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Chicken Newcastle disease and avian infectious bronchitis bivalent live vaccine heat-resistant protective agent and preparation process thereof

A technology for chicken infectivity and chicken Newcastle disease, which is applied in the field of heat-resistant protective agents, can solve the problems of long freeze-drying time, affecting the promotion and application of heat-resistant protective agents, and achieves the effects of low cost, low energy consumption, and simple preparation methods

Active Publication Date: 2014-04-16
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these patents all have the problem of long freeze-drying time during use, which affects the promotion and application of heat-resistant protective agents

Method used

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  • Chicken Newcastle disease and avian infectious bronchitis bivalent live vaccine heat-resistant protective agent and preparation process thereof
  • Chicken Newcastle disease and avian infectious bronchitis bivalent live vaccine heat-resistant protective agent and preparation process thereof
  • Chicken Newcastle disease and avian infectious bronchitis bivalent live vaccine heat-resistant protective agent and preparation process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Preparation of heat-resistant freeze-drying protective agent for chicken Newcastle disease and chicken infectious bronchitis

[0022] Recipe 1:

[0023] The composition of liquid A is: 4g of sodium L-glutamate, 5g of sucrose, 2g of D-sorbitol, 2g of sodium thiosulfate, and 0.2g of vitamin C. After completely dissolving, add water for injection to 100mL. Sterilize by filtration through a 0.22 μm filter membrane, and set aside.

[0024] The composition of liquid B is: 4g of polyvinylpyrrolidone, which is completely dissolved and replenished to 100mL with water for injection. Autoclave at 116°C for 30 minutes and set aside.

[0025] Liquid A and liquid B are mixed according to the volume ratio of 1:1, which is the heat-resistant lyoprotectant of formula 1.

[0026] Recipe 2:

[0027] The composition of liquid A is: 5g of sodium L-glutamate, 4g of sucrose, 1g of D-sorbitol, 4g of sodium thiosulfate, and 0.1g of vitamin C. After completely dissolving, add wate...

Embodiment 2

[0034] Example 2 Inoculation of heat-resistant freeze-dried protective agent of chicken Newcastle disease and chicken infectious bronchitis dual live vaccine and experiment of protective efficacy

[0035] 1. Test materials and reagents

[0036] 1.1 strain

[0037] Chicken Newcastle disease virus Lasota strain was purchased from China Veterinary Drug Administration.

[0038] Avian infectious bronchitis virus H120 strain and H52 strain were purchased from China Veterinary Drug Control Institute.

[0039] 1.2SPF eggs were purchased from Beijing Merial Experimental Animal Technology Co., Ltd.

[0040] 1.3 Reagents All reagents of the present invention were purchased from Sigma Company.

[0041] 2. The combination of the dual live vaccine and the freeze-dried protective agent in the test group and the setting of the freeze-drying program

[0042] According to the lyoprotectant of the three formulations of Example 1, they are respectively mixed with Newcastle disease and chick...

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Abstract

The invention discloses a chicken Newcastle disease and avian infectious bronchitis bivalent live vaccine heat-resistant lyophilization protective agent and a preparation process thereof. The heat-resistant lyophilization protective agent is composed of a liquid A and a liquid B according to a volume ratio of 1:1. The liquid A is composed of: 4-6g of L-sodium glutamate, 4-6g of sucrose, 1-3g of D-sorbitol, 2-4g of sodium thiosulfate, 0.1-0.3g of vitamin C, and injection water for complementing the liquid to 100mL. The liquid B is composed of: 4-6g of polyvinyl pyrrolidone, and injection water for complementing the liquid to 100mL. The protective agent provided by the invention has the advantages of simple preparation method, suitability for large-scale production, lower energy consumption, and lower cost. With the protective agent, a lyophilization time is reduced to 28h or shorter, such that application efficiency is greatly improved. The protective agent has good protection performance upon a chicken Newcastle disease and avian infectious bronchitis bivalent live vaccine. With the protective agent, the vaccine can be preserved for mother than 24 months under a temperature of 2-8 DEG C.

Description

technical field [0001] The invention relates to a heat-resistant protective agent for animal virus live vaccines, in particular to a heat-resistant protective agent for chicken Newcastle disease and chicken infectious bronchitis dual live vaccines and a preparation process thereof. Background technique [0002] Lyoprotectant and its preparation process are a key technology in the production of live vaccines, which have a great impact on the storage, transportation and use of vaccines. At present, in my country's veterinary vaccines, live freeze-dried vaccines account for a considerable proportion. The commonly used protective agents in China are mainly milk, sucrose, gelatin, etc., which have simple formulas and poor protective functions for vaccines. Freeze-dried vaccines using these traditional protective agents usually require products to be stored below -15°C, which causes great inconvenience to manufacturers and users, restricts long-term storage and transportation of v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/32A61K39/295A61P31/14A61K39/17A61K39/215A61K47/26A61K47/22A61K47/18A61K47/02
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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