Bacillus SSAL-6 and application thereof in degrading anabaena flos-aquae

A technology of blooming Anabaena and Bacillus, applied in the field of microorganisms, can solve problems such as rareness and harm to human beings

Inactive Publication Date: 2012-11-28
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Anabaena anabaena (a common algae that causes water blooms) is one of the main algal species that cause eutrophication in water bodies. It is widely distributed and can produce algal toxins, which directly and indirectly harm humans. The study of Anabaena sinensis is very rare

Method used

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  • Bacillus SSAL-6 and application thereof in degrading anabaena flos-aquae
  • Bacillus SSAL-6 and application thereof in degrading anabaena flos-aquae
  • Bacillus SSAL-6 and application thereof in degrading anabaena flos-aquae

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Embodiment 1

[0014] Example 1. Separation, purification and identification of Bacillus SSAL-6

[0015] 1. Isolation and purification of Bacillus SSAL-6

[0016] Taking the yellowed Anabaena algae liquid from the Key Laboratory of Urban Agriculture (South) of the Ministry of Agriculture as the isolation source of algae-lysing bacteria, the 25 strains of bacteria obtained were purified and separated several times by the coating plate method and the partition line method. They were placed in 100 mL LB liquid medium, and the shaking speed was 180 r min. -1 , cultured at 37°C for 24h, and then took 800 μL dropwise and added it to the solid plate of Anabaena algae. The size of the algal plaque was investigated and the algae lytic effect of each bacteria was compared, and finally the strain with higher algae lytic effect was screened out. SSAL-6. It was identified as Bacillus and named Bacillus SSAL-6. It was connected to the slant medium and stored in the refrigerator at 4°C; the bacterial su...

Embodiment 2

[0023] Example 2. The effect of Bacillus SSAL-6 on the growth effect and photosynthetic pigments of Anabaena alba

[0024] 1. The effect of Bacillus SSAL-6 on the growth of Anabaena alba

[0025] Anabaena algae was cultured with reference to the standard method of algal growth inhibition experiment (Ministry of Environmental Protection), using Shuisheng No. 111 nitrogen-free medium, pH 7.5. The culture temperature was 30±2°C, continuous 24h light, and the light intensity was 3000±200lx.

[0026] The concentration of Anabaena in the bloom is 5 x 10 4 ~1×10 5 Bacillus SSAL-6 (the cell concentration is about 10 9 A / mL), the treatment concentration (v / v) gradient is: 5%, 10%, 15%, 20%, 25%, 30% and 35%, and each group of samples has three replicates. A series of culture solutions with different ratios (LB culture solution: Shuisheng No. 111 nitrogen-free culture solution) were prepared as in the experimental group, which were used to cultivate Bacillus SSAL-6 and Anabaena alba...

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Abstract

The invention discloses a Bacillus SSAL-6 and an application thereof in degrading anabaena flos-aquae. The bacillus is obtained by separating and screening yellowed anabaena flos-aquae in Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, is named as Bacillus SSAL-6, and is deposited in China General Microbiological Culture Collection Center with the accession number of CGMCC No.6195 on 6 June 2012. Under natural conditions, the degradation effect of Bacillus SSAL-6 on the anabaena flos-aquae increases with the increase of the strain concentration, when the concentration of the bacillus is 35%, the inhibition rate of anabaena flos-aquae chlorophyll alpha can reach 96%. The Bacillus SSAL-6 disclosed herein has good effect of degrading algae, can effectively degrade the anabaena flos-aquae, provides an effective means for controlling water body eutrophication, and provides an important method for utilizing microbes to treat water bloom.

Description

technical field [0001] The invention relates to a microorganism, in particular to a strain of Bacillus SSAL-6 and its application in degrading Anabaena alba, belonging to the technical field of microorganisms. Background technique [0002] Eutrophication of water bodies has become a universal environmental problem that plagues countries all over the world. The massive reproduction of planktonic algae leads to frequent outbreaks of algal blooms, which seriously affect human life, production and health, and cause ecological disasters worldwide. It is urgent to explore effective ways to control the occurrence of algal blooms. At present, physical and chemical methods are mainly used to control water eutrophication, but these two methods will not only consume a lot of financial and material resources, but also damage the ecological environment to a certain extent. As a control organism for algal blooms and red tides, algae-lysing bacteria have increasingly attracted widespread ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07
Inventor 沈健英孙秀敏
Owner SHANGHAI JIAO TONG UNIV
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