Method for determining biomass of hyphae of edible fungi in substrate by detecting DNA (Deoxyribonucleic Acid) concentration

Abstract

The invention relates to a method for determining the biomass of hyphae of edible fungi in a substrate by detecting the DNA (Deoxyribonucleic Acid) concentration, comprising the following steps of: (1) culturing pure hyphae of the edible fungi; (2) filtering, washing, freezing and fully drying the hyphae; (3) gradually weighing the hyphae, extracting DNA and then establishing an associated standard curve; (4) extracting the DNA from the relevant substrate and then determining the background level of the DNA in the substrate; (5) mixing the hyphae with the substrate, extracting the DNA, determining DNA amount, and comparing the result with the results obtained in the steps (3) and (4) to determine the influence of the substrate on DNA extraction; and (6) extracting the DNA from a sample tobe detected, determining the DNA amount, and then comparing the result with the standard curve, the substrate background and the substrate influence to determine the biomass of the hyphae of the edible fungi in the sample containing the substrate. The method has the advantages of simplicity, short consumed time, high efficiency and accuracy and can be used for stably monitoring the biomass of thehyphae of the edible fungi in a relevant culture mode or growing environment for a long term by adopting the determination of a detecting system.

Description

technical field [0001] The invention belongs to the field of methods for measuring the mycelial biomass of edible fungi, in particular to a method for measuring the mycelial biomass of edible fungi in a cultivation substrate by detecting DNA concentration. Background technique [0002] Biomass is a basic parameter of the growth status of all microorganisms, and the determination of biomass is the basis for determining the growth kinetics of the microbial culture process and for in-depth research on the regulation of microbial metabolism. Due to the mixed characteristics of most microorganisms and their growth substrates, it is difficult to directly measure their biomass, especially in artificially cultivated edible fungi where the hyphae penetrate deep into the culture substrate and cannot be separated and measured. [0003] There are four types of existing microbial biomass measurement methods: (1) separate and count directly from the culture medium, such as turbidity metho...

AI Technical Summary

Problems solved by technology

These determination methods have their own limitations, such as (1) require a pure culture state, and the rest of the methods are estimates of microbial activities or special components in a certain system, and the influence of other interfering substrates in the system on the results cannot be ruled out. Therefore, it cannot be accurately quantified and the error range of the estimation cannot be known.

Images