Nucleic acid chromatography detection kit of Candida albicans, detection method and application thereof

A technology of candida nucleic acid and detection kit, which is applied in the fields of molecular biology and immunology, can solve the problems of cumbersome steps and long detection time, and achieve the effect of simple operation process, low detection cost and lower requirements

Inactive Publication Date: 2013-07-10
北京陆桥技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem solved by the present invention is to provide a nucleic acid chromatography detection ki...

Method used

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  • Nucleic acid chromatography detection kit of Candida albicans, detection method and application thereof
  • Nucleic acid chromatography detection kit of Candida albicans, detection method and application thereof
  • Nucleic acid chromatography detection kit of Candida albicans, detection method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0059] The detection specificity of embodiment 1 kit

[0060] Candida portuguese ATCC34449 (1), Candida kefir ATCC66028 (2), Candida krusei ATCC14243 (3), Candida tropicalis ATCC13803 (4), Candida utilis ATCC9950 (5), pseudosmooth Trichosanthes ATCC22019 (6), Candida glabrata ATCC15126 (7) are verified strains of Candida, and other common bacterial pathogenic bacteria Escherichia coli (8), Staphylococcus aureus (9), and Salmonella enteritidis (10), the above genomes were extracted As a template for PCR amplification, Candida albicans ATCC26790 was used as a positive control C + , see the test results figure 2 .

[0061] It can be seen from the results in the figure that only in the positive control (C + ) 26790 places of Candida albicans were positive, and the rest were negative, indicating that the detection kit of the present invention has good detection specificity and accurate detection results.

Embodiment 2

[0062] The sensitivity of embodiment 2 kits

[0063] The overnight cultivated Candida albicans ATCC26790 standard strain was diluted with sterile saline to select 10 2 CFU / mL, 10 3 CFU / mL, 10 4 CFU / mL, 10 5 CFU / mL, 10 6 Each of the three concentrations of CFU / mL gets 1 mL, detects according to the method of the present invention, judges the detection results, and does five parallels at the same time, and detects the lowest concentration of positive results as the detection sensitivity of the target bacterial strain, the results are shown in image 3 .

[0064] From the results, it can be seen that in 10 3 Positive at CFU / mL, 10 2 The detection line at the CFU / mL place does not develop color and the result is negative, indicating that the sensitivity of the kit of the present invention to detect Candida albicans can reach 10 3 CFU / mL.

Embodiment 3

[0065] Example 3 Sample Detection

[0066] Obtain 60 copies of clinical samples from medical testing institutions, use the kit of the present invention to detect, and use the microbial culture detection method (Komaga Candida chromogenic medium) to carry out comparative verification at the same time, the results are shown in Table 1, and the results of the microbial culture detection method There was no significant difference in comparison, the specificity reached 97.1%, and the coincidence rate was 98.3%.

[0067] Table 1 Sample detection test

[0068]

[0069] x 2 =0 P>0.05.

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Abstract

The invention discloses a nucleic acid chromatography detection kit of Candida albicans, a detection method and an application thereof, belonging to a field of molecular biology and immunology. According to the invention, polymerase chain reaction of molecular biology and immunochromatography are combined, thereby realizing rapid detection of the Candida albicans. The kit comprises PCR amplification and test strip detection, wherein primers are subjected to specific modifications, corresponding coating objects and tags are selected during preparation of the test strips, and a specific compound is formed during the reaction process, thereby realizing combination of the molecular biology technology and the immunochromatography technology, and realizing detection of specific amplification products of the Candida albicans. Compared with traditional biochemical method, the method greatly saves detection time, simplifies operation steps, and achieves purposes of rapid, high-efficiency and accurat detection and screening. The kit plays an important effect on clinic detection and screening process of the Candida albicans.

Description

technical field [0001] The invention belongs to the fields of molecular biology and immunology, and relates to a kit, method and application for rapid detection of Candida albicans. Background technique [0002] Candida albicans is the most common species of the genus Candida (Candida). It is also an important pathogen of candidiasis with the highest incidence rate among superficial / deep mycoses. Because the bacteria often invade patients with severe diseases and low immunity, as well as surgical patients who undergo organ transplantation and heart and gastrointestinal surgery that require intubation, urinary catheters, and catheters, it is a great threat to life. Discovery and diagnosis of Candida infection, rapid, simple, specific and accurate methods for clinical isolation and identification of Candida have been widely concerned at home and abroad. With the continuous expansion of immunocompromised or low population, opportunistic candidiasis continues to increase, so f...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04G01N33/569G01N33/558C12R1/725
Inventor 何艳玲王丽丽刘沛于重楠
Owner 北京陆桥技术股份有限公司
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