Pancreatic cancer cell line with lymphatic channel high migration activity
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A technology for pancreatic cancer cell and lymph node metastasis, applied in the field of microbial animal cell lines, to achieve high transfer efficiency, important clinical significance and application prospects, and centralized transfer efficiency
Inactive Publication Date: 2013-06-05
FUDAN UNIV SHANGHAI CANCER CENT
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[0004] At present, there are no reports on pancreatic cancer cell lines with high lymphatic metastasis characteristics at home and abroad
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Embodiment 1
[0041] Example 1 Establishment of Human Pancreatic Cancer Cell Lines with High Lymphatic Metastatic Potential
[0042] 1. Cells and animals:
[0043] (1) Human pancreatic cancer cell line: BxPC-3, ATCC number: CRL1687. Purchased from the Cell Resource Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.
[0044] (2) Experimental animals: nude mice, male, 5-7 weeks old, 18-20 g. Raise them in an environment without special pathogens at a constant temperature of 25-27°C, place them on a laminar flow ultra-clean rack, and feed 4-6 nude mice in each breeding box.
[0045]2. Method: This experiment adopts the method of continuous inoculation and continuous screening in nude mice
[0046] (1) Subcutaneous inoculation on the paw pads of nude mice: Take 0.1ml of BxPC-3 cells (1~2×10 7 cell / ml) was inoculated at the subcutaneous position inside the left paw pad of the experimental nude mice, 6-8 per round; the inoculated nude mice were raised in an envi...
Embodiment 2
[0051] Example 2 BxPC-3-LN cell line lymphatic metastasis nude mouse model
[0052] 1. Cells and animals:
[0053] (1) Human pancreatic cancer cell line BxPC-3-LN with high lymphatic metastasis potential.
[0054] (2) Experimental animals: nude mice, male, 5-7 weeks old, 18-20 g. Raise them in an environment without special pathogens at a constant temperature of 25-27°C, place them on a laminar flow ultra-clean rack, and feed 4-6 nude mice in each breeding box.
[0055] 2. Method: Nude mouse model for lymphatic metastasis experiment
[0056] (1) Subcutaneous inoculation of paw pads of nude mice: take 0.1ml of BxPC-3-LN cells (1×10 7 cell / ml) were inoculated at the subcutaneous position inside the left paw pad of the above-mentioned nude mice, 8 in each group, and the inoculated nude mice were reared in an environment free of special pathogens. After 8 to 10 weeks, they were treated routinely, and the popliteal fossa on the same side was obtained under sterile conditions. ,...
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Abstract
The invention belongs to the field of microorganism animal cell lines, and particularly relates to a pancreatic cancer cell line with lymphatic channel high migration activity. According to the pancreatic cancer cell line with the lymphatic channel high migration activity, a human pancreatic cancer BxPC-3 cell line is adopted as source cells, by means of a nude mouse palmula subcutaneous vaccinization method, lymphatic metastasis oncocyte sunlines with different migration potentialities are separated from matrilineal BxPC-3 cells, a pancreatic cancer cell line BxPC-3-LN with the lymphatic channel high migration activity is screened out through experiments inside and outside human bodies, a storage number is CGMCC NO.5421, and the storage date is October 28th, 2011. The pancreatic cancer cell line with the lymphatic channel high migration activity is the same as the matrilineal BxPC-3 cells in the genetic background, is characterized by being strong in invasiveness, high in migration efficiency and concentrated in migration, provides a suitable experimental platform for research on pancreatic cancer migration mechanism and the screening of antineoplastic drugs, and has very important significance and wide application prospects.
Description
technical field [0001] The invention belongs to the field of microbial animal cell lines, and in particular relates to a pancreatic cancer cell line with high lymphatic metastasis activity. Background technique [0002] Pancreatic cancer is a malignant tumor that seriously endangers human health. According to reports, the incidence of pancreatic cancer has been on the rise worldwide in recent years. Pancreatic cancer has special biological behavior, early tumor metastasis, low surgical resection rate, and poor clinical treatment effect. Clinical studies have shown that lymphatic metastasis is the main way of pancreatic cancer metastasis, and it is also a decisive factor affecting its prognosis. Its characteristics are as follows: ① Metastasis occurs early, even for small pancreatic cancers less than 2cm, 50% of them have lymphatic metastasis; ②The incidence of metastasis is high, and the incidence of lymph node metastasis in pancreatic head cancer is as high as 71.2%; ③The ...
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