Rapid purification method of apolipoprotein B
A technology of apolipoprotein and purification method, which is applied to the preparation methods of apolipoproteins and peptides, chemical instruments and methods, etc., can solve the problems of complicated operation, prolonged production cycle, low purification efficiency, etc., and achieves simple operation steps and reduced The effect of purification cost and high purification yield
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0021] Example 1 Purification of apolipoprotein B.
[0022] Take 5mL of human plasma, add 5mL of pH 6.0 phosphate buffer to dilute at a volume ratio of 1:1, and mix well to obtain a diluted plasma sample; heat the mixed diluted plasma sample in the first water bath at 70°C, heat During this period, gently mix the diluted plasma sample once every 5 minutes, and after heating for 20 minutes, centrifuge the diluted plasma sample at 8000g for 15 minutes, take the supernatant, and separate the denatured impurity protein precipitation; Add 0.1mL Triton X-100, mix well, let it stand for 4 hours, place it in a second water bath at 55°C for heating, and gently mix the supernatant once every 5 minutes during the heating period, after heating for 20 minutes, Centrifuge the supernatant at 8000 g for 15 minutes, discard the supernatant, and collect the precipitate; wash the collected precipitate twice with 0.05 mol / L Tris-HCl buffer solution, and filter with suction to obtain purified ap...
Embodiment 2
[0024] Example 2 Purification of apolipoprotein B.
[0025] Take 5 mL of human plasma, add 300 μL of 1% D-glucoside sulfate solution, shake and mix. After the color of the serum turns light yellow, add 300 μL of 2 mol / L calcium chloride, mix by inverting repeatedly for 15 minutes, and then put it in a refrigerator at 4°C overnight. Then, centrifuge at 4000g for 30min at 4°C, discard the supernatant, dissolve the precipitate in 5mL of 12% sodium chloride, add pH7.4, 0.2mol / L Tris-HCl buffer 5mL and centrifuge After 10 min, the supernatant was discarded, and the obtained precipitate was washed once with 0.02 mol / L Tris-HCl; this step was repeated 3 times. The precipitate was dissolved in 10 mL of 1mol / L potassium oxalate, and centrifuged at 3000 g for 30 min at 4°C to remove the precipitate. The resulting solution was dialyzed in 1% sodium chloride and 1% barium chloride for 48 hours (4°C), the dialyzed solution was deprecipitated, and the obtained supernatant was put into 0...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap