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Rectum-massage electrical stimulating semen collecting method capable of improving semen collecting efficiency of pandas

A giant panda, electrical stimulation technology, applied in medical science, veterinary instruments, animal delivery, etc., can solve the problems of inconsistent actual stimulation current, animal vomiting, rectal mucosal damage, etc., to improve the success rate of semen collection and the amount of semen collected. The effect of improving and improving the success rate of semen collection

Inactive Publication Date: 2013-06-05
CHENGDU RES BASE OF GIANT PANDA BREEDING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Conventional giant panda electrical stimulation semen collection method uses voltage as the basis for electrical stimulation intensity, because the fecal residue in the giant panda rectum will directly affect the contact degree between the rectal probe and the rectal wall, thereby affecting the resistance value, resulting in the same stimulation voltage. The actual stimulation current is inconsistent, resulting in the same electrical stimulation program, the actual stimulation intensity is different, resulting in instability of semen collection efficiency
[0013] Conventional giant panda electric stimulation semen collection method simply uses electric stimulation to collect semen. The response of giant pandas to electric stimulation semen collection has individual differences. For individuals with poor response to electric stimulation, it is usually used to increase the number of electric stimulation times and increase the intensity of electric stimulation to obtain semen. Too much and too strong electrical stimulation may cause damage to the rectal mucosa
Electrical stimulation of semen collection is a painful operation, and the pain response caused by electrical stimulation may cause the animal to vomit, which increases the risk of anesthesia of animals during electrical stimulation of semen collection
Even if the intensity and frequency of electrical stimulation are increased for some individuals, the effect of semen collection is still not good, and even no semen is obtained

Method used

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  • Rectum-massage electrical stimulating semen collecting method capable of improving semen collecting efficiency of pandas

Examples

Experimental program
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Effect test

Embodiment 1

[0063] The present invention uses ketamine hydrochloride to anesthetize male giant pandas who have fasted for 12 hours, the anesthesia dose is 5-10 mg / kg, and isoflurane intubation is used to maintain anesthesia during the anesthesia process.

[0064] After securing the panda, enema with warm soapy water to remove residual feces, and clean the foreskin with normal saline.

[0065] Then proceed to the first series of stimulation procedures: first perform the rectal massage stimulation procedure, that is, put the index finger, middle finger and ring finger lubricated with paraffin oil together, probe into the rectum of a male giant panda 3 to 5 cm, explore the abdomen of the vas deferens, and use your fingers to place the finger on the vas deferens Massage the abdominal area back and forth 100-200 times; after the rectal massage stimulation procedure is over, slowly insert the rectal probe of the sperm collection device into the rectum of the male giant panda; then press 10mA, 10...

Embodiment 2

[0068] On the basis of Example 1, after the second series of stimulation procedures, the third series of stimulation procedures are carried out: first slowly take out the rectal probe from the rectum of the male giant panda; then carry out the rectal massage stimulation procedure; Slowly insert the probe into the rectum of the male giant panda; finally operate the sperm collection device in the order of 50mA, 10 times, 70mA, 10 times, 90mA, 10-20 times, 100mA, 10-20 times, and carry out the third current stimulation program , in the third current stimulation program, each current stimulation time is 2 seconds, and the time interval between two current stimulations is 3 seconds.

Embodiment 3

[0069] Embodiment 3: On the basis of Embodiment 2, after the end of the third series of stimulation procedures, the rectal probe was removed from the rectum of the male giant panda, and then the intestinal massage stimulation procedure was performed.

[0070] Embodiment 1 of the present invention is directed at male giant panda individuals who respond well to electrical stimulation, and Embodiment 2 and Embodiment 3 are directed at male giant panda individuals who do not respond well to electrical stimulation. If necessary, on the basis of Embodiment 3 , an additional current stimulation program can be performed.

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Abstract

The invention discloses a rectum-massage electrical stimulating semen collecting method capable of improving semen collecting efficiency of pandas. The rectum-massage electrical stimulating semen collecting method includes performing an electrical stimulating process adopting current as an index to an anesthetized male adult panda, and assisting with a rectum massage simulating process. Via the electrical stimulating process, stimulus intensity to the male panda is obviously reduced, and damage to the male panda is lowered; via the rectum massage simulating process, while quality of collected semen is guaranteed, 75% of the male pandas having bad reaction to the electrical stimulating process can be helped in collecting effect, so that amount of collected semen is obviously increased from 0.36+ / -0.66ml to 0.95+ / -0.99ml, and collecting success rate is obviously increased from 25% (3 / 12) to 83% (10 / 12).

Description

technical field [0001] The invention relates to a semen collection method for artificial insemination of captive animals, in particular to a rectal massage electric stimulation semen collection method for improving the efficiency of giant panda semen collection. Background technique [0002] The commonly used methods of semen collection in animals mainly include massage, false vagina and electrical stimulation. Massage and false vagina are widely used in cattle, sheep, pigs, chickens and other domestic animals; however, it is very difficult to collect semen with massage and false vagina for most wild animals that are wild, violent and timid . [0003] In 1934, Serebrovskii and Sokolovskaja successfully applied the electrostimulation semen collection method to the semen collection of waterfowl, and then, the electrostimulation semen collection method was widely used in other wild and commercial animals, such as deer, civet, bear, fox, wolf, Artificial semen collection of fo...

Claims

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Application Information

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IPC IPC(8): A61D19/02
Inventor 侯蓉张志和刘玉良兰景超王海瑞蔡志刚
Owner CHENGDU RES BASE OF GIANT PANDA BREEDING
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