Application of stenotrophomonas rhizophila and cyclic dipeptide metabolite thereof

An oligotrophic, monocytic technology, applied in the direction of microorganism-based methods, microorganisms, fungi, etc.

Inactive Publication Date: 2013-05-08
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Our study found that Stenotrophomonas rhizophila in the soil can induce the model species of nematode fungi-Arthrophyllum oligospora and a variety of nematode fungi to rapidly form a large number of predators under the action of its metabolites Organs, this type of research has not been reported in open literature

Method used

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  • Application of stenotrophomonas rhizophila and cyclic dipeptide metabolite thereof
  • Application of stenotrophomonas rhizophila and cyclic dipeptide metabolite thereof
  • Application of stenotrophomonas rhizophila and cyclic dipeptide metabolite thereof

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Embodiment 1

[0037] The present invention isolates Stenotrophomonas rhizophilus and its cyclic dipeptide metabolites from soil to induce Arthropodium oligosporum to form predatory organs:

[0038] 1. Preparation of AO Sr : Arthropodium oligospora was inoculated in liquid CMB, and cultured at 28° C. for 7 days. The formula of the CMB medium was: corn flour: 20 grams, 1000 milliliters of water, and the pH was natural. Stenotrophomonas rootophilus was cultured at 28° C. for 48 hours with a liquid medium, the formula of which was: peptone 5 g, beef extract 3 g, NaCl 5 g, distilled water 1000 ml, pH 7.2. Take 0.1ml or 1ml of the fermentation broth of Stenotrophomonas rhizophilus and put it into Arthrobuspora oligosporum that has been cultured for 7 days, mix well and then culture for 7 days or 4 days. Then AO Sr The mycelium is obtained after filtering the fermented liquid with gauze.

[0039] 2. Detection of AO by fluorescence in situ hybridization Sr Stenotrophomonas rhizophila on hyphae ...

Embodiment 2

[0048] The present invention isolates Stenotrophomonas rhizophilus and its cyclic dipeptide metabolites from soil to induce Arthropodium oligosporum to form predatory organs:

[0049] 1. Preparation of AO Sr : Arthropodium oligospora was inoculated in liquid CMB, and cultured at 28° C. for 7 days. The formula of the CMB medium was: corn flour: 20 grams, 1000 milliliters of water, and the pH was natural. Stenotrophomonas rootophilus was cultured at 28° C. for 48 hours with a liquid medium, the formula of which was: peptone 5 g, beef extract 3 g, NaCl 5 g, distilled water 1000 ml, pH 7.2. Take 2.0ml of the fermentation broth of Stenotrophomonas rhizophilus and put it into Arthrobuspora oligosporum that has been cultured for 7 days, mix well and then culture for 3 days. Then AO Sr The mycelium is obtained after filtering the fermented liquid with gauze.

[0050] 2. Detection of AO by fluorescence in situ technique Sr Stenotrophomonas rhizophila on the surface of mycelium: det...

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Abstract

The invention relates to application of stenotrophomonas rhizophila and cyclic dipeptide metabolite thereof and belongs to the technical field of microbial pesticide. The stenotrophomonas rhizophila WQS37 strain is collected in the common microbial strain preservation administration center in Chinese microbial strain preservation administration committee on 5 November in 2012, and the collection number is CGMCC No.6777. The stenotrophomonas rhizophila is cultured and separated to obtain the cyclic dipeptide metabolite; and moreover, the stenotrophomonas rhizophila and cyclic dipeptide metabolite thereof and arthrobotrys oligospora are mixed, and the arthrobotrys oligospora is induced to rapidly form lots of capturing devices. The stenotrophomonas rhizophila and cyclic dipeptide metabolite can rapidly induce the arthrobotrys oligospora to form lots of capturing devices in soil, nematode can be effectively captured, and a novel thought is provided for developing a fungal microbial agent for capturing the nematode.

Description

Technical field: [0001] The invention relates to the application of Stenotrophomonas rootophilus and its cyclic dipeptide metabolites, and belongs to the technical field of microbial pesticides. Background technique: [0002] Plant parasitic nematodes are one of the important diseases of plants, and the economic losses of crops caused by plant parasitic nematode diseases reach 78 billion US dollars every year worldwide (McCarter, 2008). In my country, plant parasitic nematodes harm almost all crops such as tobacco, flowers, vegetables, cotton, soybeans, peanuts, and Chinese medicinal materials, and become one of the important limiting factors in agricultural production. At present, the control of nematodes mainly relies on chemical nematicides, but the long-term use of chemical pesticides not only causes environmental pollution, residues, and damage to ecological balance, but also aggravates the resistance of pests. The use of natural enemies of nematodes for biological con...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645C12R1/01
Inventor 李蕾罗俊张克勤马明川
Owner YUNNAN UNIV
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