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Method for identifying active components in medicinal plant

A technology of active ingredients and medicinal plants, applied in the field of identification of natural medicinal plants, can solve the problem that the effect of Chinese medicinal materials is not as good as that of natural Chinese medicinal materials

Active Publication Date: 2012-10-24
新乡博凯生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the Chinese herbal medicines are limited by the picking area and picking time, and the effect of artificially cultivated Chinese herbal medicines is mostly not as good as that of natural Chinese herbal medicines

Method used

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  • Method for identifying active components in medicinal plant
  • Method for identifying active components in medicinal plant
  • Method for identifying active components in medicinal plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0110] Embodiment 1: Epimedium medicinal material IGD carbon nuclear magnetic resonance spectrum fingerprint

[0111] The corresponding Chinese name of active ingredient in the present embodiment is:

[0112] Epimedoside C: Icariin C; Acuminatin: Icariin;

[0113] Epimedokoreanoside I: Epimedin I; Desmethylanhydroicaritin: Demethylated dehydro-icaritin;

[0114] Caohuoside A: Picariin A; Caohuoside E: Picariside E;

[0115] Icariin: Icariin; Icariside II: Icariside II;

[0116] Icaritin-3-rhamnoside: Icaritin-3-rhamnosyl flavone;

[0117] 3′″-carbonyl-2″-β-L-quinovosyl icariin: 3′″-carbonyl-2″-β-L-quinoyl icariin;

[0118] 3′″-carbonyl-2″-β-L-quinovosyl icariside II: 3′″-carbonyl-2″-β-L-quinoyl icariside II;

[0119] Epimedin A: Epimedin A; Epimedin B: Epimedin B; Epimedin C:

[0120] Epimedin C; Epimedin K: Epimedin K;

[0121] Sagittatoside A: sagittatoside A: Sagittatoside B: sagittatoside B.

[0122] (1) Preparation of characteristic extracts of medicinal materials...

Embodiment 2

[0168] Embodiment 2: Epimedium extract IGD carbon nuclear magnetic resonance spectrum fingerprint collection (external standard method)

[0169] (1) Preparation of characteristic extracts of medicinal materials

[0170] Epimedium extract products from different manufacturers were selected as characteristic extracts.

[0171] (2) Feature extraction IGD carbon NMR spectrum test

[0172] Method 1: Take Epimedium extract 50mg + Dicoumarolum (External standard, purity 99.8%, molecular formula C 19 h 12 o 6 , molecular weight: 336.31) 5mg, dissolved in 0.5mLDMSO-d 6 In the process, the carbon-1 IGD nuclear magnetic resonance spectrum is made, and the fingerprint of the carbon-1 NMR spectrum of the IGD is obtained.

[0173] Method 2: Take Epimedium extract 55mg+hydroquinone (external standard, purity 99.8%, molecular formula C 6 h 6 o 2 ; molecular weight 110.11) 3mg, dissolved in 0.5mL DMSO-d 6 In the process, the carbon-1 IGD nuclear magnetic resonance spectrum is made, an...

Embodiment 3

[0200] Example 3: IGD carbon nuclear magnetic resonance spectrum fingerprint

[0201] (1) Preparation of the characteristic extract of Donglingcao medicinal material

[0202] 1) Selection of the best extraction and separation procedure (Taking Rubescens 1 commercially available in Henan as an example)

[0203] ① Extraction method 1

[0204] Weigh the crushed aboveground part of Dongling grass, reflux extraction with 95%v ethanol aqueous solution 8 times its weight (mL / g) volume, 2 hours each time; 0.7 times (mL / g) volume, directly added to activated carbon adsorption, after suction filtration, evaporated to dryness for later use. See attached Figure 3-1-1 .

[0205] ②Extraction method 2

[0206] Weigh the crushed aboveground part of Dongling grass, reflux extract twice with 95%v ethanol aqueous solution with 8 times its weight (mL / g) volume, each time for 2h; 1.5-1.7 times (mL / g) volume, add 1v times water solution, let stand for 12 hours, centrifuge, precipitate, dry a...

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Abstract

The invention provides a method for identifying active components in a medicinal plant, comprising the following steps: extracting the medicinal plant to obtain a medicinal plant characteristic extract containing the active components; carrying out IGD C Nuclear magnetic resonance fingerprint detection on the characteristic extract to obtain the peak intensities of characteristic peaks of some active components in characteristic extract the according to the fingerprint; detecting with a same way to obtain the peak intensities of characteristic peaks of corresponding standard references of each active component; conducting quantitative analysis to obtain the absolute contents of the standard references; and calculating the contents of each active component and the content of the active components through a coupling formula by using a ratio of each peak intensity of the characteristic peak of each active component to the peak intensity of the characteristic peak of the corresponding reference and the absolute content. According to the invention, the active components included in the medicinal plant and the ratio among them and the total content can be accurately reflected, thus the purpose of accurate identification of the medicinal plant and quality evaluation of medicinal plant source product are achieved.

Description

technical field [0001] The invention belongs to the field of identification of natural medicinal plants, and in particular relates to a method for identifying active component groups in medicinal plants. Background technique [0002] Fingerprint technology is based on the concept of "fingerprint" in forensic science, and it is proposed for the diversification and complexity of active ingredients in natural products. an important technology. Fingerprints are images, graphs, spectra and data obtained by using modern information collection technology and certain qualitative and quantitative analysis methods that can show the characteristics of natural products [Zhou Yuxin, Chinese Medicine Fingerprint Research Technology [M]. Beijing, Chemical Industry Publishing Society. 2002], it has been recognized internationally. [0003] Fingerprints mainly include biological fingerprints and chemical fingerprints [Luo Guoan et al., Classification and Development of Traditional Chinese ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08
Inventor 赵天增张海艳董建军郭唯范毅
Owner 新乡博凯生物技术有限公司
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