Array type multiplex microfluidic isothermal amplification chip for HPV (human papillomavirus) detection

Abstract

The invention discloses an array type multiplex microfluidic isothermal amplification chip for HPV (human papillomavirus) detection, and the chip is made from polydimethylsiloxane and is prepared in a pouring method by utilizing a micro-processing technology. The basic structure of the chip comprises three parts, namely amplification pools, connecting pipelines and capillary channels. A series of the amplification pools which are orderly arranged in a space array are coated with nucleic acid probes through a freeze-drying technology, and the simultaneous detection of multiplex nucleic acid targets in a sample can be achieved by utilizing space differentiation of amplification signals; the capillary channels can achieve the pouring of a reaction solution and the sample through capillary pulling force, and a low-mass transfer coefficient is simultaneously utilized for effectively preventing the mutual interference of the nucleic acid probes, amplification products and the signals in different amplification pools; and the amplification pools and the capillary channels are communicated through the connecting pipelines for enabling fluid to smoothly and uniformly flow into the amplification pools. The chip is simple to manufacture and convenient to operate, and provides an effective scheme for simultaneous clinical detection of a variety of pathogens through an LAMP (isothermal amplification) method.

Description

technical field [0001] The invention relates to a multiple nucleic acid constant temperature amplification technology, in particular to an array type multiple microfluid constant temperature amplification chip. Background technique [0002] Human papillomavirus HPV is a genus of papillomavirus A belonging to the Papovaviridae family and is a spherical DNA virus. Divided into high-risk and low-risk types, low-risk HPV such as HPV-6, 11, 13, 32, 34, 40, 42, 43, 44, 53, 54, etc. are closely related to infection of genitals, anus, oropharynx, and esophageal mucosa , especially HPV6, 11, the most widespread incidence. Persistent infection of high-risk HPV is the main cause of cervical cancer, and HPV 16 and 18 are the two high-risk HPV most closely related to cervical cancer and the highest risk of disease. It accounts for at least 40% of cervical cancers. Timely and rapid distinction of different types of important HPV plays a vital role in the prevention and treatment of dis...

AI Technical Summary

Problems solved by technology

However, the "isothermal mode" of the LAMP reaction and its complex nucleic acid probes make it difficult to realize the simultaneous detection of multiple targets in a sample in the same system, that is, multiple detection