Universal rapid detection method for micromolecule poisonous and harmful materials in powdery food

Abstract

The invention discloses a universal rapid detection method for micromolecule poisonous and harmful materials in powdery food. The method is characterized by comprising the following steps of performing simple and universal extraction and purification on a liquid milk sample to obtain a loading solution; preparing a mixed standard solution and making a matrix-adding standard curve; then measuring the micromolecule poisonous and harmful materials in the liquid milk by high performance liquid chromatography-triple quadrupole tandem mass spectrometry combination; and finally performing concentration calculation. The method has the advantages that the pretreatment and instrumental analysis processes have strong compatibility for compounds with different physicochemical properties; detection efficiency is high; operability is strong; detection limit can meet all subjected target objects; and detection cost is reduced.

Description

technical field [0001] The invention relates to a detection method of small molecule toxic and harmful substances in powdered food, in particular to an ultra-trace amount of small molecule toxic and harmful substances (such as veterinary drugs, environmental hormones, etc.) , biological toxins, pesticides, additives, illegal additives, etc., molecular weight less than 1500) liquid chromatography-tandem mass spectrometry or liquid chromatography-high resolution mass spectrometry and other related detection techniques for general rapid detection methods. Background technique [0002] Dry powdered foods such as infant formula milk powder, milk powder, rice flour, and oatmeal are nutritional products with huge consumption. There are many factors affecting its quality and safety. Among them, a wide variety of small molecular organic hazards (molecular weight less than 2000) with different properties pose different degrees of hidden dangers to consumers’ health, including veterina...

AI Technical Summary

Problems solved by technology

[0004] At present, although there are a large number of current national standards and industry standard detection methods for these toxic and harmful substances in powdered foods in my country, these methods are divided into multiple sample preparation methods, with too many operating steps, and the detection personnel are distracted, which is not only time-consuming It is laborious, the detection efficiency is low, the operability is not strong, and the coverage of the project is not comprehensive, it is easy to bring the risk of missed detection, the cycle is long, the experimental cost is high, and it is difficult to complete the efficient detection and detection of toxic and harmful substances in powdered food. Supervision

The reason is that the pretreatment of these methods is incompatible with compounds of different physical and chemical properties and lacks versatility, so it is difficult to significantly improve the monitoring efficiency in conventional methods

Images