Synchronous saccharification and ethanol fermentation method with lignocelluloses treated by steam explosion method as raw material

Abstract

The invention provides a synchronous saccharification and ethanol fermentation method with lignocelluloses treated by a steam explosion method as raw material. The method comprises the following steps of taking the lignocelluloses treated by the steam explosion method as raw material and directly as a substrate without detoxification, adding mixed enzyme comprising cellulase and beta-glucosaccharase, simultaneously inoculating (Saccharomyces cerevisiae) Y5 with CGMCC (China General Microbiological Culture Collection Center) NO. 2660, adding a nitrogen source, and carrying out ethanol fermentation production. According to the method, the step of detoxification by washing the lignocelluloses (such as maize straw) pre-treated by steam explosion is omitted, and the final fermented product of ethanol of high concentration is obtained. For example, the concentration for steam explosion of the maize straw is 30%, synchronous saccharification and fermentation time is 96 hours, and the ethanol concentration of a reactor of 100ml, a reactor of 3000ml and a fermentation tank of 5L respectively reaches 50g / L, 47.8g / L and 47.5g / L. The production technology is greatly simplified, the equipment investment is lowered, water consumption is reduced, and the production cost is lowered.

Description

technical field [0001] The invention relates to the field of synchronous saccharification and ethanol fermentation, in particular to a synchronous saccharification and ethanol fermentation method using lignocellulose treated by a steam explosion method as a raw material. Background technique [0002] Lignocellulose is a cheap and widespread renewable resource. Using lignocellulose to produce fuel ethanol is an important direction of future energy development, which can alleviate the energy crisis and protect the environment at the same time. Steam explosion method is one of the lignocellulose pretreatment methods developed in recent years, but the bottleneck of producing fuel ethanol with steam explosion pretreated materials is the toxic substances such as acetic acid, furfural and 5-HMF produced in the steam explosion process Inhibition of yeast growth and fermentation. Therefore, the material after steam explosion treatment must be detoxified by water washing, physical, c...

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Problems solved by technology

However, biological detoxification also increases equipment investment and cost. At the same time, the SSF system uses a 1.3L system fermenter, but the actual effective volume is only 500ml, and the 20% (w / v) substrate concentration is not high, resulting in the fermentation broth The ethanol content is low, and the industrial production needs the ethanol base concentration in the fermentation broth to be more than 5%, so that the distillation of ethanol has economic benefits

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