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Raw milk somatic cell detection cuvette and preparation method thereof

A cuvette and somatic cell technology, which is applied in the direction of chemical reaction of materials and material analysis by observing the influence of chemical indicators, can solve the problem of inability to accurately weigh medicines and formulate diazonium salts. Cumbersome and other problems, to achieve the effect of fast detection, low cost and wide application

Active Publication Date: 2013-04-17
INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the esterase method has not been widely used in the acceptance of raw milk and the determination of mastitis in dairy cows. The main reasons are: the amount of aromatic esters used in the detection of raw milk is small and requires precise weighing; the preparation method of diazonium salts is cumbersome and widely distributed The conditions for precise weighing of medicines and preparation of reaction solutions are not available on site

Method used

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  • Raw milk somatic cell detection cuvette and preparation method thereof
  • Raw milk somatic cell detection cuvette and preparation method thereof
  • Raw milk somatic cell detection cuvette and preparation method thereof

Examples

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Embodiment 1

[0039] A raw bovine milk somatic cell detection cuvette, the bottom of the cuvette is provided with a detection block, and the detection block includes a core material and a wall material;

[0040] The composition of the core material includes 1 part of pyrrole ester, 1 part of 1-amino-2-naphthol-4-sulfonic acid diazonium salt, 0.1 part of solubilizing and sensitizing agent, and 200 parts of water to dissolve the above components at a concentration of 0.1 mol / L Tris-hydrochloric acid buffer solution adjusts the pH of the solution to be 8.0;

[0041] The composition of the wall material includes 5 parts of casein, 15 parts of soybean protein and 20 parts of lactose.

[0042] A method for preparing a raw bovine milk somatic cell detection cuvette, said method comprising the following steps:

[0043] 1) Treatment of cuvettes: 751-type plexiglass cuvettes were ultrasonically treated for 10 minutes, rinsed with water, and dried in a hot air oven;

[0044] 2) Core material solutio...

Embodiment 2

[0050] A raw bovine milk somatic cell detection cuvette, the bottom of the cuvette is provided with a detection block, and the detection block includes a core material and a wall material;

[0051] The composition of the core material includes 12 parts of pyrrole ester, 18 parts of 1-amino-2-naphthol-4-sulfonic acid diazonium salt, 1.6 parts of solubilizing and sensitizing agent, and 200 parts of water to dissolve the above components at a concentration of 0.1 mol / L Tris-hydrochloric acid buffer solution adjusts the pH of the solution to be 8.0;

[0052] The composition of the wall material includes 10 parts of casein, 30 parts of whey protein and 50 parts of sucrose.

[0053] A method for preparing a raw bovine milk somatic cell detection cuvette, said method comprising the following steps:

[0054] 1) Treatment of cuvettes: 751-type plexiglass cuvettes were ultrasonically treated for 15 minutes, rinsed with water, and dried in a hot air oven;

[0055] 2) Core material solu...

Embodiment 3

[0061] A raw bovine milk somatic cell detection cuvette, the bottom of the cuvette is provided with a detection block, and the detection block includes a core material and a wall material;

[0062] The composition of the core material includes 8 parts of indole ester, 12 parts of diazonium fluoroborate, 1.0 part of solubilizing and sensitizing agent, 200 parts of water to dissolve the above components, and the concentration is 0.1mol / L Tris-hydrochloric acid buffer solution to adjust the solution pH of 8.0;

[0063] The composition of the wall material includes 8 parts of casein, 20 parts of whey protein and 35 parts of sucrose.

[0064] A method for preparing a raw bovine milk somatic cell detection cuvette, said method comprising the following steps:

[0065] 1) Treatment of cuvettes: 751-type plexiglass cuvettes were ultrasonically treated for 30 minutes, rinsed with water, and dried in a hot air oven;

[0066] 2) Core material solution: 8 parts of indole ester, 12 parts ...

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Abstract

The invention relates to a raw milk somatic cell detection cuvette and a preparation method thereof. A detection block is arranged at the bottom of the cuvette and comprises a core material and a wall material; the core material comprises the following components in part by weight: 1-12 parts of aromatic ester, 1-18 parts of diazonium salt and 0.1-1.6 parts of solubilization and sensitization agent, the components are dissolved with water, and the pH of solution is adjusted to be 8.0 by using buffer solution with a concentration of 0.1 mol / L to obtain core material solution; and the wall material comprises the following components in part by weight: 5-10 parts of casein, 15-30 parts of soy protein or whey protein and 20-50 parts of lactose or cane sugar, and the components are dissolved with water to obtain wall material solution. The preparation method comprises the following steps of: mixing the core material solution with the wall material solution according to 1:1, taking and injecting 0.15-0.25 mL of mixed solution into the cuvette, sealing with a film, pricking a hole in the film and obtaining raw milk somatic cell detection cuvette through freezing, drying and light-shielding. The raw milk somatic cell detection cuvette and the preparation method thereof have the advantage that somatic cells in raw milk can be quickly and conveniently detected.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a raw milk somatic cell detection cuvette and a manufacturing method thereof. Background technique [0002] The somatic cell count in raw milk refers to the total number of cells per milliliter of raw milk. The somatic cell count is an important indicator to measure the quality of raw milk and the health of the mammary glands of supplying raw milk animals. Simple, low-cost, and high-accuracy detection methods are needed. [0003] At present, there are many detection methods for somatic cell count or mastitis in raw milk used in the dairy industry at home and abroad. Common methods include arbitration method-microscopic direct observation method, electronic counter detection method, California cell number measurement method (CMT), esterase method . Among them, the arbitration method-microscopic direct observation method requires staining, film preparation, observation, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 富鑫
Owner INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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