4 results about "Uridine diphosphate glucose" patented technology

The application relates to the field of biological medicine, and discloses a glucosyltransferase mutant, a coding gene of the glucosyltransferase mutant, a recombination carrier, a recombination strain, an enzyme preparation, and application of the glucosyltransferase mutant. The glucosyltransferase mutant is a point mutation at the R position of the amino acid sequence shown in SEQ ID NO:1, and 4-8 histidines are added at the N end. A method for preparing gastrodin comprises the following steps: contacting the glucosyltransferase mutant with p-hydroxybenzyl alcohol and uridine diphosphate glucose. The glucosyltransferase mutant provided by the application has higher specificity for the substrate p-hydroxybenzyl alcohol, thereby obviously improving the conversion rate of the conversion of p-hydroxybenzyl alcohol into gastrodin, and having a good industrial application prospect.

This invention discloses a method for extracting mogroside V, relating to the field of biotechnology. The method involves culturing, fermenting, centrifuging, and homogenizing *E. coli* containing glycosyltransferases UGT-MS1 and UGT-MS2 to obtain an enzyme supernatant. Then, mogroside IIE, uridine diphosphate glucose, the enzyme supernatant, and salt are added to a phosphate buffer solution for enzymatic catalysis to obtain a conversion solution. Finally, the solution is ultrafiltered, loaded onto a column, eluted, and then passed through an alkaline anion exchange resin. The effluent is concentrated and dried to obtain mogroside V. The product has a high yield and is environmentally friendly.

The invention relates to a method for preparing a C-7-position glycoside compound by using an isoflavone aglycone compound, and belongs to the technical field of biology. According to the method, an isoflavone aglycone compound is taken as a substrate, a belamcanda chinensis glycosyl transferase gene BcUGT009 gene encoding protein, a belamcanda chinensis glycosyl transferase BcUGT032 gene encoding protein or a belamcanda chinensis glycosyl transferase BcUGT124 gene encoding protein is taken as a catalyst, uridine diphosphate glucose is taken as a glycosyl donor, and C-7-site glycosylation reaction is performed to generate the corresponding glycoside compound. According to the application, the functions of the three glycosyl transferase genes BcUGT009, BcUGT032 and BcUGT124 in the blackberry lily are identified and verified for the first time, the effects of the three glycosyl transferase genes in catalyzing flavonoid aglycones to generate C-7 glucoside are defined, the blank of research on a specific flavonoid glycoside biosynthesis pathway of the blackberry lily is filled, and important information is provided for research on a biosynthesis mechanism of active ingredients of medicinal plants.

The invention discloses a recombinant uridine diphosphate glucose dehydrogenase mutant and a preparation method thereof, and relates to the technical field of biology, the mutant is derived from pyogenic streptococcus and is obtained through site-specific mutagenesis (mutation site L102V-H129K-I270G), double tags of His-tag and SUMO-tag are fused at the N terminal, the corresponding protein sequence is SEQIDNO.1, and the coding gene sequence is SEQIDNO.2. Compared with a recombinant wild type UGD, the mutant has the advantages that the mutagenesis efficiency is high, and the mutagenesis efficiency is high. The performance of the mutant is remarkably improved, the catalytic activity reaches 1.8 times of that of a wild type, the residual activity is 69.4% after the mutant is stored at room temperature for 5 days, the soluble expression quantity is improved by 5 times, UDPG can be efficiently promoted to be oxidized to generate UDPGA, the commercial production cost of UDPGA is effectively reduced, and the mutant has a wide application prospect. The invention is suitable for the fields of scientific research reagents, biological medicines, cosmetics and the like.