Bio-enzyme hydrogel taking porous calcium carbonate as carrier and preparation method of bio-enzyme hydrogel

A technology of porous calcium carbonate and hydrogel, which is applied in medical preparations with non-active ingredients, medical preparations containing active ingredients, liquid delivery, etc. It can solve the problems of fast biological enzyme release rate and inability to meet clinical applications. , to achieve good air permeability, increase drug loading, and high water content

Active Publication Date: 2014-05-07
SHANGHAI HI TECH BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the release rate of biological enzymes from the hydrogel is too fast, and the cumulative release amount in 3 hours is 60%, which cannot meet the needs of clinical applications.

Method used

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  • Bio-enzyme hydrogel taking porous calcium carbonate as carrier and preparation method of bio-enzyme hydrogel

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] formula:

[0029] The content in weight percentage is: 0.01% of lysozyme; 15% of porous calcium carbonate; 1% of chitosan; 2% of gelatin; 15% of glycerin; 20% of ethanol;

[0030] (1) Pass carbon dioxide gas into the calcium hydroxide slurry with a weight content of 15% for carbonization. During the carbonization process, add ammonium persulfate solution and hydrazine hydrate solution independently at the same time. When the carbonization reaches the pH of the slurry When it is 10, stop adding ammonium persulfate solution and hydrazine hydrate solution in the slurry, continue carbon dioxide gas to carry out carbonization, until pH is 7, finish carbonization;

[0031] The mass percentage concentration of described ammonium persulfate solution is 20%;

[0032] The mass percentage concentration of described hydrazine hydrate solution is 30%;

[0033] The add-on of described ammonium persulfate is 3% of calcium hydroxide dry weight;

[0034] The add-on of described hydra...

Embodiment 2

[0042] formula:

[0043] The weight percent content is: 3% lysostaphin; 20% porous calcium carbonate; 10% chitosan; 2% gelatin; 15% glycerin; 20% ethanol;

[0044] Adopt the porous calcium carbonate of embodiment 1.

[0045] The method for preparing the biological enzyme hydrogel with porous calcium carbonate as the carrier is the same as in Example 1.

[0046] Detection method is with embodiment 1, and the results are shown in figure 1 .

Embodiment 3

[0048] formula:

[0049] The weight percentage content is: lysozyme 0.5%; lysostaphin 0.1%, porous calcium carbonate 17%; chitosan 5%; gelatin 2%; glycerin 12%; ethanol 17%; acetic acid 1%, water balance

[0050] Others are the same as embodiment 1, and the test results are shown in figure 1 .

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Abstract

The invention provides a bio-enzyme hydrogel taking porous calcium carbonate as a carrier and a preparation method of the bio-enzyme hydrogel. The bio-enzyme hydrogel comprises the following components in percentage by weight: 0.001-5 percent of bio-enzymes, 10-30 percent of porous calcium carbonate, 1-20 percent of chitosan, 0.1-2 percent of gelatin, 1-20 percent of glycerol, 10-30 percent of ethanol, 1-5 percent of acetic acid and the balance of water, wherein the bio-enzymes are more than one of lysozyme and lysostaphin. According to the bio-enzyme hydrogel taking porous calcium carbonate as the carrier, the porous calcium carbonate is added into hydrogel, so that not only is the chemical loading amount improved, but also the release rate of bio-enzymes can be adjusted. The bio-enzyme hydrogel taking porous calcium carbonate as the carrier has the characteristics of good antibacterial property, high water content, good water-retaining property, strong toughness, good air permeability and the like, has the capability of meeting the requirements of treating various wounds by a wet process and can be used for treating skin injuries, such as scalding, bedsore and surgical infections.

Description

technical field [0001] The invention relates to a hydrogel agent loaded with biological enzymes and a preparation method thereof. Background technique [0002] Biological enzymes, namely lysozyme and lysostaphin, are a new type of biological bactericidal enzymes, which have a specific killing effect on drug-resistant Staphylococcus aureus. In our previous patent, application number CN200810043907.0, a bio-enzyme-loaded hydrogel dressing has been prepared. The dressing can effectively kill the methoxy-resistant Staphylococcus aureus infection on the skin wound surface, and its sterilization rate reaches 99.9%. However, the release rate of biological enzymes from the hydrogel is too fast, and the cumulative release amount in 3 hours is 60%, which cannot meet the needs of clinical application. Contents of the invention [0003] The object of the present invention is to provide a kind of biological enzyme hydrogel agent with porous calcium carbonate as carrier, to overcome t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/47A61K9/06A61K47/04A61P31/04A61P17/02
Inventor 赵晓蔚解虹张赪陆婉英黄青山
Owner SHANGHAI HI TECH BIOENG
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