Asexual rapid propagation method of tea trees and root taking culture solution
A technology for rooting culture and culture solution, applied in the field of rooting culture solution, can solve problems such as land preparation, high management and protection costs, surface vegetation damage, environmental pollution, etc., and achieve the effects of reducing soil erosion, saving management and protection costs, and reducing environmental protection.
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[0026] 1. Prepare rooting medium for cuttings
[0027] Specific formula of cutting rooting culture medium: each liter of culture medium contains NH 4 NO 3 (825mg·L -1 ), KNO 3 (950mg·L -1 ), CaCl 2 2H 2 O (220mg·L -1 ), KH 2 PO 4 (85mg·L -1 ), MgSO 4 ·7H 2 O (185mg·L -1 ), MnSO 4 4H 2 O (22.3mg·L -1 ), ZnSO 4 4H 2 O (8.6 mg·L -1 ),H 3 BO 3 (6.2mg·L -1 ), KI (0.83mg·L -1 ),Na 2 MoO 4 2H2O (0.25 mg L -1 ), CuSO 4 ·5H 2 O (0.025mg·L -1 ), CoCl 2 ·6H 2 O (0.025mg·L -1 ),Na 2 EDTA (37.25mg·L -1 ), FeSO 4 ·7H 2 O (27.85mg·L -1 ), Al 2 (SO 4 ) 3 (10.8mg·L -1 ).
[0028] 2. Prepare growth hormone soaking solution
[0029] Dissolve α-naphthaleneacetic acid (NAA) with a small amount of 95% alcohol, and then dilute to a concentration of 1mg L with distilled water -1 , stored in a 4°C refrigerator until use. Before root soaking of cuttings, according to NAA 100mg·L -1 Growth hormone concentration, add 0.4mg·L -1 Thiamine Hydrochloride (Vitamin ...
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