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A3 subunit protein polypeptide nutritional emulsifier and preparation method thereof

A protein polypeptide and emulsifier technology, which can be used in dairy products, milk replacers, applications, etc., and can solve the problem of high preparation cost

Active Publication Date: 2015-08-05
NANCHANG CAOSHANHU TECH IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The preparation of A3 subunit protein polypeptide in the prior art has the defect of high preparation cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] The method of preparing the A3 subunit protein polypeptide nutritional emulsifier using defatted soybean meal is as follows:

[0016] The first step: use the low-temperature defatted soybean meal solution in an ice bath at 0±2°C for 4 hours, remove the supernatant, and adjust the pH to 8;

[0017] Step 2: Fully stir and extract the alkali-soluble soybean 11S globulin, centrifuge, and separate the extract by circulating membrane to form a retentate. The molecular weight of the permeated molecules is 302,000-375,000 Daltons and 38,000-43,000 Daltons pause;

[0018] Step 3: Use magnetically immobilized pepsin to enzymatically hydrolyze the retentate, decolorize and remove bitterness with activated carbon, and perform secondary membrane separation and fractional purification to obtain small molecule peptides with a molecular weight of 1000-5000 Daltons;

[0019] Step 4: Roller-dry the fractionated and purified small molecule peptide liquid to obtain protein peptide powder ...

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Abstract

The invention relates to an A3 subunit protein polypeptide nutritional emulsifier, which is prepared by conducting enzymolysis on a defatted soybean meal solution by a magnetic immobilized pepsin and comprises no less than 90% of a crude protein, no less than 80% of peptide and has peptide molecular weight no more than 2000Da. The invention has the beneficial effect that: the magnetic immobilized pepsin is used to conduct enzymolysis on a soybean 11S globulin, so as to rapidly, conveniently and efficiently obtain an A3 subunit protein peptide powder.

Description

technical field [0001] The invention relates to the field of health food, in particular to a polypeptide composition obtained by using defatted soybean meal to prepare A3 subunit protein and enzymatically hydrolyzing it. Background technique [0002] Most of the protein ingested by humans is digested and absorbed in the form of low peptides after being acted on by enzymes in the digestive tract, and the proportion absorbed in the form of free amino acids is very small. It is found through experiments that peptides are digested faster and absorbed more than free amino acids, indicating that peptides have higher biological potency and nutritional value than free amino acids. Active peptides have a variety of human metabolism and physiological regulation functions, are easy to digest and absorb, and have the functions of promoting immunity, hormone regulation, antibacterial, antiviral, lowering blood pressure, and lowering blood fat. They are extremely safe to eat and are curre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23C11/10A23L11/65
Inventor 李国勇
Owner NANCHANG CAOSHANHU TECH IND
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