge in population, the energy crisis has become increasingly prominent. In addition, a large number of illegally or substandardly discharged wastewater has seriously polluted the water environment of our country. From 2013 to 2015, my country's total wastewater discharge reached 240 billion tons. In 2015, the discharge of chemical oxygen de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F3/00H01M8/16
CPCC02F3/005C02F3/34C02F2305/06H01M8/16Y02E60/50
Inventor 汪美贞黄超民冯华军高雨晨吕宇倩张芸芸陈健沈东升
Owner ZHEJIANG GONGSHANG UNIVERSITY
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