Method for preparing uridine diphosphate glucose by means of immobilized enzyme method
A technology of uridine diphosphate, immobilized enzyme, applied in biochemical equipment and methods, immobilized on or in inorganic carriers, immobilized on/in organic carriers, etc., can solve problems affecting practical application value, Inability to effectively recycle, rapid decline in enzyme activity, etc., to achieve the effects of stable product quality, avoidance of impurities, and easy reaction
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Embodiment 1
[0018] A method for preparing uridine diphosphate glucose by an immobilized enzyme method, specifically comprising the following steps:
[0019] (1) Preparation of UDPG production enzyme: UDPG production enzyme is immobilized on an immobilized carrier, using uridine diphosphate glucose pyrophosphorylase (EC 2.7.7.9, UGP), sucrose phosphorylase (EC 2.4.1.7, SPA) Two enzymatic reactions can be synthesized to prepare immobilized UDPG production enzyme;
[0020] (2) Immobilized UDPG production enzyme: use immobilized UDPG production enzyme to catalyze the preparation of uridine diphosphate glucose reaction liquid, filter and collect the carrier to obtain immobilized UDPG production enzyme;
[0021] (3) Separation of products: the above-mentioned immobilized UDPG production enzyme is directly separated in the reaction tank, and the separated reaction solution is recovered through a filter bag to recover the immobilized UDPG production enzyme, and the permeate is separated by chroma...
Embodiment 2
[0026] A method for preparing uridine diphosphate glucose by an immobilized enzyme method, specifically comprising the following steps:
[0027] (1) Preparation of UDPG production enzyme: UDPG production enzyme is immobilized on an immobilized carrier, using uridine diphosphate glucose pyrophosphorylase (EC 2.7.7.9, UGP), sucrose phosphorylase (EC 2.4.1.7, SPA) Two enzymatic reactions can be synthesized to prepare immobilized UDPG production enzyme;
[0028] (2) Immobilized UDPG production enzyme: use immobilized UDPG production enzyme to catalyze the preparation of uridine diphosphate glucose reaction liquid, filter and collect the carrier to obtain immobilized UDPG production enzyme;
[0029] (3) Separation of products: the above-mentioned immobilized UDPG production enzyme is directly separated in the reaction tank, and the separated reaction solution is recovered through a filter bag to recover the immobilized UDPG production enzyme, and the permeate is separated by chroma...
Embodiment 3
[0034] A method for preparing uridine diphosphate glucose by an immobilized enzyme method, specifically comprising the following steps:
[0035] (1) Preparation of UDPG production enzyme: UDPG production enzyme is immobilized on an immobilized carrier, using uridine diphosphate glucose pyrophosphorylase (EC 2.7.7.9, UGP), sucrose phosphorylase (EC 2.4.1.7, SPA) Two enzymatic reactions can be synthesized to prepare immobilized UDPG production enzyme;
[0036](2) Immobilized UDPG production enzyme: use immobilized UDPG production enzyme to catalyze the preparation of uridine diphosphate glucose reaction liquid, filter and collect the carrier to obtain immobilized UDPG production enzyme;
[0037] (3) Separation of products: the above-mentioned immobilized UDPG production enzyme is directly separated in the reaction tank, and the separated reaction solution is recovered through a filter bag to recover the immobilized UDPG production enzyme, and the permeate is separated by chromat...
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