Bacillus lentus and method for generating by inducing bacillus lentus for reinforcing foundation

A technology of Bacillus lentus and calcium carbonate, applied in the field of foundation reinforcement, can solve the problems of small radius, large impact, low economic performance, etc., and achieve the effects of low cost, improved strength time, and good effect.

Inactive Publication Date: 2013-08-28
TSINGHUA UNIV
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  • Application Information

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Problems solved by technology

However, the traditional geotextile reinforcement technology has the problem of insufficient durability; the chemical grouting based on cement or lime or organic glue has a great impact on the environment, and greatly reduces the water permeability of the foundation, hindering the seepage of ground

Method used

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  • Bacillus lentus and method for generating by inducing bacillus lentus for reinforcing foundation
  • Bacillus lentus and method for generating by inducing bacillus lentus for reinforcing foundation
  • Bacillus lentus and method for generating by inducing bacillus lentus for reinforcing foundation

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Embodiment Construction

[0032] The present invention will be further described in detail below with reference to the drawings and specific embodiments.

[0033] Isolation and Screening of Strains Producing Urease Induced Calcium Carbonate

[0034] The urease-producing strain was isolated from the flower garden of Tsinghua University to collect soil samples.

[0035] Bacteria have urea decomposing enzyme, which can decompose urea to produce a large amount of ammonia, making the culture medium alkaline and red. This experiment takes advantage of this feature. The test sample is first enriched and cultured at 37°C and 5mol / L of high-concentration urea for 24 hours to kill various microbial vegetative cells that cannot tolerate and use high-concentration urea. Dilute the treated culture solution stepwise, spread it on a urease screening culture plate, and cultivate it at 37°C. Pick the strains that make the culture medium red, streak and separate a single colony. The obtained microorganism is a urease-producin...

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Abstract

The invention provides bacillus lentus and a method for generating by inducing bacillus lentus for reinforcing foundation, the bacillus lentus URIC, the bacillus lentus UR9D, and the bacillus lentus UR52 are preserved in China General Microbiological Culture Collection Center (CGMCC) on August 29, 2012; the method comprises: firstly, respectively fermenting and culturing a single bacterium colony of bacillus lentus in a fermentation medium, then grouting in two steps, filling bacteria liquid into a sand column pumped with deionized water, and filling a mixed solution of reaction solution calcium chloride and urea, to form a microbe-calcium carbonate-sand grains consolidating body in a sand grain-solution system, and furthermore to enhance bonding degree and intensity of the sand grains. The bacillus lentus and the method of the invention has advantages of short solidification time, good effect and low cost, and will not cause pollution on environment.

Description

Technical field [0001] The invention relates to the technical field of foundation reinforcement, in particular to Bacillus lentus and a method for inducing calcium carbonate to be produced for foundation reinforcement. Background technique [0002] Foundation reinforcement is an extensive and important engineering practice activity in civil and geotechnical engineering. Insufficient bearing capacity of the foundation soil will lead to the settlement of roads and railways, the instability of dams, dunes and slopes, and excessive erosion of coasts or river banks. Earthquakes or traffic vibrations can cause liquefaction of soft and loose foundation soil. Oil and gas drilling projects or excavation of foundation pits in loose rock and soil are often severely affected by quicksand and fluid soil. In economically developed deltas, land reclamation, reinforcement of weak foundations, and improvement of foundation density and stability are also important issues. [0003] Traditional che...

Claims

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Application Information

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IPC IPC(8): C12N1/20E02D3/12C12R1/07
Inventor 程晓辉化彬李萌
Owner TSINGHUA UNIV
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