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Application of forsythin to preparation of medicine for improving cognitive function and treating Alzheimer's diseases

A technology for Alzheimer's disease and cognitive improvement, applied in the field of medicine, to achieve good safety, improve health quality and quality of life, and improve cognitive function decline.

Inactive Publication Date: 2012-11-21
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the application of forsythin in the preparation of drugs for improving cognitive function and treating Alzheimer's disease at home and abroad. Therefore, research and development use forsythin to protect brain nerve cells, regulate the secretion of brain neurotransmitters, and improve cognitive function. And the treatment of Alzheimer's disease is extremely valuable

Method used

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  • Application of forsythin to preparation of medicine for improving cognitive function and treating Alzheimer's diseases
  • Application of forsythin to preparation of medicine for improving cognitive function and treating Alzheimer's diseases
  • Application of forsythin to preparation of medicine for improving cognitive function and treating Alzheimer's diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Experimental study on the intervention of forsythin on cognitive impairment in aged mice

[0022] 1.1 Animals

[0023] There were 20 1-month-old ICR mice and 80 8-month-old ICR mice, half male and half male.

[0024] 1.2 Drugs

[0025] Forsythin was purchased from Jiangsu Nanjing Zelang Pharmaceutical Technology Co., Ltd., and the purity detected by HPLC was higher than 98%.

[0026] 1.3 Test equipment

[0027] The composition of Morris water maze: The water maze consists of a circular pool (90cm in diameter, 50cm in height), a platform and a recording system. Arbitrarily divide the pool into four quadrants (northeast, southeast, southwest, and northwest). At the beginning of the experiment, the pool was filled with water to a depth of 30 cm, and 1 catties of milk powder was added to make the water opaque and milky white, and the water temperature was kept at about 24 °C ± 1 °C. There are abundant spatial references (lights, cameras, operators, etc.) aro...

Embodiment 2

[0045] Example 2: Forsythin improves cognitive function in rats with Alzheimer's disease induced by hippocampal injection of Aβ

[0046] 2.1 Animals

[0047] Fifty SD rats (8-10 months old, half male and half male, body weight 380-400 g) were purchased from Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd.

[0048] 2.2 Drugs and Reagents

[0049] Forsythin is the same as Example 1;

[0050] Aβ1-42 was purchased from Sigma-Aldrich Company.

[0051] 2.3 Grouping

[0052] The animals had free access to food and water, the room temperature was 20°C-22°C, and the relative humidity was 60%-70%. / kg), 10 in each group, half male and half female. Oral administration was started 3 days after modeling; behavioral tests were performed 6 weeks later.

[0053] 2.4 The rat model of hippocampal CA1 injection of Aβ

[0054]Rats with normal learning and memory function were screened by platform jumping test. Aβ1-42 dimethyl sulfoxide was dissolved and incubated in a 37°C inc...

Embodiment 3

[0062] Example 3: Effects of forsythin on central monoamine neurotransmitters in the brain of aged mice

[0063] 3.1. Instruments

[0064] HPLC detection system: binary pump, chromatography column, automatic sampler, electrochemical detector.

[0065] 3.2 Drugs and Reagents

[0066] NE, DA, 5-HT, 5-HIAA: purchased from Sigma-Aldrich Company.

[0067] HPLC grade perchloric acid, acetonitrile: Fisher Scientific.

[0068] 3.3 Detection method

[0069] 3.3.1 Specimen preparation

[0070] After the behavioral test in Example 1, the mice were killed by decapitation, and the whole brain was quickly taken out from the ice table, weighed, quickly frozen in liquid nitrogen, and stored at -80°C. During extraction, the whole brain was homogenized in ice-cold 0.1 mol / L perchloric acid for 20 s (1 mL of perchloric acid was added per 0.1 g of brain weight). 0.04% (w / v) Na in perchloric acid 2 S 2 O 5 and 0.04% (w / v) EDTA. Brain homogenate was centrifuged at 14,000 × g for 20 min at...

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Abstract

The invention discloses novel application of forsythin belonging to active components of natural medicinal plants, and particularly relates to application of forsythin as the only active ingredient to preparation of a medicine for improving a cognitive function and treating Alzheimer's diseases. Animal and cell research results indicate that the forsythin has the exact function of improving the cognitive function and can remarkably improve the spatial learning and memory ability of animals in an aging and Alzheimer's disease model, the level of a center monoamine neurotransmitter of an old-aged animal is increased, the genetic expression of presenilin 2 in a rat brain of an Alzheimer's disease model is reduced, and the survival rate of neuroblastoma cells induced by beta-amyloid protein is increased. The forsythin is matched with relevant auxiliary materials, a health care product or a medicine for purposefully improving the cognitive function and treating the Alzheimer's diseases can be prepared by a conventional preparation method, and the health care product and the medicine can be used for delaying and improving relevant diseases of cognitive hypofunction such as the course of the Alzheimer's diseases, enhancing the healthy quality and the life quality of old people and realizing a healthy aging social value.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of a natural medicinal plant active ingredient forsythin in the preparation of a medicine for improving cognitive function and treating Alzheimer's disease. Background technique [0002] Forsythin (CAS No. 487-41-2) is a medicinal plant active ingredient, and its sources include Forsythia suspensa (Thunb.) Vahl, a common Oleaceae plant Chinese medicinal material recorded in the Pharmacopoeia of the People's Republic of China of dried fruits. [0003] With the deepening of population aging, aging cognitive decline and Alzheimer's disease (a neurodegenerative disease, commonly known as senile dementia) have increasingly become one of the serious problems in Chinese society. The factors affecting cognitive function decline in the elderly include both demographic factors (mainly age, education level, etc.), as well as health and disease factors (such as cerebrovascular diseas...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P25/28
Inventor 杨洋
Owner SUZHOU UNIV
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