Bacillus lentus and method for generating by inducing bacillus lentus for reinforcing foundation
A technology of Bacillus lentus and calcium carbonate, applied in the field of foundation reinforcement, can solve the problems of small radius, large impact, low economic performance, etc., and achieve the effects of low cost, improved strength time, and good effect.
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[0032] The present invention will be further described in detail below with reference to the drawings and specific embodiments.
[0033] Isolation and Screening of Strains Producing Urease Induced Calcium Carbonate
[0034] The urease-producing strain was isolated from the flower garden of Tsinghua University to collect soil samples.
[0035] Bacteria have urea decomposing enzyme, which can decompose urea to produce a large amount of ammonia, making the culture medium alkaline and red. This experiment takes advantage of this feature. The test sample is first enriched and cultured at 37°C and 5mol / L of high-concentration urea for 24 hours to kill various microbial vegetative cells that cannot tolerate and use high-concentration urea. Dilute the treated culture solution stepwise, spread it on a urease screening culture plate, and cultivate it at 37°C. Pick the strains that make the culture medium red, streak and separate a single colony. The obtained microorganism is a urease-producin...
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