Method for efficiently preparing low-molecular-weight fish skin collagen peptide through enzyme method

A fish skin collagen and low molecular weight technology is applied in the field of enzymatically efficient preparation of low molecular weight fish skin collagen peptides. Absorbability and other issues, to achieve high water solubility and digestion and absorption, good environmental and social benefits, and uniform distribution.

Inactive Publication Date: 2016-02-24
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on cold-adapted collagenase for preparing fish skin collagen peptides by enzymatic hydrolysis of fish skin, and there is no commercialized cold-adapted fish skin collagenase
[0004] At present, most of the commercialized collagen peptides on the market are prepared from a variety of commercial enzyme preparations to enzymolyze collagen materials. There are two common problems: (1) the molecular weight range is widely distributed and the molecular weight of the peptide segment is relatively high. From 1,000 to 10,000, it cannot meet the market demand for small peptide products with smaller molecular weight and uniform distribution, and studies have shown that short peptide chains of 2 to 5 amino acids can directly enter the blood through two types of peptide transport carriers, namely the average molecular weight Small peptides with low average molecular weight are absorbed faster, and short peptides with high average molecular weight are significantly affected by the gastric digestion process, that is, the higher the molecular weight, the water solubility and tissue absorbability of small peptides will be affected to varying degrees
(2) The product yield of enzymatically hydrolyzing fish skin to prepare collagen peptides is low. After the enzymolysis process ends, a large amount of unenzymolyzed fish skin substrates remain, resulting in a large amount of waste of fish skin raw materials and increasing production costs. Seriously reduced business efficiency

Method used

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  • Method for efficiently preparing low-molecular-weight fish skin collagen peptide through enzyme method
  • Method for efficiently preparing low-molecular-weight fish skin collagen peptide through enzyme method
  • Method for efficiently preparing low-molecular-weight fish skin collagen peptide through enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: A small-scale preparation method of fish skin collagen peptide, the steps are as follows:

[0042] (i) After activating the deep-sea bacteria Pseudoalteromonas sp.SM9913 on the plate for three consecutive times, pick a single colony to inoculate the liquid seed medium, shake culture at 15-20℃ for 24h, and then press 1% (v / v) inoculum Inoculate the fermentation medium, cultivate at 15°C for 72-84h, 4°C, 12000rpm centrifugation to prepare MCP-01 crude enzyme solution, and store at 4°C at low temperature. The components of the solid medium are as follows, all in parts by weight:

[0043] 0.8-1.0 parts of peptone, 0.5-0.75 parts of yeast powder, 1.0-1.5 parts of agar, 100 parts of artificial sea water, pH 7.5-8.0;

[0044] The components of the liquid seed medium are as follows, all by weight:

[0045] 0.8-1.0 parts of peptone, 0.5-0.75 parts of yeast powder, 100 parts of artificial sea water, pH 7.5-8.0;

[0046] The components of the fermentation medium are as follows,...

Embodiment 2

[0054] An enzymatic method for efficiently preparing low molecular weight fish skin collagen peptides includes the following steps:

[0055] (1) After breaking the pretreated fish skin, add MCP-01 enzyme solution in an amount of 1 U / g. The ratio of the broken fish skin to the volume of the reaction system in the reaction system is 0.5:1, unit g / mL, at 37°C, 150 rpm, and pH 7, after enzymatic hydrolysis for 2 hours, the reaction solution was prepared;

[0056] The pretreated fish skin is the fish skin after fat removal and ash removal. The fat removal steps are as follows:

[0057] After the fish skin is rinsed with water, it is soaked in a NaOH solution with a concentration of 0.08M for 14 hours, washed with water until it is neutral, and the fatty fish skin is obtained;

[0058] The steps to remove ash are as follows:

[0059] Immerse the fat-removed fish skin in hydrochloric acid with a concentration of 0.25M for 5 hours, wash with water until it is neutral, and obtain the ash-remov...

Embodiment 3

[0071] An enzymatic method for efficiently preparing low molecular weight fish skin collagen peptides includes the following steps:

[0072] (1) After breaking the pretreated fish skin, add MCP-01 enzyme solution in an amount of 30 U / g. The ratio of the broken fish skin to the volume of the reaction system in the reaction system is 1:1, unit g / mL, under the conditions of 40℃, 170rpm, pH 7.5, after enzymatic hydrolysis 0.5, the reaction solution is prepared;

[0073] The pretreated fish skin is the fish skin after the fat and ash have been removed. The fat removal steps are as follows:

[0074] After the fish skin is rinsed with water, it is soaked in NaOH solution with a concentration of 0.12M for 10 hours, and the water is washed until it is neutral to obtain a fatty fish skin;

[0075] The steps to remove ash are as follows:

[0076] Immerse the fat-removed fish skin in hydrochloric acid with a concentration of 0.35M for 3 hours, and wash the paper to be neutral to obtain ash-remove...

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Abstract

The invention relates to a method for efficiently preparing low-molecular-weight fish skin collagen peptide through an enzyme method. The method comprises the following steps: (1), after preprocessed fish skin is smashed, adding MCP-01 enzyme liquid and carrying out enzymolysis, so as to obtain reaction liquid; (2), carrying out enzyme inactivation on the reaction liquid obtained in the step (1), then carrying out solid-liquid separation and purifying, so as to obtain the low-molecular-weight fish skin collagen peptide. The low-molecular-weight fish skin collagen peptide prepared through the method provided by the invention has the advantages of low molecular weight, high peptide content, high product yield and the like, thereby having higher water solubility and tissue absorbability, and an excellent commercial application prospect.

Description

Technical field [0001] The invention relates to an enzymatic method for efficiently preparing low molecular weight fish skin collagen peptides, in particular to a method for efficiently preparing low molecular weight fish skin collagen peptides by using marine collagenase MCP-01, which belongs to the technical field of biotechnology. Background technique [0002] my country’s fishery industry has a long history. The output of aquatic products accounts for 1 / 3 of the world’s. It has 1 / 4 of the world’s fishing boats, 1 / 6 of the catch, and nearly 70% of the aquaculture output. The country has about 14 million fishery labors, 7.8 million hectares of farming area, 1 million fishing boats, and fishery output value of 675.2 billion yuan. In 2010, my country’s aquatic product output was 53.73 million tons, accounting for about 30% of global output. As a large aquaculture country, my country accounts for more than 70% of the world's aquaculture production. According to statistics from t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 张玉忠陈秀兰李静张熙颖宋晓妍解彬彬苏海楠秦启龙周百成
Owner SHANDONG UNIV
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