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Method for preparing xylo-oligosaccharide by corn husk enzymatic hydrolysis

A technology for xylo-oligosaccharides and corn husks is applied in the field of bioengineering, which can solve problems such as high energy consumption, and achieve the effects of simple adsorption operation, improved purity, and convenience for large-scale industrial production.

Inactive Publication Date: 2009-12-09
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Corn bran contains a certain amount of starch and protein, which are interwoven with the three components of cellulose, hemicellulose and lignin. Direct utilization of it cannot achieve the desired effect, so it should be pretreated first. The pretreatment methods at home and abroad mainly include physical methods and chemical methods. The physical methods mainly include mechanical crushing, but this method consumes a lot of energy; the chemical method includes steam explosion crushing. This method has a high conversion rate of materials, but requires special equipment, and the reaction process will produce some microbial inhibitors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for preparing xylooligosaccharides by enzymatic hydrolysis of corn husks, the specific steps are:

[0026] (1) Corn husks are mixed with water according to the material-to-liquid ratio of 1:4, boiled at 110°C for 15 minutes, and added 50U / g 样品 The medium temperature amylase was stirred and hydrolyzed for 2.5h under the condition of 70℃ water bath, and then added 4000U / g 样品 The neutral protease was stirred and hydrolyzed in a water bath at 45° C. for 2.5 hours, and then filtered to obtain the liquefied residue of corn bran. This step can dissolve the starch and protein contained in the corn bran.

[0027] After testing: the starch residue dropped from 13.84% to 2.04%, and the protein residue dropped from 12.5% ​​to 5.1%.

[0028] (2) Add water to the liquefied slag of corn husks to adjust the slurry to a substrate concentration of 7%, adjust the pH to 5.0 with acetic acid buffer, and add 3000U / g respectively 样品 The xylanase and β-glucanase were stirred and hyd...

Embodiment 2

[0034] A method for preparing xylooligosaccharides by enzymatic hydrolysis of corn husks, the specific steps are:

[0035] (1) Corn husks are mixed with water according to the material-to-liquid ratio of 1:8, boiled at 108°C for 20 minutes, and added 100U / g 样品 The medium temperature amylase was stirred and hydrolyzed for 1.5h under the condition of 70℃ water bath, and then added 3000U / g 样品 The neutral protease was stirred and hydrolyzed in a water bath at 45° C. for 2.5 hours, and then filtered to obtain liquefied slag of corn husks. This step dissolved the starch and protein contained in the corn husks.

[0036] After testing: the measured starch residue dropped from 13.84% to 1.28%, and the protein residue dropped from 12.5% ​​to 7.5%.

[0037] (2) Add water to the liquefied slag of corn husk to make a 5% substrate concentration, adjust the pH to 5.0 with acetic acid buffer, add 4000U / g sample xylanase and β-glucanase respectively, at 50°C Stir and hydrolyze for 15 hours i...

Embodiment 3

[0043] A method for preparing xylooligosaccharides by enzymatic hydrolysis of corn husks, the specific steps are:

[0044] (1) Corn husks are mixed with water according to the material-to-liquid ratio of 1:8, boiled at 110°C for 20 minutes, and added 80U / g 样品 The high-temperature amylase was stirred and hydrolyzed in a water bath at 95°C for 2 hours, and then added 2000U / g 样品 The neutral protease was stirred and hydrolyzed in a water bath at 45° C. for 3 hours, and then filtered to obtain liquefied slag of corn husks. This step dissolved the starch and protein contained in the corn husks.

[0045] After testing: the starch residue dropped from 13.84% to 0.864%, and the protein residue dropped from 12.5% ​​to 9.1%.

[0046] (2) Add water to the liquefied slag of corn husks to adjust the slurry to a substrate concentration of 5%, adjust the pH to 5.0 with acetic acid buffer, and add 5000U / g respectively 样品 The xylanase and β-glucanase were stirred and hydrolyzed in a water bat...

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Abstract

The invention relates to a method for preparing xylo-oligosaccharide by corn husk enzymatic hydrolysis. In the method, corn husks are added with water to be in pulp conditioning; after high-temperature boiling for a short time, the mixture is pre-treated by diastasum diastace and protease and filtered; filter residues are added with water to be in pulp conditioning so as to be degraded by xylanase and Beta-glucanase; and after hydrolysate filters ions, xylo-oligosaccharide is obtained by separating a simulated moving bed (SMB). According to the material components, the invention adopts a corresponding compound enzyme targeted hydrolysis macromolecular compound method to produce xylo-oligosaccharide with high additional value; xylo-oligosaccharide liquid contains no furfural compound, improves the purity of hemicellulose, ensures the nutrient component of hydrolyzed substance, and reduces the generation of melanoidin pigment caused by Maillard reaction; in addition, the method of the invention avoids the disadvantages of high requirement for acid hydrolyzer, hard control of hydrolysis speed, mass harmful substance generation and complex refining technology, and conforms to the national requirements of resource saving, and energy saving and emission reduction.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for preparing xylooligosaccharides by enzymatic hydrolysis of corn hulls. Background technique [0002] The corn deep processing industry is one of the important industries in the national economy. Because of its many products, complete functions and close relationship with people's life, it has made great progress in recent years. However, this kind of development is highly dependent on resources and energy. Its outstanding performance The by-products and unused components in the raw materials produced during the deep processing of corn are discharged as waste, causing environmental pollution. [0003] As the main by-product of starch industrial production, corn bran is a kind of cheap plant fiber raw material, which is large and concentrated. At present, the comprehensive utilization of corn bran in China is mainly to mix it with concentrated corn soaking water...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/12C12P19/04C12P19/00
Inventor 路福平王春霞孟娜别松涛张朝正蒋彦洁
Owner TIANJIN UNIV OF SCI & TECH
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