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Kit for detecting existence of microorganisms

A kit and microorganism technology, applied in the field of kits for detecting the existence of microorganisms, can solve the problem of taking a long time, and achieve the effect of solving the problem of taking too long

Inactive Publication Date: 2013-03-06
齐开江
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention is to solve the problem that it takes a long time to use a nutrient medium for detecting living cells in the traditional method
[0005] Beneficial effects of the present invention: the present invention is used to amplify and detect traces of exogenous ATP, which works by allowing a fusion protein marker of a polyphosphate kinase and a myokinase and a mixture of a polyphosphate compound to function with It takes too long to solve the problem of traditional methods

Method used

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Embodiment Construction

[0006] A kit for detecting the presence of microorganisms comprising an adenosine triphosphate amplification reagent comprising adenosine monophosphate, a polyphosphate compound and a fusion protein marker having a polyphosphate kinase and a polyphosphate kinase in this order from the N-terminus Myokinase; Adenosine triphosphate detection reagent for the detection of adenosine triphosphate, where the fusion protein marker has been subjected to an apyrase treatment and a pyrophosphate treatment to remove the supposed fusion protein marker adenosine diphosphate, where the fusion protein marker The compound does not exist in the complex with adenosine diphosphate.

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Abstract

The invention discloses a kit for detecting existence of microorganisms. The kit comprises an adenosine triphosphate (ATP) amplifying reagent containing adenylate, a polyphosphate compound and a fusion protein label, wherein a polyphosphate kinase and a myokinase are added from an N end according to the sequence. The ATP detection reagent is used for detecting ATP, wherein the fusion protein label is treated by an apyrase and a pyrophosphate so as to remove adenosine diphosphate of the fusion protein label, and the fusion protein label does not exist in an adenosine diphosphate complex. The kit is used for amplifying and detecting trace of exogenous ATP, plays a role through a mixture of the fusion protein label allowing the polyphosphate kinase and the myokinase and the polyphosphate compound, and is used for solving the problem of overlong time of the traditional method.

Description

technical field [0001] The invention belongs to a kit, in particular to a kit for detecting the presence of microorganisms. Background technique [0002] In the food industry, dairy industry and other industries, it is very important for the rapid detection of microorganisms with high sensitivity, for example, the control of microorganisms in the environment such as the detection of microorganisms in food manufacturing for the prevention of food poisoning Industrial installations are used in the inspection of microbial contamination of food. Using traditional methods to detect viable cells using a nutrient medium has the problem of taking a long time. Contents of the invention [0003] The present invention aims to solve the problem of using a nutrient medium for detecting living cells in the traditional method, which takes a long time. [0004] A kit for detecting the presence of microorganisms comprising an adenosine triphosphate amplification reagent comprising adenos...

Claims

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Application Information

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IPC IPC(8): C12Q1/48C12Q1/04
Inventor 齐开江
Owner 齐开江
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