Method for preparing xylo-oligosaccharide by corn husk enzymatic hydrolysis
A technology for xylo-oligosaccharides and corn husks is applied in the field of bioengineering, which can solve problems such as high energy consumption, and achieve the effects of simple adsorption operation, improved purity, and convenience for large-scale industrial production.
Examples
Embodiment 1
[0025] A method for preparing xylooligosaccharides by enzymatic hydrolysis of corn husks, the specific steps are:
[0026] (1) Corn husks are mixed with water according to the material-to-liquid ratio of 1:4, boiled at 110°C for 15 minutes, and added 50U / g 样品 The medium temperature amylase was stirred and hydrolyzed for 2.5h under the condition of 70℃ water bath, and then added 4000U / g 样品 The neutral protease was stirred and hydrolyzed in a water bath at 45° C. for 2.5 hours, and then filtered to obtain the liquefied residue of corn bran. This step can dissolve the starch and protein contained in the corn bran.
[0027] After testing: the starch residue dropped from 13.84% to 2.04%, and the protein residue dropped from 12.5% to 5.1%.
[0028] (2) Add water to the liquefied slag of corn husks to adjust the slurry to a substrate concentration of 7%, adjust the pH to 5.0 with acetic acid buffer, and add 3000U / g respectively 样品 The xylanase and β-glucanase were stirred and hyd...
Embodiment 2
[0034] A method for preparing xylooligosaccharides by enzymatic hydrolysis of corn husks, the specific steps are:
[0035] (1) Corn husks are mixed with water according to the material-to-liquid ratio of 1:8, boiled at 108°C for 20 minutes, and added 100U / g 样品 The medium temperature amylase was stirred and hydrolyzed for 1.5h under the condition of 70℃ water bath, and then added 3000U / g 样品 The neutral protease was stirred and hydrolyzed in a water bath at 45° C. for 2.5 hours, and then filtered to obtain liquefied slag of corn husks. This step dissolved the starch and protein contained in the corn husks.
[0036] After testing: the measured starch residue dropped from 13.84% to 1.28%, and the protein residue dropped from 12.5% to 7.5%.
[0037] (2) Add water to the liquefied slag of corn husk to make a 5% substrate concentration, adjust the pH to 5.0 with acetic acid buffer, add 4000U / g sample xylanase and β-glucanase respectively, at 50°C Stir and hydrolyze for 15 hours i...
Embodiment 3
[0043] A method for preparing xylooligosaccharides by enzymatic hydrolysis of corn husks, the specific steps are:
[0044] (1) Corn husks are mixed with water according to the material-to-liquid ratio of 1:8, boiled at 110°C for 20 minutes, and added 80U / g 样品 The high-temperature amylase was stirred and hydrolyzed in a water bath at 95°C for 2 hours, and then added 2000U / g 样品 The neutral protease was stirred and hydrolyzed in a water bath at 45° C. for 3 hours, and then filtered to obtain liquefied slag of corn husks. This step dissolved the starch and protein contained in the corn husks.
[0045] After testing: the starch residue dropped from 13.84% to 0.864%, and the protein residue dropped from 12.5% to 9.1%.
[0046] (2) Add water to the liquefied slag of corn husks to adjust the slurry to a substrate concentration of 5%, adjust the pH to 5.0 with acetic acid buffer, and add 5000U / g respectively 样品 The xylanase and β-glucanase were stirred and hydrolyzed in a water bat...
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Description
Claims
Application Information
- IPC
- C12P19/14; C12P19/12; C12P19/04; C12P19/00
- Inventors
- 路福平; 王春霞