Hematopoietic stem cell cryopreserving method and protective agent

Abstract

The invention discloses a hematopoietic stem cell cryopreserving method and a protective agent. The hematopoietic stem cell cryopreserving method comprises the following specific steps mixing and cryopreserving a hematopoietic stem cell suspension or a marrow fluid and a stem cell protecting agent under a sterile condition, wherein the stem cell protecting agent comprises the following components of DMSO (dimethylsulfoxide), a 1640 sterile medium and a blood preserving solution I in a volume ratio of (9-10): 5: (5-6). By the hematopoietic stem cell cryopreserving method, the cost of the hematopoietic stem cell protecting agent can be reduced; high-cost equipment such as a programmed cooling instrument, and a complicated and high-risk procedure of adding liquid nitrogen are not required; and the hematopoietic stem cell cryopreserving method is easy to operate and easy to popularize in medical institutions at all levels.

Description

Technical field [0001] The present invention relates to a method and a protective agent for cryopreservation of hematopoietic stem cells, in particular to the composition, concentration, temperature and freezing procedures of the hematopoietic stem cell protective agent formula. Background technique [0002] Recent studies have confirmed that autologous hematopoietic stem cell transplantation supports high-dose chemotherapy and can improve the efficacy of chemotherapy-sensitive tumors; allogeneic hematopoietic stem cell transplantation can radically cure leukemia and other malignant hematopoietic diseases. As a safe and effective method, the transplantation of hematopoietic stem cells is increasingly accepted by the majority of medical workers and patients. This is inseparable from the reasonable freezing and resuscitation of hematopoietic stem cells in vitro. The success of hematopoietic stem cell transplantation depends on the freezing of hematopoietic stem cells as well as rap...

AI Technical Summary

Problems solved by technology

The previous classic cryopreservation method required the use of 10% dimethyl sulfoxide (DMSO), 6% hydroxyethyl starch (HES) and 4% human serum albumin (HSA) as hematopoietic stem cell protective agents. , put it into the program cooling device to gradually cool down, then transfer to a deep low temperature refrigerator at -80°C, and finally store it in liquid nitrogen at -196°C. The stem cells preserved under this condition last for a long time, which can reach decades, and the cryopreservation effect of hematopoietic stem cells Good, but there are some problems: 1. The cost of this method is high, the price of human serum albumin is high, and the supply is limited; The center can only be completed under the guidance of experienced operators, and it is difficult to promote; 3. The liquid nitrogen tank for storage has less space and needs to be continuously replenished with liquid nitrogen. The operation is cumbersome and with the extension of storage time, the storage cost is gradually increasing. Increase

These unfavorable factors restrict the clinical promotion of hematopoietic stem cell transplantation

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