Method for preparing coating carrying growth factor on surface of bone injury repair material

Abstract

The invention discloses a method for preparing a coating carrying out a growth factor on the surface of a bone injury repair material. According to the method, 5 to 100 unit films are formed on the surface of the material through a layer-by-layer self-assembly process; four layers of unit films comprise a polyelectrolyte layer (polycation layer with amino) with positive charges, a polysaccharide oxide layer (with negative charges), a growth factor embedding micro and nano particle layer with positive charges and a polysaccharide oxide layer (with negative charges) from inside to outside. Micro and nano particles with negative charges in the unit films are sandwiched between the two polysaccharide oxide layers with the negative charges through electrostatic adsorption, and the amino carried by the micro and nano particles can be covalently bound with the aldehyde group of polysaccharide oxides, so that the micro and nano particles are well immobilized in the unit films. The unit films can be covalently bound and crosslinked with each other through the polycation with the amino and the polysaccharide oxide with the aldehyde group, achieve an electrostatic adsorption effect due to opposite electric properties, and have strong binding force; and the coating is high in stability, slow in degradation, long and controllable in bone induction acting time and good in bone induction effect.

Description

technical field [0001] The invention relates to a method for preparing a growth factor-loaded coating on the surface of a bone damage repairing material. Background technique [0002] Due to their good mechanical properties and biocompatibility, metal medical material alloys are often used as bone repair materials, that is, bone tissue replacement materials, to be implanted in the human body. After implanted into the human body, the interaction between bone repair materials and the surrounding environment in the body first occurs on the surface, and metal materials are biologically inert materials, so it is very important to modify the surface of the implant. In recent years, the method of modifying the surface of bone repair materials has been widely used clinically, especially the method of immobilizing growth factors on the surface of the material to endow the material with osteoinductivity has been extensively studied. [0003] The surface of the material builds a polye...

AI Technical Summary

Problems solved by technology

Generally, the construction of polyelectrolyte multilayer films relies on the alternating deposition of positive and negative charges, and the molecular layers are assembled by electrostatic adsorption. This non-crosslinked self-assembled film will lead to the deposition of polyelectrolytes, which are originally weak in electricity, on the surface of the material. Insufficient strength, the membrane unit layer is easy to degrade and interdiffused in the biological environment, and the stability is not high. On the other hand, growth factors and other biologically active molecules are assembled between the membrane layers through their own electricity and the polyelectrolyte layer through electrostatic interaction Or directly chemically grafted with the self-assembled membrane, which also enables the rapid release of these biomolecules with the degradation of the polyelectrolyte membrane

Therefore, the osteoinductive action time of biomolecules is short and the effect is poor