Method for establishing tree-shrew breast cancer model for slow virus by nipple injection

A lentivirus and breast cancer technology, applied in the field of making animal models of human diseases, can solve the problems of long time-consuming cycle, low induction rate, poor model consistency, etc., and achieve good model consistency, short latency time, and high occurrence efficiency. Effect

Active Publication Date: 2013-06-26
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional breast cancer induction methods such as the chemical carcinogen DMBA tak

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] In order to make the present invention easier to understand, specific implementation examples of the present invention will be further described below.

[0025] 1. Construction of viral vectors

[0026] The polyoma intermediate T antigen (PyMT), EGFP lentiviral expression vector and packaging vector plasmids used in the examples of the present invention were provided by the collaborator Li Yi, Baylor College of Medicine, USA. The virus vector used is a conventional lentivirus vector, and the virus particles packaged in it can infect and integrate its gene into the genome of tree shrew mammary gland epithelial cells.

[0027] 2. Virus packaging and titer determination

[0028] HEK293T cells were co-transfected with PyMT, EGFP gene expression vector FU-CGW and packaging vectors pRev, pMDL and pVSVG respectively, and the fresh DMEM medium was replaced 24 hours after transfection, and the culture supernatant was collected 48 hours after transfection, and removed by low-spe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for establishing a tree-shrew breast cancer model for slow virus by nipple injection. Slow virus is introduced to a tree-shrew breast duct by adopting a nipple injection technology, so that a cancer gene is over-expressed or an anti-cancer gene is knocked down after epithelial cells in a mediated duct are infected, and therefore, tree-shrew breast tumors are induced. According to the method for establishing a tree-shrew breast cancer model for slow virus by nipple injection disclosed by the invention, the breast tumors can be touched within two weeks after expressing the cancer gene PyMT; and moreover, the induction success rate is almost 100%, and the method is short in incubation period, strong in specificity, extremely high in induction rate, stable and convenient and simple to operate. The method disclosed by the invention can be used for quickly and efficiently establishing the tree-shrew breast cancer model.

Description

technical field [0001] The invention relates to the technical field of making animal models of human diseases, in particular to a method for establishing a tree shrew breast cancer model induced by lentivirus-mediated overexpression of oncogenes or knockdown of tumor suppressor genes. Background technique [0002] At present, breast cancer research is mainly based on breast cancer models established by rodents, but they cannot fully simulate human diseases because of their great differences from humans. Although non-human primates are the most ideal model animals, they are expensive. Tree shrews are second only to higher primates in terms of biological taxonomy, closer to humans than rodents, and are cheap. [0003] As early as 1966, Elliot et al first reported a case of spontaneous breast cancer in tree shrews in "Nature". Afterwards, there were no reports on tree shrew mammary gland tumor models at home and abroad. In the previous work of our research group, 5 cases of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/867C12N7/01A01K67/027
Inventor 陈策实夏厚军何保丽角建林
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap