Collagen/chitosan micro-nano fiber composite hemostatic membrane material and preparation method thereof
A fiber composite and chitosan technology, applied in the fields of medical science, absorbent pads, bandages, etc., can solve the problems of insufficient mechanical properties of collagen-based hemostatic agents, unable to meet market requirements, insufficient purity of type I collagen, etc. Degradability, Wound Healing Promotion, Good Biocompatibility Effects
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[0028] Example 1
[0029] (1) Extraction of type I collagen: Take 10 parts by weight of medical biological skin sheet, cut it into small pieces of 0.5cm×0.5cm, wash 5 times with ultrapure water, and then soak in 0.05mol / L Tris, 1mol / L NaCl, pH 7.5 Tris-NaCl buffer solution, placed in an ultrasonic cleaner with a frequency of 20kHz for 2h; pour off the buffer and rinse the skin twice with distilled water; add 30 times 0.5M acetic acid Soak the solution for 2 hours, break it with a homogenizer at a constant temperature of 4℃, homogenize, then transfer to the reactor, add 0.2 weight part of pepsin; under the condition of ultrasonic frequency of 30kHz, slowly stirring at 4℃ Enzymatic hydrolysis for 36 hours, 1 hour every 3 hours; after the reaction is completed, stop stirring, filter the reaction solution, adjust the pH of the filtrate to 7.0, add ammonium sulfate powder with a final concentration of 1.5 mol / L, and let stand for 10 hours; set the collagen solution at 10000 rpm Cen...
Example Embodiment
[0035] Example 2
[0036] (1) Extraction of type I collagen: Take 10 parts by weight of medical biological skin sheet, cut it into small pieces of 0.5cm×0.5cm, wash 5 times with ultrapure water, and then soak in 0.05mol / L Tris, 1mol / L NaCl, pH 7.5 Tris-NaCl buffer solution, placed in an ultrasonic cleaner with a frequency of 30kHz, for 2h; pour off the buffer, rinse the skin twice with distilled water; add 40 times 0.5M acetic acid Soak the solution for 2 hours, crush and homogenize with a homogenizer at a constant temperature of 4°C, then transfer to the reactor, add 0.2 parts by weight of pepsin, and slowly stir the enzyme at 4°C under ultrasonic conditions with a frequency of 30kHz Solution 24h, 1h every 3h. After the reaction is complete, stop stirring, filter the reaction solution with suction, adjust the pH of the filtrate to 7.5, add ammonium sulfate powder with a final concentration of 1.5 mol / L, and let stand for 15 hours; centrifuge the collagen solution at 20,000 rp...
Example Embodiment
[0042] Example 3
[0043] (1) Extraction of type I collagen: Take 10 parts by weight of medical biological skin sheet, cut it into small pieces of 0.5cm×0.5cm, wash 5 times with ultrapure water, and then soak in 0.05mol / L Tris, 1mol / L NaCl, pH 7.5 Tris-NaCl buffer solution, placed in an ultrasonic cleaner with a frequency of 30kHz, for 2h; pour off the buffer, rinse the skin twice with distilled water; add 50 times 0.5M acetic acid Soak the solution for 2 hours, break it with a homogenizer at a constant temperature of 4°C, homogenize it, then transfer it to the reactor, add 0.2 parts by weight of pepsin; stir the enzyme slowly at 4°C under ultrasonic conditions with a frequency of 20kHz Resolve for 36h, and act for 1h every 3h; after the reaction is complete, stop stirring, filter the reaction solution with suction, adjust the pH of the filtrate to 7.3, add ammonium sulfate powder with a final concentration of 1.5mol / L, and let stand for 20h. Centrifuge the collagen solution ...
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