Stevia rebaudiana enzyme VI and method for converting rebaudioside-A into rebaudioside-D

A technology of stevia enzyme and stevia, applied in the direction of transferase, fermentation, etc.

Active Publication Date: 2015-07-08
SICHUAN INGIA BIOSYNTHETIC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] So far, there is no report on the production of RD by enzymatic biotransformation, ultrafiltration and other technical methods using RA as raw material

Method used

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  • Stevia rebaudiana enzyme VI and method for converting rebaudioside-A into rebaudioside-D
  • Stevia rebaudiana enzyme VI and method for converting rebaudioside-A into rebaudioside-D
  • Stevia rebaudiana enzyme VI and method for converting rebaudioside-A into rebaudioside-D

Examples

Experimental program
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Effect test

Embodiment 4

[0054] Example 4 Investigating the influence of filtration and concentration on the RD content of solids

[0055] After obtaining the reaction solution according to the conversion method of Examples 1-18, if filter paper is used and dried, solid substance A is obtained; if 10kD ultrafiltration membrane is used for ultrafiltration, the permeate is dried to obtain solid substance B; The ultrafiltrate was recrystallized and dried to obtain the solid substance C. The RD was detected by the HPLC detection method in the national standard GB8270-1999. The results are shown in Table 5.

[0056] table 5

[0057]

[0058] It can be seen from Table 5 that the content of rebaudioside D in the solid A can reach at least 60%, and the RD of the solid C red after further purification can reach 80%, or even greater than 90%.

[0059] In summary, using the above-mentioned enzyme catalysis and separation method, the conversion rate of rebaudioside D obtained from rebaudioside A as raw material is ≥70%, ...

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Abstract

The invention belongs to the field of food chemistry, and particularly relates to a method for converting RA (rebaudioside-A) which is taken as a raw material into RD (rebaudioside-D), and the key is stevia rebaudiana enzyme VI and a preparation method thereof. The converting method comprises the following steps: A, the RA of which the purity is higher than 90% is dissolved with water according to the weight measurement ratio of 1:6-1:10m / v; B, UDP-Glu (Uridine Diphosphate Glucose) is added according to the weight ratio that RA:UDP-Glu=15:5-15:9; C, the stevia rebaudiana enzyme VI is added according to the weight ratio that RA: stevia rebaudiana enzyme VI=(800-1200):1 (preferably 1000:1), and then reaction is carried out for 3-7h at the temperature of 40-60 DEG C and the pH of 6-8; and D, a reaction solution is filtered and dried to obtain RD-containing solid matter. The conversion rate of converting RA into RD is larger than or equal to 70%, and the RD content in the solid matter is 60%-70%, can achieve 80% after further separation and purification, and even can be larger than 90%.

Description

Technical field [0001] The present invention relates to the field of food and chemical industry, in particular to a method for converting rebaudioside A into rebaudioside D using rebaudioside A as a raw material. The key lies in the catalyst stevia enzyme VI and a preparation method thereof. Background technique [0002] Steviol glycosides are a general term for stevia extracts. Its sweet components include Stevioside, Rebaudioside A, B, C, D, E (ebaudioside A / B / C / D / E), and Stevia Steviolbioside, Dulcoside A and so on. Stevia is originally produced in South America and has been used for hundreds of years. The characteristics of high sweetness and low calories of steviol glycosides are just in line with the needs of modern life. The Ministry of Health of my country (85) Weifangzi No. 37 on June 5, 1985 approved steviol glycosides as new food additives. The Joint FAO / WHO Expert Committee on Food Additvies (JECFA) has also confirmed its safety. [0003] However, traditional stevio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/10C12P19/56
Inventor 李沁桐华君欧阳劲秦岭
Owner SICHUAN INGIA BIOSYNTHETIC CO LTD
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