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Microbial flocculating agent, and preparation and use thereof

A technology of microbial flocculants and strains, applied in the field of biological treatment of microorganisms and industrial wastewater, can solve the problems of difficult removal, small sedimentation particle size, etc., and achieve the effects of easy sedimentation and removal, large sedimentation particles, and high flocculation efficiency.

Active Publication Date: 2014-05-07
中国中化股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention is committed to developing a new microbial flocculant to solve the problems of the existing microbial flocculants after flocculation, such as small particle size and difficult removal.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The acquisition of two kinds of bacillus in embodiment 1

[0023] Bacillus megaterium SY-Z5 was isolated from soil samples from the seaside of Bohai Bay.

[0024] Primary screening medium components (g / L):

[0025] NaNO 3 3. FeSO 4 0.01, MgSO 4 ·7H 2 O0.5, K 2 HPO 4 1.0, KCl0.5, sucrose 30, agar powder 18, pH natural. Primary screening method: Put 5g of the collected soil sample in a 150mL Erlenmeyer flask filled with 50mL of distilled water, add a small amount of glucose and ammonium sulfate, shake and culture at 30°C for 3 days, and then serially dilute the culture solution to 10 -5 、10 -6 、10 -7 , use a glass rod to spread on the primary screening medium, culture at 30°C for 2-4 days, pick fast-growing and viscous strains for streak purification, and inoculate the pure strains on the slant medium (the components are the same as the primary screening medium ), stored at 4°C for later use.

[0026] Re-screening medium components (g / L):

[0027] Glucose 20, ...

Embodiment 2

[0036] The preparation of embodiment 2 microbial flocculants

[0037] Inoculate the SY-Z5 strain on the slant into 100mL seed medium (the composition is the same as the re-screening medium in Example 1), shake and cultivate overnight at 30°C and 150rpm, and insert the expanded fermentation medium with 5% inoculum ( The composition is the same as that of the re-screening medium), and continued to expand the culture for 3 days under the same conditions, and the concentration of bacteria detected by the dilution plate method reached 10 8 -10 10 CFU / mL.

[0038] Similarly, inoculate a loop of SY-ND strain into 100 mL of seed medium, and cultivate overnight at 37° C. with shaking at 150 rpm. Take the seed solution and insert it into the expanded fermentation medium with a 5% inoculum amount, and cultivate it for 3 days under the same conditions.

[0039] Seed medium components (g / L): peptone 10, glucose 5, yeast extract 3, NaCl5, pH 7.0.

[0040] Expanded fermentation medium co...

Embodiment 3

[0041] Example 3 Microbial flocculant application test

[0042] Prepare 2g / L white charcoal, bentonite and diatomaceous earth respectively, add microbial flocculant SY-Z5 strain fermentation broth 1%, SY-ND strain fermentation broth 1%, CaCl2 addition 0.01%, 1% NaOH to adjust the pH to 8.0, stirring treatment, fast stirring (250rpm) for 1min, slow stirring (80rpm) for 3min and then standing for 5min, most of the suspended matter is flocculated out, and the formed floc settles quickly. The test results show that the flocculation rates of the microbial flocculant of the present invention to the suspensions of white carbon, bentonite and diatomite are 87.7%, 91.7% and 95.4%, respectively.

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Abstract

The invention provides a microbial flocculating agent prepared through using two bacilli. The bacilli comprise Bacillus megaterium and Bacillus subtilis, wherein the Bacillus subtilis can generate a high-molecular biobased material gamma-polyglutamic acid, and two broths of the two bacilli are mixed and are added with a suspension substance to flocculate the suspension substance and generate a solid flocculating constituent. The microbial flocculating agent can substitute chemical flocculating agents, can be safely used in various industrial sewage processing industries, the food industry and the medicine industry, and has a popularization prospect.

Description

technical field [0001] The invention belongs to the field of biological treatment of microorganisms and industrial wastewater, and relates to a microbial flocculant and its preparation and application. technical background [0002] Water treatment agents are the focus of pharmaceutical research in the environmental protection industry, and flocculants, which play an important role in the solid-liquid separation of domestic sewage and industrial wastewater treatment, are even more prominent. It is known that inorganic polymers and organic synthetic polymer flocculants represented by polyacrylamide have secondary pollution characteristics, which pose a strong threat to the environment and human health; while biological flocculants are typical environmentally friendly water treatment agents, safe and non-toxic. It is toxic, biodegradable, has no secondary pollution to the ecology, and can increase the content of soil organic matter. It has good environmental benefits and will b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12N1/20C12R1/11C12R1/125
Inventor 胥维昌王芳朱希坤李小明张弘
Owner 中国中化股份有限公司
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