Glucose detection test paper

A technology of glucose and glucose oxidase, which is applied in the field of detection test paper, to achieve the effect of reducing detection cost, reducing time, and improving stability

Inactive Publication Date: 2013-05-22
INST OF PROCESS ENG CHINESE ACAD OF SCI
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Embodiment 1

[0042] Embodiment 1 is used for the solution form reagent and detection method of glucose detection

[0043] This embodiment describes a determination reagent and detection method for total glucose detection, the content of each component in the reagent is as follows:

[0044] Glucose oxidase: 0.01g / L

[0045] Peroxidase: 0.01g / L

[0046] o-Dianisidine: 0.1mg / mL

[0047] Phosphate buffer at pH 7.0: 50mM

[0048] The detection method is to use a Unico 2800Uv-Vis spectrophotometer for detection, and the operation is as follows:

[0049]

[0050] Δ A440 (min -1 mg)—The working curve of glucose concentration is as follows: Δ A440 (min -1 )=0.0205*glucose concentration / μmol / L+0.0052; R 2 =0.996, detection linear range: 0-20mmol / L.

[0051] Detection of glucose concentration in the sample: Add 100 μL of glucose oxidase, 100 μL of peroxidase, 600 μL of o-dianisidine to the detection tube, then add 200 μL of the serum sample containing glucose to be detected, mix quickly a...

Embodiment 2

[0052] Preparation and detection method of embodiment 2 glucose detection test paper

[0053] Preparation of test paper for glucose detection: Dissolve 1 mg of glucose oxidase and 1 mg of peroxidase in 200 μL of water, then fully mix with 800 μL of glycerol, add the mixed solution to a 5 ml sampler, and use it as a test paper for coaxial electrospinning. Internal phase electrospinning solution: dissolving o-dianisidine in N,N-dimethylacetamide to prepare a 10mg / ml solution, and then fully dissolving a certain mass of polyurethane to prepare a 25% by weight solution The solution, as the external phase electrospinning solution, was added to another 5ml sampler; the internal and external phase electrospinning solutions were respectively connected to the liquid inlet of the coaxial electrospinning spinneret through pipelines, and the liquid outlet of the spinneret was Connect the port to the positive pole of the high-voltage power supply, and use aluminum foil or stainless steel m...

Embodiment 3

[0058] Preparation and detection method of embodiment 3 glucose detection test paper

[0059] Preparation of test paper for glucose detection: Dissolve 0.1mg of glucose oxidase and 0.5mg of peroxidase in 100μL of water, then fully mix with 900μL of glycerol, and add the mixture to a 5ml sampler as a coaxial electrode. Spinning internal phase electrospinning liquid; dissolving o-dianisidine in N,N-dimethylacetamide to prepare a 5 mg / ml solution, and then fully dissolving a certain mass of polyurethane to make a weight percentage of 20 % solution, as the external phase electrospinning solution, is added in another 5ml sampler; the internal and external phase electrospinning solutions are respectively connected to the liquid inlet of the coaxial electrospinning spinneret through pipelines, and the spinneret The liquid outlet is connected to the positive pole of the high-voltage power supply, and is connected to the negative pole of the high-voltage power supply with aluminum foil o...

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Abstract

The invention provides test paper and a detection method for glucose determination. According to the test paper, glucose oxidase and peroxidase are embedded in a cavity of a polymer, namely hollow nanofibers, in an in-situ manner by using a coaxial co-spinning electrospinning technique, and a color developing agent, namely o-dianisidine, is uniformly distributed on shell layer walls of the hollow nanofibers, and thus the glucose detection test paper with a macroscopic non-woven membrane morphology is obtained. When glucose liquid is dripped on the test paper, glucose is oxidized by the glucose oxidase, hydrogen peroxide is produced, and the colorless o-dianisidine is oxidized to a colored product under the catalysis of the peroxidase, so that the test paper can be subjected to color development, and the quantitative detection on the glucose is carried out through determining the color change of the test paper. A glucose detection reagent based on the hollow nanofibers has the outstanding advantages of simplicity in preparation, small enzyme consumption amount, simplicity and convenient in detection operation, and high storage stability and has an application prospect in the quick and accurate determination of the glucose.

Description

technical field [0001] The invention relates to a detection test paper for quantitative detection of glucose content, including a detection test paper for glucose detection and a detection method using the test paper, in particular to a glucose detection system based on an immobilized multi-enzyme system based on hollow nanofibers. Test strips. Background technique [0002] Diabetes is a worldwide frequently-occurring and common disease. With the improvement of people's living standards and the increase of the elderly population, its incidence is on the rise. The incidence rate in my country has reached 2.5-3%, which is very close to 2-5% in developed countries in Europe and America. There are more than 200 million patients in the world. It has become a global health care problem and seriously threatens human health. It has become the third most dangerous disease after cardiovascular disease and cancer. Therefore, the diagnosis and treatment of diabetes is not only a major...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/47
Inventor 张松平姬晓元苏志国王占丽王平
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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