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Enzymic-method based preparation method of soybean peptide

An enzymatic preparation, soybean peptide technology, applied in the preparation methods of peptides, chemical instruments and methods, peptides and other directions, can solve problems such as insufficient enzymatic properties, and achieve the effects of easy absorption by the human body, small molecular weight, and improved hydrolysis efficiency

Active Publication Date: 2014-11-19
珠海博康药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Earthworm serine proteases are a group of proteolytic enzymes, but the enzymatic properties of them are not well studied

Method used

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Examples

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Embodiment Construction

[0023] For a better understanding of the present invention, the present invention will be further specifically described below through the following examples, but it should not be construed as a limitation of the present invention. For some non-essential improvements and adjustments made by those skilled in the art according to the above-mentioned content of the invention, It is also considered to fall within the protection scope of the present invention.

[0024] We adopt the method disclosed in the present invention to carry out the following operations:

[0025] (1) Weigh 500g of soybean protein isolate, collected from Fuji (Beijing) Technology Co., Ltd., food grade, add 2000ml of purified water according to the w / v material ratio of 1:40, and sterilize at 80°C for 30min (the temperature should not be too high, Prevent protein denaturation) cooling to 40 ℃;

[0026] (2) Add 0.5 g of earthworm serine protease according to 1‰ of soybean protein isolate weight (source: Zhuhai...

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Abstract

The invention provides an enzymic-method based preparation method of soybean peptide, wherein earthworm serine protease is creatively employed as zymogen for enzymolysis of soybean, thereby separating protein, wheat protein and the like; the hydrolysis efficiency is high; the ultrafiltration technology is employed in the preparation process so that the obtained enzymolysis product soybean peptide is separated more thoroughly; the yield is high; the conditions of the whole process are moderate; and the method is environment-friendly.

Description

technical field [0001] The invention relates to a preparation process of soybean peptide, in particular to an enzymatic preparation method of soybean peptide. Background technique [0002] After soybean protein is hydrolyzed by enzymes, a mixed hydrolyzate composed of various peptide molecules is obtained, which is called soybean peptide. Soybean peptide and soybean protein have exactly the same amino acid composition, generally composed of 2 to 10 amino acid residues, with a good balance of essential amino acids. Compared with soybean protein, soybean peptide has good processing characteristics and unique physiological activity, and can be used in the production of various foods and even cosmetics. [0003] The research on soybean protein hydrolyzate initially used acid-base chemical reagents to carry out the hydrolysis reaction at a certain temperature, but it was found in the research that there are various disadvantages in the preparation of soybean peptides by chemical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 陈东黄家升方启舟
Owner 珠海博康药业有限公司