Use of ligustilide in preparing anti-thrombotic medicines

An antithrombotic drug, the technology of Chuanxiong extract, which is applied in the field of preparation of antithrombotic drugs, can solve the problems of unclear mechanism of antithrombotic activity, and achieve the effects of easy quality control, good pharmacological activity, and simple extraction and separation process

Active Publication Date: 2014-04-16
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current research focuses on ligustrazine and ferulic acid, and it is not clear whether ligustralides have antithrombotic activity and their mechanism of action.

Method used

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  • Use of ligustilide in preparing anti-thrombotic medicines
  • Use of ligustilide in preparing anti-thrombotic medicines
  • Use of ligustilide in preparing anti-thrombotic medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment one The preparation of effective component of Rhizoma Chuanxiong

[0036]After crushing 1000g of Rhizoma Chuanxiong medicinal material, add 4000ml of 95% ethanol to soak overnight, reflux extraction for 2 hours, then add 4000ml of 95% ethanol to heat and reflux for extraction three times, each time for 2h, combine the extracts, and concentrate to a density of 1.08. Add the concentrated solution to 500ml of water to disperse into a suspension, add 500ml of petroleum ether to extract three times, combine the extracts, and recover the solvent to dryness. Take petroleum ether to extract part of the extract, add normal phase silica gel to mix the sample, pass through normal phase silica gel column, use petroleum ether and ethyl acetate solution with a volume ratio of 50:1 as the eluent to elute, collect the eluate, and pass through the normal phase silica gel column. After concentration, 11.3 g of light yellow liquid was obtained, which was the Rhizoma Chuanxiong ...

Embodiment 2

[0037] Embodiment two The analysis of the effective components of Rhizoma Chuanxiong

[0038] Weigh and dissolve the Rhizoma Chuanxiong extract as shown in Example 1, and transfer an appropriate amount to a brown liquid phase sampling bottle for liquid chromatography analysis.

[0039] The analysis conditions are as follows: Agilent 1100 liquid chromatograph, injection volume 10 μl; flow rate 1ml / min, column temperature 30°C. The mobile phase gradient for chromatographic analysis is: 0~40min: water (0.1% formic acid): 70%~20%, acetonitrile: 30%~80%; 40-50min: water (0.1% formic acid): 20%~5%, Acetonitrile: 80%~95%; 50~55min: water (0.1% formic acid): 5%, acetonitrile: 95%; detection wavelength 271nm. Analyze the spectrum as figure 1 Shown (the abscissa is minutes). Liquid chromatographic analysis showed that the extract of Chuanxiong Rhizoma had an obvious chromatographic peak at 26.5min, and the compound represented by this peak was identified as ligustilide after compari...

Embodiment 3

[0040] Embodiment three Rhizoma Chuanxiong effective component preparation

[0041] Take 0.5 g of the effective components of Ligusticum chuanxiong obtained in Example 1 and mix evenly with 10.5 g of polyethylene glycol-6000, heat and melt, and after the chemical material is moved to drip irrigation, the drug solution is dropped into 6 ~ 8 ℃ of liquid paraffin, and the Oil, made 400 drop pills.

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Abstract

The invention relates to use of ligustilide in preparing anti-thrombotic medicines, in particular to a rhizoma ligustici wallichii extract. The rhizoma ligustici wallichii extract contains 30-90 percent of ligustilide. The extract is prepared by adopting a method comprising the following steps of: crushing a rhizoma ligustici wallichii medicinal material; then, soaking by using 70-99 percent of alcohol (for example 75-98 percent), and performing reflux extraction for 1-4 times; merging extracting solutions; concentrating; extracting by using petroleum ether; recovering solvents from the merged extracting solutions until dry to obtain petroleum ether extracting extractum; adding part of petroleum ether extracting extractum to positive-phase silica gel and mixing to exceed a positive-phase silica gel column; performing elution by taking the petroleum ether and an ethyl acetate solution as eluents; collecting the eluents; concentrating to obtain the rhizoma ligustici wallichii extract, wherein the volume ratio of the petroleum ether to the ethyl acetate solution is 50:(0.5-5) [for example 50:(0.5-3)].

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine chemistry, and in particular relates to an effective component extracted from Rhizoma Chuanxiong and its preparation method, as well as the use of the component in the preparation of antithrombotic drugs. Background technique [0002] Thrombosis refers to the process in which blood coagulates or certain formed components in blood adhere to each other to form solid masses in the heart and blood vessel cavity. Under pathological conditions, the vascular lumen obstruction and other effects caused by thrombus will cause serious or even fatal harm to the body, and become an important pathogenic factor of cardiovascular and cerebrovascular diseases such as myocardial infarction, stroke, deep vein thrombosis, and pulmonary embolism. [0003] Vascular intimal injury is one of the important causes of thrombus formation. Vascular endothelial cells are a single-cell membrane that separates the s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/236A61K31/365A61P7/02
Inventor 程翼宇张萌王怡
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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