Method for generating rebaudioside M by catalyzing rebaudioside A via immobilized glycosyltransferases
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0024] The source of the UGT2 gene involved in this experiment is stevia, and the source of the UGT1 gene is rice. The sequence identification numbers are shown in Table 1.
[0025] Table 1 Sequence Identification Number
[0026] name serial identification number illustrate Glycosyltransferase gene UGT2 SEQ ID NO.1 nucleic acid Glycosyltransferase UGT2 SEQ ID NO.2 amino acid Glycosyltransferase gene UGT1 SEQ ID NO.3 nucleic acid Glycosyltransferase UGT1 SEQ ID NO.4 amino acid
[0027] 1. Preparation of engineered bacteria containing target gene
[0028] Utilize the plasmid pET-28a(+) to construct the recombinant plasmid pET-28a(+)-UGT2; use pET-28a(+) as the carrier, and the UGT2 gene base sequence (as shown in SEQ ID NO.1) as the target fragment, after Optimizing digestion, recovery, purification and ligation, constructing expression plasmids, transforming the ligation reaction product into E.coli BL21, and obtaining engi...
Embodiment 2
[0048] The chitosan bead after activation is made with following method:
[0049] (1) dissolve chitosan powder with the acetic acid aqueous solution of volume concentration 2%, be heated to 50 ℃, ultrasonic until the solution is uniform and transparent, obtain the chitosan solution of 40g / L;
[0050] (2) said chitosan solution is dripped into concentration and is in 4mM NaOH aqueous solution, leaves standstill, washes, and obtains the chitosan bead that diameter is 0.1-3.5mm;
[0051] (3) According to the ratio of 1g:30mL, the chitosan pellets were mixed with the glutaraldehyde aqueous solution with a volume concentration of 0.1%, and activated by cross-linking to obtain activated chitosan pellets.
Embodiment 3
[0053] The chitosan bead after activation is made with following method:
[0054] (1) dissolving the chitosan powder with an aqueous acetic acid solution with a volume concentration of 1%, heating to 40° C., and ultrasonically until the solution is uniform and transparent to obtain a 20 g / L chitosan solution;
[0055] (2) the chitosan solution is dripped into a 3mM NaOH aqueous solution, left to stand, and washed to obtain chitosan pellets with a diameter of 0.1-3.5mm;
[0056] (3) According to the ratio of 1g:20mL, the chitosan pellets were mixed with the glutaraldehyde aqueous solution with a volume concentration of 0.01%, and activated by cross-linking to obtain activated chitosan pellets.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com