On-line cell microscopic observer for biological reactor

Abstract

The invention discloses an on-line cell microscopic observer for a biological reactor. The on-line cell microscopic observer is characterized by comprising an observer main body, a variable-focus optical system, a sampling device, a cleaning mechanism, an inner light source structure and a controller, wherein the observer main body is connected with the biological reactor through a fixing nut; three holes connected with the variable-focus optical system, the sampling device and the cleaning mechanism respectively are formed in the observer main body; the inner light source structure is connected with the observer main body through a telescopic rod; and the cleaning mechanism is connected with the observer main body through a movable shaft. By a mode of coupling a variable-focus objective lens and a front group of objective lenses, only a single camera can meet a dynamic observation requirement for simultaneously observing biological cells and animal cells. Furthermore, some basic technical requirements on the high-resolution optical system, high-speed image transmission capacity, high-efficiency cell number counting, high temperature resistance, high pressure resistance, automatic cleaning for the lenses and automatic collection of samples of the on-line cell microscopic observer are met.

Description

technical field [0001] The invention relates to an on-line cell microscope observer, in particular to an on-line cell morphology observer applied to a bioreactor. Background technique [0002] When microbial cells and animal cells are cultured in bioreactors, in order to understand their growth and morphological changes in real time, the most direct and effective method is to sample and analyze them. The traditional method uses off-line interval sampling, which is difficult to avoid the problem of bacterial contamination, and the observation dynamics are poor, and the process analysis is difficult. In response to these problems, researchers have developed a variety of online microscopic observers (In-situ microscope). [0003] German patent (Patent No. DE 4032002) introduces the design of a line cell observer, which is characterized by a transparent hole on the side wall of the bioreactor, and a light source is injected from the outside, and after being reflected by the obs...

AI Technical Summary

Problems solved by technology

Because the observer adopts the dark field illumination method of external light source, the optical system is complex and the cost is high

In addition, during the use of the observer, the cleaning mechanism and the sampling mechanism are easy to get stuck with each other after long-term use, requiring maintenance and calibration, and the reliability of the structure needs to be improved

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