Collagen/chitosan micro-nano fiber composite hemostatic membrane material and preparation method thereof

A fiber composite and chitosan technology, applied in the fields of medical science, absorbent pads, bandages, etc., can solve the problems of insufficient mechanical properties of collagen-based hemostatic agents, unable to meet market requirements, insufficient purity of type I collagen, etc. Degradability, Wound Healing Promotion, Good Biocompatibility Effects

Active Publication Date: 2013-08-07
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. The existing collagen-based hemostatic agent has insufficient mechanical properties and poor adhesion, which causes unnecessary troubles to the operation;
[0007] 2. The existing collagen-based hemostatic agents have general hemostatic performance and single function, which can no

Method used

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  • Collagen/chitosan micro-nano fiber composite hemostatic membrane material and preparation method thereof
  • Collagen/chitosan micro-nano fiber composite hemostatic membrane material and preparation method thereof
  • Collagen/chitosan micro-nano fiber composite hemostatic membrane material and preparation method thereof

Examples

Experimental program
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Example Embodiment

[0028] Example 1

[0029] (1) Extraction of type I collagen: Take 10 parts by weight of medical biological skin sheet, cut it into small pieces of 0.5cm×0.5cm, wash 5 times with ultrapure water, and then soak in 0.05mol / L Tris, 1mol / L NaCl, pH 7.5 Tris-NaCl buffer solution, placed in an ultrasonic cleaner with a frequency of 20kHz for 2h; pour off the buffer and rinse the skin twice with distilled water; add 30 times 0.5M acetic acid Soak the solution for 2 hours, break it with a homogenizer at a constant temperature of 4℃, homogenize, then transfer to the reactor, add 0.2 weight part of pepsin; under the condition of ultrasonic frequency of 30kHz, slowly stirring at 4℃ Enzymatic hydrolysis for 36 hours, 1 hour every 3 hours; after the reaction is completed, stop stirring, filter the reaction solution, adjust the pH of the filtrate to 7.0, add ammonium sulfate powder with a final concentration of 1.5 mol / L, and let stand for 10 hours; set the collagen solution at 10000 rpm Cen...

Example Embodiment

[0035] Example 2

[0036] (1) Extraction of type I collagen: Take 10 parts by weight of medical biological skin sheet, cut it into small pieces of 0.5cm×0.5cm, wash 5 times with ultrapure water, and then soak in 0.05mol / L Tris, 1mol / L NaCl, pH 7.5 Tris-NaCl buffer solution, placed in an ultrasonic cleaner with a frequency of 30kHz, for 2h; pour off the buffer, rinse the skin twice with distilled water; add 40 times 0.5M acetic acid Soak the solution for 2 hours, crush and homogenize with a homogenizer at a constant temperature of 4°C, then transfer to the reactor, add 0.2 parts by weight of pepsin, and slowly stir the enzyme at 4°C under ultrasonic conditions with a frequency of 30kHz Solution 24h, 1h every 3h. After the reaction is complete, stop stirring, filter the reaction solution with suction, adjust the pH of the filtrate to 7.5, add ammonium sulfate powder with a final concentration of 1.5 mol / L, and let stand for 15 hours; centrifuge the collagen solution at 20,000 rp...

Example Embodiment

[0042] Example 3

[0043] (1) Extraction of type I collagen: Take 10 parts by weight of medical biological skin sheet, cut it into small pieces of 0.5cm×0.5cm, wash 5 times with ultrapure water, and then soak in 0.05mol / L Tris, 1mol / L NaCl, pH 7.5 Tris-NaCl buffer solution, placed in an ultrasonic cleaner with a frequency of 30kHz, for 2h; pour off the buffer, rinse the skin twice with distilled water; add 50 times 0.5M acetic acid Soak the solution for 2 hours, break it with a homogenizer at a constant temperature of 4°C, homogenize it, then transfer it to the reactor, add 0.2 parts by weight of pepsin; stir the enzyme slowly at 4°C under ultrasonic conditions with a frequency of 20kHz Resolve for 36h, and act for 1h every 3h; after the reaction is complete, stop stirring, filter the reaction solution with suction, adjust the pH of the filtrate to 7.3, add ammonium sulfate powder with a final concentration of 1.5mol / L, and let stand for 20h. Centrifuge the collagen solution ...

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Abstract

The invention discloses a collagen/chitosan micro-nano fiber composite hemostatic membrane material and a preparation method thereof. The preparation method is characterized by comprising the following steps of: extracting a medical biological skin sheet serving as a raw material by an ultrasonic technology to prepare I type collagen; mixing hexafluoroisopropanol and acetic acid according to different volume ratios to prepare a spinning solvent; mixing the prepared I type collagen and chitosan in a certain mass ratio and adding the mixture into the spinning solvent; stirring in an ultrasonic cleaner at room temperature until the material is transparent to prepare electrostatic spinning mother liquid at the concentration of 4 to 10 percent; injecting the electrostatic spinning mother liquid into an electrostatic spinning machine and performing electrostatic spinning to obtain a collagen/chitosan micro-nano fiber composite membrane; soaking the collagen/chitosan micro-nano fiber composite membrane into natural biomacromolecules and Chinese herbal medicines sequentially; and freeze-drying to obtain the collagen/chitosan micro-nano fiber composite hemostatic membrane material. The collagen/chitosan micro-nano fiber composite hemostatic membrane material has excellent biocompatibility, biodegradability, adhesion property and the like, can quickly stop bleeding, resist inflammation, ease pain and promote wound healing, and can be applied to trauma hemostasis and repair and general civil hemostasis emergency treatment.

Description

technical field [0001] The invention relates to a hemostatic agent which has excellent properties such as good biocompatibility, biodegradability and adhesion, and can quickly stop bleeding, anti-inflammatory and analgesic, and promote wound compounding. Background technique [0002] Bleeding poses a great danger to human life, and the search for fast and effective hemostatic agents has always been a research hotspot at home and abroad. In various surgical operations or trauma treatments, wound bleeding and isolation from the outside world to avoid infection have a great impact on the wound healing of patients. In war, according to statistics, bleeding is the main cause of death within 48 hours after injury, accounting for 80% of all traumatic accidents. Excessive traumatic blood loss will cause pain, shock, coma and even death of the wounded. Therefore, hemostatic materials need to stop bleeding quickly and effectively, and must also have anti-inflammatory and analgesic ef...

Claims

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Application Information

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IPC IPC(8): A61L15/32A61L15/28A61L15/44
Inventor 但年华刘新华但卫华胡杨刘婷
Owner SICHUAN UNIV
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