Method for seed germination of jerusalem artichoke
A seed and Jerusalem artichoke technology, applied in the field of restoration of heat suppression of seed germination, can solve problems such as seed germination, achieve the effects of preventing germplasm degradation, high reproduction coefficient, and reducing planting costs
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Embodiment 1
[0015] 1. Take 50 Jerusalem artichoke seeds at random, and treat the Jerusalem artichoke seeds with inhibited germination and fever for 2 days under the temperature condition of 7℃ and alternate light conditions to restore the germination of the Jerusalem artichoke seeds with inhibited germination and fever. The alternate lighting conditions are: 14 hours of light per day and 10 hours of darkness ; Place the treated Jerusalem artichoke seeds on a rooting medium with 1 / 2MS as a basic medium, containing 30 mg / L of sucrose, 7 mg / L of agar, 0.1 mg / L of NAA, and a pH of 5.6; the intensity of light is specifically 1500 Lux.
[0016] 2. When the test tube shoots grow to 2cm, they can be directly cut and transferred to the rooting medium for rooting culture, without strong seedling culture. When the roots grow to 3cm, they can be cultivated and then transplanted to the field.
[0017] 3. The specific steps of refining seedlings are as follows: Loosen the cap of the test tube, 2 days; Add ...
Embodiment 2
[0019] 1. Take 50 Helianthus tuberosus seeds at random, and treat the Helianthus tuberosus seeds with inhibited germination and fever for 5 days under the temperature condition of 21℃ and alternate light conditions to restore the germination of Helianthus tuberosus seeds with inhibited germination and fever. The alternate lighting conditions are: daily light for 12 hours and dark for 12 hours ; Place the treated Jerusalem artichoke seeds on a rooting medium with 1 / 2MS as a basic medium, containing 30 mg / L of sucrose, 7 mg / L of agar, 0.1 mg / L of NAA, and a pH of 5.7; the intensity of light is specifically 1800 Lux.
[0020] 2. When the test tube seedling shoot grows to 3cm, it can be directly cut and transferred to the rooting medium for rooting culture without strong seedling culture. When the root grows to 4cm, it can be cultivated by tempering seedlings, and finally transplanted to field cultivation.
[0021] 3. The specific steps of refining seedlings are as follows: Loosen the ...
Embodiment 3
[0023] 1. Take 50 Helianthus tuberosus seeds at random, and treat the Helianthus tuberosus seeds with inhibited germination and fever for 3 days under the temperature condition of 15℃ and alternate light conditions to restore the germination of Helianthus tuberosus seeds with inhibited germination and fever. The alternate lighting conditions are: 13 hours of light per day and 11 hours of darkness ; Place the treated Jerusalem artichoke seeds on a rooting medium with 1 / 2MS as a basic medium, containing 30 mg / L of sucrose, 7 mg / L of agar, 0.1 mg / L of NAA, and a pH of 5.6; the intensity of light is specifically 1700 Lux.
[0024] 2. When the test tube shoots grow to 2cm, they can be directly cut off and transferred to the rooting medium for rooting culture, without strong seedling culture. When the roots grow to 4cm, they can be refined and transplanted to the field.
[0025] 3. The specific steps of refining treatment are as follows: Loosen the cap of the test tube, 2 days; Add ster...
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