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Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same

A compound microbial inoculant, Bacillus technology, applied in microorganism-based methods, biochemical equipment and methods, chemicals for biological control, etc., can solve the non-degradability of chemical pesticides, pollution enrichment, beneficial biological Threats and other issues, to achieve the effect of reducing the frequency of fertilizer use and the cost of use

Active Publication Date: 2015-03-04
河北省微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because of their non-selectivity, chemical pesticides can also pose a threat to beneficial organisms, and the non-degradability of chemical pesticides can easily accumulate in animals and plants, resulting in the enrichment of pollutants

Method used

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  • Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same
  • Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same
  • Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A jelly-like Paenibacillus mucilaginosus, the preservation number of which is CGMCC No.7240.

[0043] The method for producing composite microbial bacterial agent by using Paenibacillus jelly-like comprises the following process steps:

[0044] A. to expand training

[0045] The original strains are enlarged and cultivated to make production strains;

[0046] Paenibacillus mucilaginosus is selected as the original strain, and its preservation number is CGMCC No.7240;

[0047] B. Preparation of fermentation broth

[0048] B1, fermentation

[0049] According to the volume ratio of the production strain: fermentation medium = 10%, the production strain is inoculated into the sterilized fermentation medium for fermentation. The fermentation conditions are: temperature 25 ° C, pressure 0.03 MPa, ventilation control conditions are : 200M after fermentation starts 3 / hr, 320M after 4 hours of fermentation 3 / hr, 420M after 8 hours of fermentation 3 / hr, 520M after 12 ho...

Embodiment 2

[0068] The method for producing composite microbial bacterial agent by using Paenibacillus jelly-like comprises the following process steps:

[0069] A. to expand training

[0070] The original strains are expanded and cultivated to make production strains;

[0071] Paenibacillus mucilaginosus is selected as the original strain, and its preservation number is CGMCC No.7240;

[0072] B. Preparation of fermentation broth

[0073] B1, fermentation

[0074] According to the volume ratio of the production strain: fermentation medium = 15%, the production strain is inoculated into the sterilized fermentation medium for fermentation. The fermentation conditions are: temperature 32°C, pressure 0.7MPa, ventilation control conditions are : 250M after fermentation starts 3 / hr, 360M after 4 hours of fermentation 3 / hr, 70M after 8 hours of fermentation 3 / hr, 560M after 12 hours of fermentation 3 / hr, 630M after 20 hours of fermentation 3 / hr, start stirring after 8 hours of ferm...

Embodiment 3

[0093] The method for producing composite microbial bacterial agent by using Paenibacillus jelly-like comprises the following process steps:

[0094] A. to expand training

[0095] The original strains are enlarged and cultivated to make production strains;

[0096] Paenibacillus mucilaginosus is selected as the original strain, and its preservation number is CGMCC No.7240;

[0097] B. Preparation of fermentation broth

[0098] B1, fermentation

[0099] According to the volume ratio of the production strain: fermentation medium = 12%, the production strain is inoculated into the sterilized fermentation medium for fermentation. The fermentation conditions are: temperature 30 ° C, pressure 0.2 MPa, ventilation control conditions are : 230M after fermentation starts 3 / hr, 350M after 4 hours of fermentation 3 / hr, 450M after 8 hours of fermentation 3 / hr, 550M after 12 hours of fermentation 3 / hr, 620M after 20 hours of fermentation 3 / hr, start stirring after 6 hours of ...

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Abstract

The invention belongs to preparation of a microbial agent and especially relates to paenibacillus mucilaginosus and a method for producing a compound microorganism bacterium agent by utilizing the same. After the paenibacillus mucilaginosus CGMCC No. 7240 is selected and fermented, the fermentation liquid is combined with oligochitosan and corresponding microelements to form the compound microorganism bacterium agent. According to the invention, the problems of no mature product of environmental-friendly bio-agricultural preparation and large potential market gap in the prior art are solved; the produced pollution-free green biological preparation has double functions of pesticide and fertilizer; therefore, in use, the times of fertilizer application and the use cost can be reduced and the synergistic effect of mutual promotion and mutual improvement between the pesticide and the fertilizer is achieved; and as a result, obvious environmental value and social benefits can be generated.

Description

technical field [0001] The invention belongs to the preparation of microbial preparations, in particular to a jelly-like Paenibacillus and a method for producing composite microbial preparations using it. Background technique [0002] For a long time, the problem of environmental pollution caused by the extensive use of chemical pesticides has become increasingly serious. Because of their non-selectivity, chemical pesticides can also pose a threat to beneficial organisms, and the non-degradability of chemical pesticides can easily accumulate in animals and plants, resulting in the enrichment of pollutants. As the public's requirements for "green food" and "pollution-free food" are getting higher and higher, the market's demand for non-polluting and degradable biopesticides is gradually increasing. From the perspective of the development trend of pesticide research and production, new Green biological source agricultural preparations have broad prospects for development and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02C05F11/08A01P1/00C12R1/01
Inventor 李军秦艳梅赵从波王云鹏马清河章淑艳陈文杰古述江韩韬王鹏
Owner 河北省微生物研究所有限公司
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