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Application of non-steroidal anti-inflammatory medicines in preparation of medicine for preventing and treating diabetic encephalopathy

A technology of diabetic encephalopathy and anti-inflammatory drugs, which is applied in the application field of non-steroidal anti-inflammatory drugs in the preparation of drugs for the prevention and treatment of diabetic encephalopathy, which can solve problems such as no literature reports on the treatment of diabetic encephalopathy, and achieve inhibition of protein glycation Mediated damage, increased protein expression and mRNA levels, potent effects

Active Publication Date: 2013-04-17
诺为泰医药科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Some non-steroidal anti-inflammatory drugs (NSAIDs) such as ibuprofen (IB), indomethacin, etc., have antipyretic, analgesic, anti-inflammatory and improve vascular lesions, etc. activity, but there is no literature report on the treatment of diabetic encephalopathy

Method used

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  • Application of non-steroidal anti-inflammatory medicines in preparation of medicine for preventing and treating diabetic encephalopathy
  • Application of non-steroidal anti-inflammatory medicines in preparation of medicine for preventing and treating diabetic encephalopathy
  • Application of non-steroidal anti-inflammatory medicines in preparation of medicine for preventing and treating diabetic encephalopathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: The impact of ibuprofen on the cognitive function of DM rats

[0024] 1 Materials and methods

[0025] 1.1 Experimental animals

[0026] Male SD strain rats, 8-9 weeks old, 190-220g, were provided by the Experimental Animal Center of Xuzhou Medical College.

[0027] 1.2 Drugs and reagents

[0028] Ibuprofen was purchased from Wuhan Huayida Technology Co., Ltd. (purity>99%), STZ was purchased from Sigma-Aldrich Company of the United States, and the glucose test box was purchased from Nanjing Jiancheng Bioengineering Institute.

[0029] 1.3 Method

[0030] 1.3.1 Model establishment Male SD rats were fasted for more than 12 hours, and STZ (dissolved in 0.1 mol / L sodium citrate buffer, pH 4.4 before use) was injected intraperitoneally at 60 mg / kg to establish the model. Seven days after modeling, about 0.2 ml of blood was collected from the orbital venous plexus, centrifuged at 25°C and 3000 rpm for 10 min, and the serum was separated. Non-fasting blood...

Embodiment 2

[0054] Example 2: Effect of ibuprofen on PPARγ expression and mRNA level in the brain of DM rats

[0055] 1 Materials and methods

[0056] 1.1 Experimental animals Same as Example 1.

[0057] 1.2 Drugs and reagents Rabbit anti-PPARγ antibody and rabbit anti-GAPDH antibody were purchased from American Bioworld Company, alkaline phosphatase secondary antibody, phenylmethylsulfonyl fluoride (PMSF) and BCA protein test kit were purchased from Jiangsu Biyuntian Biotechnology Company, Na 3 VO 4 and NaF were purchased from Sinopharm Group (Shanghai) Chemical Reagent Co., Ltd. Rat Ppar-γ and β-actin primers were synthesized by Shanghai Sangon Bioengineering Company, TRIzol was purchased from Invitrogen China Company, and the cDNA synthesis kit was purchased from ABI Company of the United States. Nuclease-free water and 2×Master Mix were purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd., and DNA ladder was purchased from Fermentas Biotechnology Company.

[0058] 1....

Embodiment 3

[0085] Example 3: Effects of ibuprofen on apoptosis and necrosis of hippocampal neurons in the brain of DM rats

[0086] 1 Materials and methods

[0087] 1.1 Experimental animals Same as Example 1.

[0088] 1.2 Drugs and reagents Conventional HE staining reagents were provided by the Department of Pathology, Xuzhou Medical College.

[0089] 1.3 Method

[0090] The apoptosis and necrosis of hippocampal neurons in the brain of DM rats were observed under light microscope by HE staining. The preserved wax blocks were cut into 3 μm thick sections for routine HE staining. HE staining of rat brain sections was commissioned to the Department of Pathology, Xuzhou Medical College.

[0091] 2 results

[0092] The results of HE staining in the CA1 region of the rat hippocampus showed that in the normal group: 3-5 layers of pyramidal cells neatly arranged were observed, the cell shape was normal, and the nucleus was in the middle; in the DM group: the number of pyramidal cells was ...

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Abstract

The invention provides novel pharmacologic action of non-steroidal anti-in-flammatory medicines, i.e. the action for preventing and treating the diabetic encephalopathy. The non-steroidal anti-inflammatory medicines (ibuprofen) can be used for obviously improving the injury of the cognitive function caused by the diabetes mellitus, obviously increasing the protein expression and the mRNA (messenger ribose nucleic acid) level of intracerebral peroxisome proliferators-activated receptor gamma (PPAR gamma), obviously reducing the activity, the protein expression and the mRNA level of cerebral cortex beta amyloid precursor protein gamma-beta amyloid cleaving enzyme 1 (beta amyloid cleaving enzyme 1), the level of end-stage advanrced glycation end products (AGEs), and the protein expression of cyclo-oxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS), and obviously increasing the level of superoxide dismutase (SOD) reduced glutathione (GSH) in blood serum, etc. The non-steroidal anti-in-flammatory medicines can be used for preventing and treating the diabetic complication, i.e. the diabetic encephalopathy.

Description

technical field [0001] The invention relates to a new application of non-steroidal anti-inflammatory drugs, in particular to the application of non-steroidal anti-inflammatory drugs in the prevention and treatment of diabetic encephalopathy. Background technique [0002] Diabetic encephalopathy (Diabetic encephalopathy, DE) refers to the gradual occurrence of long-term diabetes mellitus (DM) patients with mental disorders characterized by mental decline and brain neurophysiological and structural changes. Most common complication. In the state of diabetes, the structural changes of the brain are mainly the cerebral cortex and cortical atrophy, and the functional changes are mainly the impairment of cognitive function. A large number of research reports have confirmed the fact that diabetes causes cognitive dysfunction. In 2006, the introduction of the term diabetes-associated cognitive decline (DACD) further enhanced people's understanding of this dysfunction and became the...

Claims

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Application Information

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IPC IPC(8): A61K31/192A61P3/10A61P25/28
Inventor 印晓星刘耀武朱霞鲁茜张帆
Owner 诺为泰医药科技(上海)有限公司
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