Method, vaccine composition and kit for exciting specific mucosal immunoreaction

A technology of mucosal immunity and vaccine composition, which is applied in the field of vaccine combination and test kit to stimulate specific mucosal immune response, can solve the problems of difficult mucosal sIgA antibody and HIV mucosal vaccine research progress, etc. The effect of mucosal immunity

Inactive Publication Date: 2013-03-27
NAT CENT FOR AIDSSTD CONTROL & PREVENTION CHINESE CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, research on HIV mucosal vaccines has not progressed smoothly. Although sIgA is the most important antibody in mucosal secretions, it is difficult for conventional HIV vaccines to induce effective mucosal sIgA antibodies after immunization through the reproductive tract, rectum, or nasal cavity. produce [10，11，12]

Method used

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  • Method, vaccine composition and kit for exciting specific mucosal immunoreaction
  • Method, vaccine composition and kit for exciting specific mucosal immunoreaction
  • Method, vaccine composition and kit for exciting specific mucosal immunoreaction

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Material:

[0049] VTKgpe (CGMCC No.1099): AIDS vaccine based on replicating vaccinia virus vector, expressing CN54gag-pol-env protein of HIV Chinese epidemic strain (HIV B / C subtype).

[0050] Recombinant gp140 trimer protein: derived from the CN54 membrane protein of HIV epidemic strain in China (see below).

[0051]Adjuvant: oil-in-water emulsion (ingredients: 0.5% (v / v) Tween 80, 0.5% (v / v) Span 85, 4.3% (v / v) squalene, water for injection, 10nM sodium citrate buffer liquid).

[0052] The immune strategy adopted in the present invention comprises the following steps:

[0053] Purchase 6-8 weeks old, SPF level BALB / c mice, and the primary immunogen is the AIDS vaccine VTKgpe (CGMCC No.1099), the immunization route is intramuscular injection, booster immunization 4 weeks after the initial immunization, the immunogen is the recombinant gp140 trimer constructed in this experiment (see below), the immunization route is intranasal immunization, and a booster immunizati...

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Abstract

The invention relates to a method, a vaccine composition and a kit for exciting specific mucosal immunoreaction. The method, the vaccine composition and the kit provided by the invention can excite efficient specific mucosal immunoreaction for pathogenes such as virus and particularly HIV (human immunodeficiency virus) in an individual.

Description

technical field [0001] The invention relates to the field of immunology, and specifically relates to a method, a vaccine combination and a kit for stimulating specific mucosal immune responses. Background technique [0002] A large number of studies have shown that a large proportion of HIV-infected people are infected through mucosal tissues such as the reproductive tract and rectum [1，2] . If the immune response is established defensively before the viral infection occurs, not only an effective systemic immune response can be induced, but also an efficient mucosal immune effect can be produced, which can effectively play the role of the first line of defense of the mucosal immune system, which is very important for preventing local infection and eliminating the initial infection. The spread of the initial virus in infected cells and the inhibition of virus replication in vivo are of great significance. The main feature of the immune response of the mucosal immune system ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K39/12A61K39/21A61K39/145A61P31/12A61P31/18A61P31/16
Inventor 邵一鸣王书晖刘芳
Owner NAT CENT FOR AIDSSTD CONTROL & PREVENTION CHINESE CENT FOR DISEASE CONTROL & PREVENTION
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