Supercharge Your Innovation With Domain-Expert AI Agents!

Chicken Newcastle disease and avian infectious bronchitis bivalent live vaccine heat-resistant protective agent and preparation process thereof

A technology for chicken infectivity and chicken Newcastle disease, which is applied in the field of heat-resistant protective agents, can solve the problems of long freeze-drying time, affecting the promotion and application of heat-resistant protective agents, and achieves the effects of low cost, low energy consumption, and simple preparation methods

Active Publication Date: 2014-04-16
PU LIKE BIO ENG
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these patents all have the problem of long freeze-drying time during use, which affects the promotion and application of heat-resistant protective agents

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chicken Newcastle disease and avian infectious bronchitis bivalent live vaccine heat-resistant protective agent and preparation process thereof
  • Chicken Newcastle disease and avian infectious bronchitis bivalent live vaccine heat-resistant protective agent and preparation process thereof
  • Chicken Newcastle disease and avian infectious bronchitis bivalent live vaccine heat-resistant protective agent and preparation process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Preparation of heat-resistant freeze-drying protective agent for chicken Newcastle disease and chicken infectious bronchitis

[0022] Recipe 1:

[0023] The composition of liquid A is: 4g of sodium L-glutamate, 5g of sucrose, 2g of D-sorbitol, 2g of sodium thiosulfate, and 0.2g of vitamin C. After completely dissolving, add water for injection to 100mL. Sterilize by filtration through a 0.22 μm filter membrane, and set aside.

[0024] The composition of liquid B is: 4g of polyvinylpyrrolidone, which is completely dissolved and replenished to 100mL with water for injection. Autoclave at 116°C for 30 minutes and set aside.

[0025] Liquid A and liquid B are mixed according to the volume ratio of 1:1, which is the heat-resistant lyoprotectant of formula 1.

[0026] Recipe 2:

[0027] The composition of liquid A is: 5g of sodium L-glutamate, 4g of sucrose, 1g of D-sorbitol, 4g of sodium thiosulfate, and 0.1g of vitamin C. After completely dissolving, add wate...

Embodiment 2

[0034] Example 2 Inoculation of heat-resistant freeze-dried protective agent of chicken Newcastle disease and chicken infectious bronchitis dual live vaccine and experiment of protective efficacy

[0035] 1. Test materials and reagents

[0036] 1.1 strain

[0037] Chicken Newcastle disease virus Lasota strain was purchased from China Veterinary Drug Administration.

[0038] Avian infectious bronchitis virus H120 strain and H52 strain were purchased from China Veterinary Drug Control Institute.

[0039] 1.2SPF eggs were purchased from Beijing Merial Experimental Animal Technology Co., Ltd.

[0040] 1.3 Reagents All reagents of the present invention were purchased from Sigma Company.

[0041] 2. The combination of the dual live vaccine and the freeze-dried protective agent in the test group and the setting of the freeze-drying program

[0042] According to the lyoprotectant of the three formulations of Example 1, they are respectively mixed with Newcastle disease and chick...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a chicken Newcastle disease and avian infectious bronchitis bivalent live vaccine heat-resistant lyophilization protective agent and a preparation process thereof. The heat-resistant lyophilization protective agent is composed of a liquid A and a liquid B according to a volume ratio of 1:1. The liquid A is composed of: 4-6g of L-sodium glutamate, 4-6g of sucrose, 1-3g of D-sorbitol, 2-4g of sodium thiosulfate, 0.1-0.3g of vitamin C, and injection water for complementing the liquid to 100mL. The liquid B is composed of: 4-6g of polyvinyl pyrrolidone, and injection water for complementing the liquid to 100mL. The protective agent provided by the invention has the advantages of simple preparation method, suitability for large-scale production, lower energy consumption, and lower cost. With the protective agent, a lyophilization time is reduced to 28h or shorter, such that application efficiency is greatly improved. The protective agent has good protection performance upon a chicken Newcastle disease and avian infectious bronchitis bivalent live vaccine. With the protective agent, the vaccine can be preserved for mother than 24 months under a temperature of 2-8 DEG C.

Description

technical field [0001] The invention relates to a heat-resistant protective agent for animal virus live vaccines, in particular to a heat-resistant protective agent for chicken Newcastle disease and chicken infectious bronchitis dual live vaccines and a preparation process thereof. Background technique [0002] Lyoprotectant and its preparation process are a key technology in the production of live vaccines, which have a great impact on the storage, transportation and use of vaccines. At present, in my country's veterinary vaccines, live freeze-dried vaccines account for a considerable proportion. The commonly used protective agents in China are mainly milk, sucrose, gelatin, etc., which have simple formulas and poor protective functions for vaccines. Freeze-dried vaccines using these traditional protective agents usually require products to be stored below -15°C, which causes great inconvenience to manufacturers and users, restricts long-term storage and transportation of v...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/32A61K39/295A61P31/14A61K39/17A61K39/215A61K47/26A61K47/22A61K47/18A61K47/02
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com