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A kind of rapid propagation method for industrialized production of dragon bamboo tissue culture seedlings

A technology of tissue culture seedlings and dragon bamboo, which is applied in the field of industrialized production and rapid propagation of dragon bamboo tissue culture seedlings, can solve the problems of not reaching the scale of industrial production, achieve the effects of wide application, reduce pollution rate, and increase induction success rate

Inactive Publication Date: 2016-01-27
YUNNAN HEQI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional ways of raising bamboo seedlings mainly include burying joints, branching bamboo seedlings and branch cuttings, etc., but they are far from meeting the requirements of industrial production scale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Collection and preliminary treatment of explants: collect annual dragon bamboo branches in good growth condition, healthy and free from diseases and insect pests, trim and remove secondary branches and leaves, place them in a refrigerator at 4-8°C for 1-2 days; then press each bamboo Cut the first section into small sections, then cut off the stalk sheaths on the bamboo joints, wipe off the dirt on the surface of the branches and stalks with gauze, and finally wash them with sterile water;

[0025] (2) Carry out three disinfection treatments on the washed dragon bamboo branch section under aseptic conditions: first rinse with sterile water after scrubbing with 75% ethanol of concentration; then put the dragon bamboo branch section into a disinfection bottle with a concentration of 0.1 Carry out the second disinfection in ~1% mercuric chloride aqueous solution for 10-20 minutes. During this process, the disinfection bottle should be shaken continuously so that the dis...

Embodiment 2

[0033] (1) Collection and preliminary treatment of explants: collect annual dragon bamboo branches in good growth condition, healthy and free from diseases and insect pests, trim and remove secondary branches and leaves, place them in a refrigerator at 4-8°C for 1-2 days; then press each bamboo Cut the first section into small sections, then cut off the stalk sheaths on the bamboo joints, wipe off the dirt on the surface of the branches and stalks with gauze, and finally wash them with sterile water;

[0034] (2) Carry out three disinfection treatments on the washed dragon bamboo branch section under aseptic conditions: first rinse with sterile water after scrubbing with 75% ethanol of concentration; then put the dragon bamboo branch section into a disinfection bottle with a concentration of 0.1 Carry out the second disinfection in ~1% mercuric chloride aqueous solution for 10-20 minutes. During this process, the disinfection bottle should be shaken continuously so that the dis...

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Abstract

The invention discloses a rapid propagation method for dendrocalamus giganteus tissue culture seedling industrial production. An annual shoot of dendrocalamus giganteus serves as an explant, after sterilization, the induction culture is conducted through a bud induction culture medium, a lateral bud is obtained, the lateral bud is cut, the multiplication culture is conducted through an enrichment medium, numerous clone plants are obtained, the strong seedling cultivation is conducted, the plants are transferred to a rooting medium, the rooting culture is conducted, and finally complete dendrocalamus giganteus clone plants are obtained. Dendrocalamus giganteus test-tube plantlet greenhouse seedling exercise is conducted through a combination medium, and survival seedlings are subjected to land field culture. By the aid of specific test methods and modified mediums, the problems that during the dendrocalamus giganteus tissue culture process, the sterilization, the multiplication and the rooting are difficult and the genetic stability of test-tube plantlets is poor are solved. A plant tissue culture technology can be used widely in the field of large-scale sympodial bamboo test-tube plantlet industrial production, the technical progress is achieved, and the good basic technology support is provided for bamboo resource development and utilization well.

Description

technical field [0001] The invention relates to a method for tissue culture of Dendrocalamus giganteus, in particular to a rapid propagation method for industrialized production of Dendrocalamus giganteus tissue culture seedlings by using new shoots as explants to induce clustered buds, followed by multiplication and rooting culture. Background technique [0002] The research on tissue culture of bamboo began in 1968 with Alex-ander and Rao on the three-dimensional culture of bamboo hybrid embryos. In 1982, Mehatas et al. reported for the first time that Bambus arundinacea obtained regenerated plants through embryogenic callus. In 2006, Hui Chaomao et al. reported that the rare bamboo species Dendrocalamus sinicus was regenerated through embryogenic callus. So far, tens of kinds of bamboos can obtain complete test-tube seedling plants in a short time by tissue culture. However, the tissue culture technique of bamboo is more difficult compared with other gramineous plants du...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 潘恒张绍智
Owner YUNNAN HEQI BIOTECH CO LTD
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