Method for preparing xylo-oligosaccharide by corn husk enzymatic hydrolysis

Abstract

The invention relates to a method for preparing xylo-oligosaccharide by corn husk enzymatic hydrolysis. In the method, corn husks are added with water to be in pulp conditioning; after high-temperature boiling for a short time, the mixture is pre-treated by diastasum diastace and protease and filtered; filter residues are added with water to be in pulp conditioning so as to be degraded by xylanase and Beta-glucanase; and after hydrolysate filters ions, xylo-oligosaccharide is obtained by separating a simulated moving bed (SMB). According to the material components, the invention adopts a corresponding compound enzyme targeted hydrolysis macromolecular compound method to produce xylo-oligosaccharide with high additional value; xylo-oligosaccharide liquid contains no furfural compound, improves the purity of hemicellulose, ensures the nutrient component of hydrolyzed substance, and reduces the generation of melanoidin pigment caused by Maillard reaction; in addition, the method of the invention avoids the disadvantages of high requirement for acid hydrolyzer, hard control of hydrolysis speed, mass harmful substance generation and complex refining technology, and conforms to the national requirements of resource saving, and energy saving and emission reduction.

Description

technical field

[0001] The invention belongs to the technical field of bioengineering, in particular to a method for preparing xylooligosaccharides by enzymatic hydrolysis of corn hulls. Background technique

[0002] The corn deep processing industry is one of the important industries in the national economy. Because of its many products, complete functions and close relationship with people's life, it has made great progress in recent years. However, this kind of development is highly dependent on resources and energy. Its outstanding performance The by-products and unused components in the raw materials produced during the deep processing of corn are discharged as waste, causing environmental pollution.

[0003] As the main by-product of starch industrial production, corn bran is a kind of cheap plant fiber raw material, which is large and concentrated. At present, the comprehensive utilization of corn bran in China is mainly to mix it with concentrated corn soaking water...