Method for enhancing beta-mannase yield by two-stage temperature control strategy
A mannanase and stage control technology is applied in the application field of the staged control strategy of environmental factors in the optimization of the fermentation process, and achieves the effects of increasing yield and simple control process.
Inactive Publication Date: 2013-05-22
JIANGNAN UNIV
View PDF3 Cites 2 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Using the temperature two-stage control method to increase the production of β-mannanase has not been reported in the literature.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0014] Embodiment: The bacterial strain that the present invention adopts is recombinant Bacillus subtilis BPM1003, and the control temperature is 37 ℃, stirring speed 400r / min, ventilation volume 1vvm in the growth stage of the thalli, and the thalline growth of 16 hours of fermentation culture basically reaches the stable period when the temperature The temperature was lowered to 25°C, the stirring speed was 400r / min, the ventilation rate was 1vvm, and the fermentation was completed for 65h, and the production of β-mannanase was 2207U / mL.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to a method for fermenting and producing beta-mannase by a two-stage temperature control strategy, belonging to the technical field of application of environmental factor staged control strategy in fermentation process optimization. The method comprises the following steps: by using recombinant Bacillus subtilis BPM1003 as an initial strain, fermenting and culturing at the controlled temperature of 37 DEG C for 16 hours in the thallus growth stage until the thallus growth basically reaches to the stabilization period, cooling the fermentation temperature to 25 DEG C, and fermenting for 40 hours. The fermentation by such strategy finally achieves the goal of enhancing the beta-mannase yield. The technique provided by the invention is simple and easy to implement, and can effectively enhance the beta-mannase yield. Meanwhile, the invention has certain instruction meanings for fermentation and production of other enzymes.
Description
technical field [0001] A method for fermenting and producing β-mannanase with a temperature two-stage control strategy relates to the technical field of application of environmental factor (temperature) stage control strategy in fermentation process optimization. Background technique [0002] β-mannanase (EC 3.2.1.78) is a hemicellulase that can hydrolyze β-mannosidic bonds and degrade some plant polysaccharides such as mannan, glucomannan, and galactomannan into Mannan oligosaccharides, together with cellulase and xylanase, constitute the most important enzyme system in the biotransformation of cellulosic substances. With the development of industrialization, human beings have an increasing demand for energy. Plant cell walls are composed of cellulose and hemicellulose, which are the most abundant renewable energy substances in the world. Effective use of them is the key to solving the energy crisis. way. The development and utilization of oligosaccharides formed by conne...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): C12N9/42C12R1/125
Inventor 饶志明徐美娟司冠儒满在伟杨套伟张显刘项羽
Owner JIANGNAN UNIV