Albumen powder richening in antimicrobial peptide fly maggot, preparation method for albumen powder and feed additive of albumen powder

A feed additive and protein powder technology, applied in animal feed, animal feed, application, etc., to achieve the effect of simple method, increased umami amino acid content, and growth promotion

Active Publication Date: 2013-01-30
河北圣土农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few researches on the development of its products. Lu Jie et al. (Research on the extraction process of antibacterial peptides from housefly maggots, 2007, Acta Entomology) used heating-chromatography to extract antibacterial peptides, and the extraction rate was only 0.26%.

Method used

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  • Albumen powder richening in antimicrobial peptide fly maggot, preparation method for albumen powder and feed additive of albumen powder
  • Albumen powder richening in antimicrobial peptide fly maggot, preparation method for albumen powder and feed additive of albumen powder
  • Albumen powder richening in antimicrobial peptide fly maggot, preparation method for albumen powder and feed additive of albumen powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 is rich in the preparation of antimicrobial peptide fly maggot protein powder and feed additive

[0028] The housefly comes from the Guangdong Provincial Center for Disease Control and Prevention and has been domesticated for a long time. After the adult flies have mated, put them into the egg collection cup (egg trapping material: wheat bran + pig blood + 0.01% ammonia water), collect the fly eggs, and after hatching, transfer to the basic feed (wheat bran + pig blood + yeast) for feeding The larvae developed to the third instar and were induced by ultrasound under the following conditions: 35 kHz, 50 watts for 1 minute, 10 minutes apart, and repeated treatment 3 times; continued feeding for 24 hours, and collected larvae using negative phototaxis; after cleaning and disinfection , homogenate and mash the tissue; filter through 80-mesh gauze, and the filtrate is pretreated at 0 degrees Celsius for 40 minutes to remove surface lipid components; spray-dried ...

Embodiment 2

[0030] The antimicrobial peptide-rich maggot protein powder obtained after the present invention induces and treats housefly larvae with specific ultrasound (35 kilohertz, 50 watts for 1 minute, 10 minutes apart, and repeats the treatment 3 times) is the same as the one without ultrasonic induction. The fly maggot protein powder obtained in the step is respectively filtered through dextran gel, and components with a molecular weight less than 30KD are detected by BCA method, and their antibacterial activity is measured. The results showed that after the induction treatment, the ratio of the components <30KD to the fly maggot protein powder increased from (8.92±0.13) wt% to (21.33±1.22) weight%. The detection method of drug sensitivity test paper was used to test, and it was found that the components with a molecular weight of <30KD had obvious antibacterial effects on Escherichia coli, Staphylococcus aureus, Helicobacter pylori, etc. at a concentration of 40 μg / ml, and the inh...

Embodiment 3

[0031] Example 3 Effects of Feed Additives on Growth Performance and Water Quality of Litopenaeus vannamei in Pollution-free Mariculture

[0032] The fishmeal in group D was replaced with 5% by weight (group A), 10% by weight (group B) and 15% by weight (group C) of feed additives, and group D was the basic feed control group (containing 15% by weight of fishmeal). An 8-week breeding experiment was carried out with the above-mentioned 4 kinds of feeds. Feeds were fed 4 times a day during the experiment. At the end of the breeding experiment, the growth performances such as body length, total length and carapace width were calculated respectively. The growth of body length, total length and carapace width of experimental group C is greater than that of control group D, and the growth of body length, total length and carapace width of experimental group C is greater than that of experimental groups A and B. The survival rate of the prawns in each group of ABCD was 88.0%, 90.3%...

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Abstract

The invention discloses albumen powder richening in antimicrobial peptide fly maggot, a preparation method for the albumen powder and a feed additive of the albumen powder. The albumen powder richening in the antimicrobial peptide fly maggot is prepared by a method comprising the following steps of: (1) feeding flies, luring the flies to lay eggs and feeding larva by using feed after incubation; (2) conducting ultrasound induction on the larva; (3) continuously feeding, collecting the larva, washing, conducting disinfection treatment, and conducting tissue homogenate smashing; and (4) filtering, treating filtrate at 0-5 DEG C, removing surface lipid compositions, drying to obtain the albumen powder; adding auxiliary materials in the albumen powder, pelletizing to obtain the feed additive. A ultrasound stimulation mode under specified conditions is adopted to induce the larva to enable an antimicrobial peptide active ingredient content in a body of a housefly to obviously improve, and the feed additive has certain growth promotion and disease-resistant strengthening effects on litopenaeus vannamei, crucian and the like.

Description

technical field [0001] The invention belongs to the field of preparation of fly maggot protein powder, and relates to a fly maggot protein powder rich in antibacterial peptide, a preparation method thereof and a feed additive. Background technique [0002] With the development of scale and intensification of modern aquaculture industry, people attach great importance to the use of green and pollution-free aquatic feed additives. In the late 1980s, some large cities across the country began to research and develop "pollution-free food". Governments at all levels have formulated corresponding laws and regulations. After my country's accession to the WTO, there has been an upsurge of "pollution-free" and "green" aquatic products in various places. Fishmeal has always been an indispensable high-quality protein source in aquafeeds, but its use has many problems such as high price and large usage. In addition, there are many medication problems in aquaculture, resulting in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/10A23K1/16A01K67/033
Inventor 朱家勇褚夫江金小宝李小波许银叶梅寒芳
Owner 河北圣土农业科技有限公司
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