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Application of Lianzhi anti-inflammation dropping pills in preparing medicine for suppressing HT1080 cell proliferation

A technology for HT1080 cell proliferation, applied in the field of application of Lianzhi Xiaoyan Dropping Pills in the preparation of drugs for inhibiting HT1080 cell proliferation

Inactive Publication Date: 2012-12-19
南通市海门江海建设投资有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lianzhi Xiaoyan Dropping Pill is the exclusive product of Hainan Yishun Pharmaceutical Co., Ltd. There is no literature report on its inhibition of fibrosarcoma (HT1080) cell proliferation

Method used

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  • Application of Lianzhi anti-inflammation dropping pills in preparing medicine for suppressing HT1080 cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] Experimental research data of Lianzhi Xiaoyan Dropping Pills inhibiting the proliferation of HT1080 cells

[0008] 1 Experimental materials

[0009] 1.1 Experimental cell lines

[0010] Fibrosarcoma (HT1080), from the laboratory cell bank of our company, was routinely cultured in DMEM+10% FBS.

[0011] 1.2 Experimental drugs

[0012] Research drug: Lianzhi Xiaoyan Dropping Pills, Hainan Yishun Pharmaceutical Co., Ltd., batch number: 20110219

[0013] Liquid storage liquid: Weigh 100mg of the contents of Lianzhi Xiaoyan Dropping Pills, dissolve in 5ml of absolute ethanol, filter through a 0.2μm filter, aliquot into 500μl doff tubes, store at -20°C, and filter absolute ethanol through a 0.2μm filter for future use For the control group.

[0014] 1.3 Experimental reagents

[0015] DMEM (GIBCO Cat.No.12100-061 Lot.No.758137); Fetal Bovine Serum (Zhejiang Tianhang Biotechnology Co., Ltd. Lot.No.100419); NaHCO3 (Shanghai Jiuyi Chemical Reagent Co., Ltd. Cat.No.11810 -03...

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Abstract

The invention discloses application of Lianzhi anti-inflammation dropping pills in preparing a medicine for suppressing HT1080 cell proliferation. The prescription of Lianzhi anti-inflammation dropping pills is common andrographis herb dry extract and screwtree root extract with specifications of 30 pills / bag and 6 bags / box; the dropping pills are taken orally, 30 pills once and 3 times per day; the dropping pills can realize the effects of clearing heat, removing toxin, cooling blood and diminishing swelling, and are used for treating the cough and asthma, mumps, acute tonsillitis and throat impediment and sore throat caused by heat and fire toxin in the lung and stomach; and the dropping pills can suppress HT1080 cell proliferation according to the current discovery.

Description

technical field [0001] The invention relates to a new application of a Chinese patent medicine, in particular to the application of Lianzhi Xiaoyan Dropping Pills in the preparation of medicines for inhibiting HT1080 cell proliferation. Background technique [0002] Fibrosarcoma manifests as a single localized hardened deep nodule, with a tense surface, bright redness, and is not easy to rupture. Usually, the surface skin is normal and can move, but when it invades adjacent tissues, it is fixed and cannot move, and can infiltrate into subcutaneous fat, Muscle, fascia, etc. If the lesion originated from the dermis or later invaded the dermis, the superficial skin may atrophy, pigmentation, and ulceration, and occasionally present as a fungoid mass, and multiple lesions may appear near the local excision scar. After repeated excision, recurrence is still common. Systemic symptoms may appear after multiple relapses. Metastases can be seen in the lungs, occasionally in the li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/19A61P35/00
Inventor 不公告发明人
Owner 南通市海门江海建设投资有限公司