Breeding method for inducing photinia serrulata somatic cell by using 60Co r ray radiation

A technology of somatic cells and red leaves, applied in the field of plant radiation mutagenesis treatment, to achieve the effect of improving radiation mutagenesis efficiency and broadening germplasm resources

Inactive Publication Date: 2013-02-20
SHANDONG FOREST SCI RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new method allows us to create plants that have improved resistance against harmful chemical agents such as pesticides or herbicide while also being able to produce these crops at higher yields than traditional methods like crop rotation. By utilizing this technique we can improve both efficiency and quality of photosynthetic organisms used for food production.

Problems solved by technology

Technically speaking, this patented technical problem addressed in this patents relates to developing an effective way to improve plants' quality and quantity during commercial vegetations without causing disease damage or harmful insect attacks.

Method used

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  • Breeding method for inducing photinia serrulata somatic cell by using 60Co r ray radiation
  • Breeding method for inducing photinia serrulata somatic cell by using 60Co r ray radiation
  • Breeding method for inducing photinia serrulata somatic cell by using 60Co r ray radiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take the 3rd to 6th unfolded leaves of the test-tube seedlings as explants, the leaves have short petioles, cut off the serrated leaf edges around the leaves, cut the leaves into left and right halves along the main vein, and lightly scratch the leaves once, The back of the leaf is placed close to the medium, and inoculated in the improved MS+2,4-D0.1mg / L+BA0.5mg / L+NAA20.0mg / L+3.0g / L phytagel+20g / L sucrose On wound tissue proliferation medium, pH value 5.8, cultured in dark for 10 days, induced somatic embryogenic callus as radiation material.

[0022] with a radiation dose of 45Gy 60 Cor ray irradiated the above irradiated materials for 60 minutes, and transferred the irradiated materials to the improved MS+2.0mg / L BA+3.5g / L phytagel+20g / L sucrose embryogenic callus differentiation medium, the pH value 5.8, continue dark culture at 25±2°C for 30 days, and subculture once in the middle.

[0023] Transfer the above culture materials to a tissue culture room with a temp...

Embodiment 2

[0027] Take the 3rd to 6th unfolded leaves of the test-tube seedlings as explants, the leaves have short petioles, cut off the serrated leaf edges around the leaves, cut the leaves into left and right halves along the main vein, and lightly scratch the leaves once, The back of the leaf is placed close to the medium, and inoculated in the improved MS+2,4-D0.1mg / L+BA0.5mg / L+NAA20.0mg / L+3.0g / L phytagel+20g / L sucrose On wound tissue proliferation medium, pH value 5.8, cultured in dark for 10 days, induced somatic embryogenic callus as radiation material.

[0028] with a radiation dose of 30Gy 60 Co-r rays irradiated the irradiated material for 90 minutes, and transferred the irradiated material to the improved MS+2.0mg / L BA+3.5g / L phytagel+20g / L sucrose embryogenic callus differentiation medium, pH The value is 5.8, and the temperature is 25°C, continue to culture in dark for 35 days, and subculture once in the middle.

[0029] Transfer the above culture materials to a tissue cu...

Embodiment 3

[0033] Take the 3rd to 6th unfolded leaves of the test-tube seedlings as explants, the leaves have short petioles, cut off the serrated leaf edges around the leaves, cut the leaves into left and right halves along the main vein, and lightly scratch the leaves once, The back of the leaf is placed close to the medium, and inoculated in the improved MS+2,4-D0.1mg / L+BA0.5mg / L+NAA20.0mg / L+3.0g / L phytagel+20g / L sucrose On wound tissue proliferation medium, pH value 5.8, cultured in dark for 10 days, induced somatic embryogenic callus as radiation material.

[0034] with a radiation dose of 15Gy 60 Cor ray irradiated the irradiated material for 120 minutes, and transferred the irradiated material to the modified MS+2.0mg / L BA+3.5g / L phytagel+20g / L sucrose embryogenic callus differentiation medium, pH 5.8 , and then continue dark culture at 25°C for 40 days, and subculture once in the middle.

[0035] Transfer the above culture materials to a tissue culture room with a temperature o...

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Abstract

The invention discloses a breeding method for inducing photinia serrulata somatic cell by using 60Co r ray radiation. The method comprises the steps: processing a photinia serrulata embryonic callus for 1-2 hours by using the 60Co r ray radiation at the irradiation does of 15-45Gy, wherein the photinia serrulata embryonic callus is obtained through dark culture on a callus proliferation culture medium and is taken as a radiation material; after radiation processing, transferring the callus to a callus differentiation culture medium, carrying out dark culture for 30-35 days at the temperature of 25+/-2 DEG C; and finally transferring the callus to a tissue culture room for culture in a light environment, wherein the differential rate of green shoots of the callus is high up to 79%, and a plant is regenerated from the cell screened through radiation induction. The method disclosed by the invention is suitable for breeding new varieties of plants and provides conditions for carrying out plant somatic mutation breeding at a cellular level.

Description

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Claims

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Application Information

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Owner SHANDONG FOREST SCI RES INST
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